Effects of putative diuretic factors on intracellular second messenger levels in the Malpighian tubules of Aedes aegypti

Cady, Craig; Hagedorn, Henry H.

doi:10.1016/s0022-1910(98)00130-9

Cited by 38 publications

(32 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, inconsistent with a role for PKA is the lack of effect of any aedeskinin on intracellular cAMP levels after 2 min of stimulation (Fig. 5), which confirms previous observations of cAMPindependent diuretic effects of (1) tubules (Cady and Hagedorn, 1999) and (2) drosokinin in Drosophila Malpighian tubules (Terhzaz et al, 1999).…”

Section: Discussionsupporting

confidence: 88%

Signaling to the apical membrane and to the paracellular pathway: changes in the cytosolic proteome ofAedesMalpighian tubules

Beyenbach

Baumgart

Lau

et al. 2009

Journal of Experimental Biology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 88%

Signaling to the apical membrane and to the paracellular pathway: changes in the cytosolic proteome ofAedesMalpighian tubules

Beyenbach

Baumgart

Lau

et al. 2009

Journal of Experimental Biology

View full text Add to dashboard Cite

“…Aedes leucokinins have been shown to stimulate IP 3 production in mosquito tubules (Cady and Hagedorn, 1999). However, as Drosokinin exerts its effects solely via stellate cells in Drosophila tubules (Terhzaz et al, 1999), and as Drosophila tubules each contain only 22 stellate cells , Drosokinin-stimulated IP3 levels could not be reliably quantified in intact tubules (data not shown).…”

Section: Plcβ Contributes To Cap2b and Drosokinin-induced Fluidmentioning

confidence: 91%

norpAanditprmutants reveal roles for phospholipase C and inositol (1,4,5)- trisphosphate receptor inDrosophila melanogasterrenal function

Pollock

Radford

Pyne

et al. 2003

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYMutants of norpA, encoding phospholipase Cβ (PLCβ), and itpr, encoding inositol (1,4,5)-trisphosphate receptor(IP3R), both attenuate response to diuretic peptides of Drosophila melanogaster renal (Malpighian) tubules. Intact tubules from norpA mutants severely reduced diuresis stimulated by the principal cell- and stellate cell-specific neuropeptides, CAP2b and Drosophila leucokinin (Drosokinin), respectively, suggesting a role for PLCβ in both these cell types. Measurement of IP3production in wild-type tubules and in Drosokinin-receptor-transfected S2 cells stimulated with CAP2b and Drosokinin, respectively, confirmed that both neuropeptides elevate IP3 levels.In itpr hypomorphs, basal IP3 levels are lower,although CAP2b-stimulated IP3 levels are not significantly reduced compared with wild type. However,CAP2b-stimulated fluid transport is significantly reduced in itpr alleles. Rescue of the itpr90B.0 allele with wild-type itpr restores CAP2b-stimulated fluid transport levels to wild type. Drosokinin-stimulated fluid transport is also reduced in homozygous and heteroallelic itpr mutants.Measurements of cytosolic calcium levels in intact tubules of wild-type and itpr mutants using targeted expression of the calcium reporter,aequorin, show that mutations in itpr attenuated both CAP2b- and Drosokinin-stimulated calcium responses. The reductions in calcium signals are associated with corresponding reductions in fluid transport rates.Thus, we describe a role for norpA and itpr in renal epithelia and show that both CAP2b and Drosokinin are PLCβ-dependent, IP3-mobilising neuropeptides in Drosophila. IP3R contributes to the calcium signalling cascades initiated by these peptides in both principal and stellate cells.

show abstract

“…The diuretic hormones belong to the family of sauvagine/corticotropin-releasing factor/urotensin I-related insect diuretic peptides (Furuya et al, 1995(Furuya et al, , 1998. The two hormones increase the production of cAMP, the nucleotide that is widely believed to mediate diuresis in insects (Beyenbach, 2003;Cady and Hagedorn, 1999;Hazelton et al, 2002;O'Donnell et al, 1996;Xu and Marshall, 2000). Cyclic AMP increases fluid secretion in Malpighian tubules of Aedes aegypti by increasing a Na + -conductance and bumetanide-sensitive transport system with the effect of stimulating transepithelial NaCl secretion, and not KCl secretion (Hegarty et al, 1991;Sawyer and Beyenbach, 1985).…”

Section: Diuretic and Antidiuretic Factors Of Tenebriomentioning

confidence: 99%

The mechanism of action of the antidiuretic peptide Tenmo ADFa in Malpighian tubules ofAedes aegypti

Massaro

Lee

Patel

et al. 2004

Journal of Experimental Biology

View full text Add to dashboard Cite

Effects of putative diuretic factors on intracellular second messenger levels in the Malpighian tubules of Aedes aegypti

Cited by 38 publications

References 51 publications

Signaling to the apical membrane and to the paracellular pathway: changes in the cytosolic proteome ofAedesMalpighian tubules

Signaling to the apical membrane and to the paracellular pathway: changes in the cytosolic proteome ofAedesMalpighian tubules

norpAanditprmutants reveal roles for phospholipase C and inositol (1,4,5)- trisphosphate receptor inDrosophila melanogasterrenal function

The mechanism of action of the antidiuretic peptide Tenmo ADFa in Malpighian tubules ofAedes aegypti

Contact Info

Product

Resources

About