Effects of putrescine accumulation in tobacco transgenic plants with different expression levels of oat arginine decarboxylase

Panicot, Mireia; Masgrau, Carles; Borrell, Antonio; Cordeiro, A. R.; Tiburcio, Antonio F.; Altabella, Teresa

doi:10.1034/j.1399-3054.2002.1140214.x

Cited by 34 publications

(26 citation statements)

References 32 publications

(75 reference statements)

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“…In the present experiment, Ni increased the Put level and markedly decreased Spd and Spm levels, and these changes were accompanied by the substantial generation of ROS. Although some experiments have shown that Put has the ability to scavenge ROS in vitro (Sharma and Dietz 2006), it should be noted that a mass accumulation of Put is generally considered toxic to plants and eventually leads to apoptotic cell death if its level becomes too high (Panicot et al 2002;Takao et al 2006). It has often been suggested that Put and derived PAs (Spd, Spm) may have distinct functions in response to stress.…”

Section: Discussionmentioning

confidence: 98%

Polyamines content and physiological and biochemical responses to ladder concentration of nickel stress in Hydrocharis dubia (Bl.) Backer leaves

2008

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Influence of ladder concentration of nickel (Ni) on the leaves of Hydrocharis dubia were studied after 3 days treatment. The accumulation of Ni, the content of polyamines, proline, malondialdehyde (MDA) and soluble protein, as well as the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the leaves were investigated. The result indicated that the toxicity of Ni manifested in respective aspect of physiological and biochemical characters. Significant increase of Ni concentration in the leaf tissue was observed, which was concentration dependent. Visible symptoms of Ni toxicity: chlorosis and necrosis occurred following the 3rd day. Meantime, treatment with Ni resulted in the increase in the generation rate of O2(*-) in the leaves. SOD and CAT activities decreased significantly in response to Ni treatment, it was possibly the reason of accumulation of O2(*-). However, a several-fold decrease in POD activities was found. Our results indicated that because of prolonged increases in O2(*-) level, oxidative damage, measured as the level of lipid peroxidation, occured in the leaves of Ni treated fronds. The changes of the content of polyamines (PAs) were also investigated in the leaves of Hydrocharis dubia. Ni treatment significantly increased the putrescine (Put) level and lowered spermidine (Spd) and spermine (Spm) levels, thereby significantly reducing the ratio of free (Spd + Spm)/Put in leaves, which has been considered as the signal under stress. Although the trend that PS-conjugated PAs and PIS-bound PAs changed the same as free PAs, they changed in more less extent.

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Section: Discussionmentioning

confidence: 98%

Polyamines content and physiological and biochemical responses to ladder concentration of nickel stress in Hydrocharis dubia (Bl.) Backer leaves

2008

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“…This raises the possibility that embryonic arrest in the double mutant is associated with toxicity of overaccumulated putrescine. Previous studies have shown that overexpression of oat ADC in tobacco leads to abnormal phenotypes such as short internodes, thin stems and leaves, leaf necrosis, and reduced root growth, the severity of which is correlated with putrescine content (Masgrau et al, 1997;Panicot et al, 2002a). In contrast, Noury et al (2000) generated transgenic rice plants that show normal growth but up to a 10-fold increase in the amount of putrescine in the seeds compared to that in wild-type seeds by overexpressing the oat ADC cDNA.…”

Section: Discussionmentioning

confidence: 99%

Spermidine Synthase Genes Are Essential for Survival of Arabidopsis

et al. 2004

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The cellular polyamines putrescine, spermidine, and spermine are ubiquitous in nature and have been implicated in a wide range of growth and developmental processes. There is little information, however, on mutant plants or animals defective in the synthesis of polyamines. The Arabidopsis genome has two genes encoding spermidine synthase, SPDS1 and SPDS2. In this paper, we describe T-DNA insertion mutants of both of these genes. While each mutant allele shows normal growth, spds1-1 spds2-1 double-mutant seeds are abnormally shrunken and they have embryos that are arrested morphologically at the hearttorpedo transition stage. These seeds contain significantly reduced levels of spermidine and high levels of its precursor, putrescine. The embryo lethal phenotype of spds1-1 spds2-1 is complemented by the wild-type SPDS1 gene. In addition, we observed a nearly identical seed phenotype among an F 2 seed population from the cross between the spds2-1 allele and SPDS1 RNA interference transgenic lines. These data provide the first genetic evidence indicating a critical role of the spermidine synthase in plant embryo development.

