Effects of putrescine and inhibitors of putrescine biosynthesis on organogenesis inEuphorbia esula L.

Davis, David W.; Olson, Prudence A.

doi:10.1007/bf02632141

Cited by 8 publications

(10 citation statements)

References 26 publications

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“…Estas constatações encontram respaldo na literatura, uma vez que várias rotas morfogenéticas (SAIPRASAD et al, 2004;DAVIS & OLSON, 1994) e controles oxidativos (TANG et al, 2004) foram associados ao efeito direto de alguma poliamina suplementada ao meio de cultura durante o cultivo in vitro de diferentes espécies.…”

Section: Resultsunclassified

“…Esse fato não foi observado quando se aplicou as três poliaminas (T8) no meio de cultura e os valores encontrados foram inferiores aos observados quando se aplicou somente PUT (T4) exógena. DAVIS & OLSON (1994) O uso exógeno de SPD isolada (T2) ou combinada com SPM (T5) ou PUT (T6), nos calos mantidos em meio líquido por 30 dias (165 dias), forneceu material com alta porcentagem de oxidação. Observou-se relação negativa dos calos crescidos em meio contendo SPD, os quais apresentaram alta porcentagem de tecido oxidado (escuro/necrosado), com número e altura dos brotos formados, além de ausência de raízes, enquanto que a presença da PUT atenuou esse efeito.…”

Section: Resultsunclassified

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Estudo das poliaminas na morfogênese in vitro de Hemerocallis sp.

2007

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Em cultura de tecidos, ações de indução do desenvolvimento vegetal e também controle oxidativo de células e tecidos, podem ser atribuídas às poliaminas. Neste sentido, propôs-se investigar a participação das poliaminas espermidina (SPD), espermina (SPM) e putrescina (PUT) na indução da diferenciação de calos e no controle oxidativo de células e tecidos de Hemerocallis sp cultivados in vitro. Massas celulares, oriundas de calos produzidos in vitro, foram cultivadas em meio de cultura MS, suplementado com SPD, SPM e PUT em diferentes concentrações e combinações, avaliando-se teores endógenos de poliaminas e proteínas solúveis, atividade das peroxidases (EC 1.11.1.7), número e altura dos brotos, porcentagem de microbrotações, hiperhidricidade e necrose, e formação de calos. Os resultados apontam uma participação das poliaminas não só na indução da morfogênese, mas também no que se refere aos padrões oxidativos de células e tecidos. Ao final do experimento, a aplicação de PUT, isolada (10µM PUT) ou combinada com SPD (10µM SPD + 10 µM PUT) ou SPM (10µM SPM +10µM PUT), induziu as maiores médias em relação ao número e à altura de brotos diferenciados. Já a combinação das três poliaminas no meio de cultura (10µM SPD + 10µM SPM + 10µM PUT) induziu maior porcentagem (60%) de formação de microplantas nos calos. A combinação de SPD e SPM (10µM SPD + 10µM SPM) induziu maior quantidade de tecido necrosado (65%). Verificou-se que SPM aplicada exogenamente no meio de cultura (10µM SPM) pode atuar como antioxidante celular na cultura de calos desta espécie.

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Section: Resultsunclassified

Estudo das poliaminas na morfogênese in vitro de Hemerocallis sp.

2007

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“…The formation of roots and shoots in isolated hypocotyl segments of leafy spurge is inhibited by two inhibitors of putrescine biosynthesis, difluorylmethylornithine (DFMO) and difluoromethylarginine (DFMA) (Davis and Olson, 1994) that inhibit ODC and ADC, respectively (Slocum and Flores, 1991), and are reported to be specific suicide inhibitors of the two enzymes (McCann et al, 1987). A study similar to that of Davis and Olson (1994) was undertaken with canaline and canavanine, which are non-specific inhibitors of ADC and ODC, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…A study similar to that of Davis and Olson (1994) was undertaken with canaline and canavanine, which are non-specific inhibitors of ADC and ODC, respectively. If similar results would be obtained, strong argument might be made for the involvement of putrescine and the polyamines in organ formation.…”

Section: Introductionmentioning

confidence: 99%

Polyamines, auxins and organogenesis in leafy spurge (Euphorbia esula L.)

Davis¹

1997

Journal of Plant Physiology

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Summary:Canaline and canavanine (inhibitors of polyamine biosynthesis) effects on root and shoot formation were determined in vitro for etiolated hypocotyl segments of leafy spurge (Euphorbia esula L.). Canaline (an ornithine analogue) at 10 to 100 µmol/L inhibited root and shoot formation. The simultaneous application of putrescine did not reverse canaline-induced inhibition of root formation. Ornithine, a precursor of putrescine via the ornithine decarboxylase pathway, unexpectedly inhibited root formation. Canavanine (an arginine analogue) at 30 to 100 µmol/L inhibited root and/or shoot formation. Inhibition of root formation by canavanine was not reversed by agmatine or putrescine, but was partially reversed by arginine. Indoleacetic acid (IAA) inhibited shoot formation, stimulated root formation, and partially reversed the inhibition of root formation by either canaline or canavanine. IAA generally increased endogenous levels of free polyamines in controls and canaline-or canavanine-treated tissues, but these changes were small and probably had little effect on organogenesis. IAA also partially reversed the inhibition of root (but not shoot) formation by arcaine and pentamidine, inhibitors of polyamine action in mammals. The roles of the polyamines in plants is not clear, but the polyamines may play only minor roles in root and shoot formation.

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“…Indole-3-acetic acid (IAA) partially counteracts the inhibition of organogenesis in leafy spurge tissues resulting from treatment with 0.5 mM DFMO + 0.5 mM DFMA in the culture medium (Davis and Olson 1994). This treatment resulted in the depletion of endogenous putrescine within approximately 3 days.…”

Section: Introductionmentioning

confidence: 99%

2,4-Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

Davis¹

1997

Physiol Plant

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Abstract:Difluoromethylornithine (DFMO) is a well known inhibitor of putrescine biosynthesis that has been reported to interact in varying ways with auxins such as indoleacetic acid (IAA) and 2,4-dichlorophenoxy acetic acid (2,4-D). In the present report DFMO is shown to inhibit root formation in isolated hypocotyl segments of Euphorbia esula L. (leafy spurge) grown in the dark on solidified nutrient media in Petri dishes. Shoot formation was only slightly inhibited by DFMO and only on media with salts and vitamins diluted 10-fold. 2,4-D inhibited both root and shoot formation in full strength or diluted media. Simultaneous application of both compounds resulted in partial reversal of root inhibition, but only at 450 nM 2,4-D, the highest concentration used. In both media IAA also partially reversed DFMO effects on root formation. The effects of DFMO, 2,4-D or IAA on root (or shoot) formation do not appear to be closely related to endogenous content of the polyamines determined by high performance liquid chromatography. Abbreviations:DFMA, DL-α-difluoromethylarginine; DFMO, DL-α-difluoromethy-

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Effects of putrescine and inhibitors of putrescine biosynthesis on organogenesis inEuphorbia esula L.

Cited by 8 publications

References 26 publications

Estudo das poliaminas na morfogênese in vitro de Hemerocallis sp.

Estudo das poliaminas na morfogênese in vitro de Hemerocallis sp.

Polyamines, auxins and organogenesis in leafy spurge (Euphorbia esula L.)

2,4-Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

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