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“…Similar results were obtained in adc tobacco and rice transgenic plants (Burtin and Michael 1997;Capell et al 2004) and in rice (Roy and Wu 2001), tobacco (Franceschetti et al 2004), Arabidopsis (Kasukabe et al 2004) and sweet potato (Kasukabe et al 2006) transgenic plants over-expressing other PA biosynthetic genes, samdc and Spd synthase. However, morphological abnormalities such as short internodes and wrinkled leaves have been reported in transgenic tobacco plants over-expressing odc (DeScenzo and Minocha 1993; Kumria and Rajam 2002), adc (Masgrau et al 1997;Panicot et al 2002) and samdc (Noh and Minocha 1994;Waie and Rajam 2003) genes. Our results and the published work indicate that the phenotypic abnormalities in transgenic plants over-expressing PA genes might depend upon the transgene expression and cellular of PA levels.…”

Section: Transformation and Regenerationmentioning

confidence: 99%

Polyamine accumulation in transgenic eggplant enhances tolerance to multiple abiotic stresses and fungal resistance

Prabhavathi

Rajam

2007

Plant Biotechnology

112

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for stress responses (reviewed by Rajam et al. 1998;Kumar et al. 2006). It has been reported that the overexpression of PA biosynthetic genes like adc (Roy and Wu 2001;Capell et al. 1998;2004) Abstract Polyamines (putrescine, spermidine and spermine) have been shown to be important in stress tolerance. Thus, the present study was undertaken with the aim to enhance stress tolerance in eggplant by introduction of a key polyamine biosynthetic gene arginine decarboxylase, adc under the control of a constitutive promoter of cauliflower mosaic virus, CaMV35S through Agrobacterium-mediated transformation. Several putative transgenic plants were generated and the transgene integration and expression was confirmed by PCR and Southern blot analyses, and RT-PCR analysis, respectively. These transgenic plants have shown an enhanced level of polyamines due to the increase in ADC enzyme activity. The diamine oxidase (an enzyme involved in putrescine and spermidine degradation) activity was also increased in these transgenic plants. Polyamine-accumulating transgenic plants exhibited an increased tolerance levels to multiple abiotic stresses such as salinity, drought, low and high temperature, and heavy-metal and resistance against fungal wilt disease caused by Fusarium oxysporium.Key words: Agrobacterium, arginine decarboxylase, eggplant, plant transformation, polyamines, stress tolerance.Plant Biotechnology 24, 273-282 (2007) Original PaperAbbreviations: ADC, arginine decarboxylase; DAO, diamine oxidase; MS, Murashige and Skoog; ODC, ornithine decarboxylase; PAs, polyamines; Put, putrescine; Spd, spermidine; Spm, spermine. This article can be found at http://www,jspcmb.jp/ Kasukabe et al. 2004; in plants like rice, tobacco, Arabidopsis and sweet potato, has resulted in increased tolerance to abiotic stresses. There is only one report on fungal resistance in transgenic tobacco plants overexpressing samdc gene (Waie and Rajam 2003).Therefore, the present work was aimed to generate transgenic eggplants with oat arginine decarboxylase (ADC, EC 4.1.1.19) gene, a key gene involved in PA biosynthesis, and evaluate such transgenic plants for PA levels and tolerance against both abiotic and biotic stresses under in vitro and in vivo growth conditions. Indeed, these transgenic eggplants have shown elevated PA content that could be correlated with increased tolerance to multiple abiotic stresses and fungal resistance. Materials and methods Plant material and plasmidThe seeds of eggplant (Solanum melongena L., cv. Pusa Purple Long) were obtained from the National Seeds Corporation, Indian Agricultural Research Institute, New Delhi. The Agrobacterium tumefaciens strain LBA4404 containing binary plasmid pMVR3ADC with oat adc gene under the control of cauliflower mosaic virus CaMV35S promoter and nopaline synthase terminator, and hygromycin phosphotransferase (hpt) as plant selection marker was used for eggplant transformation ( Figure 1A). Eggplant transformation and regenerationLeaf and cotyledon explants, collected from about thirty ...

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Effects of putrescine accumulation in tobacco transgenic plants with different expression levels of oat arginine decarboxylase

Cited by 34 publications

References 32 publications

Polyamines content and physiological and biochemical responses to ladder concentration of nickel stress in Hydrocharis dubia (Bl.) Backer leaves

Polyamines content and physiological and biochemical responses to ladder concentration of nickel stress in Hydrocharis dubia (Bl.) Backer leaves

Spermidine Synthase Genes Are Essential for Survival of Arabidopsis

Polyamine accumulation in transgenic eggplant enhances tolerance to multiple abiotic stresses and fungal resistance

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