Effects of Pyrene on Human Liver HepG2 Cells: Cytotoxicity, Oxidative Stress, and Transcriptomic Changes in Xenobiotic Metabolizing Enzymes and Inflammatory Markers with Protection Trial Using Lycopene

Ma, Jin; Eldin, Walaa Fathy Saad; El‐Ghareeb, Waleed Rizk; Elhelaly, Abdelazim Elsayed; Khedr, Mariam H.E.; Li, Xiang; Huang, Xiaochen

doi:10.1155/2019/7604851

Cited by 20 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pyrene is known to generate ROS, which induces cell death. , Therefore, we measured the amount of ROS to elucidate the mechanism of cell death due to the presence of the pyrene group. The 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) assay is a precise technique for directly measuring the ROS in live cells.…”

Section: Resultsmentioning

confidence: 99%

Selective Anticancer Materials by Self-Assembly of Synthetic Amphiphiles Based on N-Acetylneuraminic Acid

Hwang

Kim

Park

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

N-Acetylneuraminic acid (Neu5Ac), one of the abundant types of sialic acid, is an emerging anticancer agent owing to its ability to target selectins in the plasma membrane of cancer cells. Considering the functionality of Neu5Ac, obtaining novel Neu5Ac-conjugated materials with a selective and an enhanced antitumor activity has remained a challenge. Herein, we report the supramolecular materials of three novel amphiphiles composed of Neu5Ac as a hydrophilic segment and pyrene or adamantane as a hydrophobic segment. The synthetic amphiphiles 1, 2, and 3 self-assembled into ribbons, vesicles, and irregular aggregates in an aqueous solution, respectively. Among the materials, vesicles of amphiphile 2 showed the most substantial selectivity toward cancer cells, followed by cell death due to the production of reactive oxygen species by the pyrene group. The dual advantage of Neu5Ac-selectivity and the pyrene-cytotoxicity of vesicles of amphiphile 2 can provide a strategy for effective anticancer materials.

show abstract

Section: Resultsmentioning

confidence: 99%

Selective Anticancer Materials by Self-Assembly of Synthetic Amphiphiles Based on N-Acetylneuraminic Acid

Hwang

Kim

Park

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Albumin and urea synthesis by HepG2 cells in Sw and GCT-Sw was evaluated on 1, 3, 5 and 7 days. HepG2 cells derived from hepatic carcinoma are often used in the toxicity models to analyze liver-specific functions in-vitro as it shares similar properties of hepatocytes 15,16 .…”

Section: Cell Proliferation Cck-8 Assay In Gctswmentioning

confidence: 99%

Untitled

2021

IJPSR

View full text Add to dashboard Cite

culture systems are indispensably used as in-vitro tools to improve our perception and understanding of cell biology, tissue engineering, tissue morphology, mechanisms of diseases, and drug action. However, the conventional 2D culture systems suffer from limitations such as alterations in cell morphology and lack of interactions between extracellular and cellular environments. As a result, three-dimensional (3D) cell cultures have received remarkable attention in studies such as drug discovery and development; the sandwich model being one of the unique cellular models widely used for analyzing tissue metabolism and functions. In this study, we devised a novel Gel Casting Tool (GCT) for in-vitro toxicity analysis. Liver cells need complex 3D micro-architecture to express tissue-specific functions and were suitable for this study. Human Carcinoma Cell line (HepG2 cells) with Gelatin methacrylamide (GelMA) hydrogel was used to check the efficacy of the GCT. The results showed that GCT Sandwiched HepG2 exhibited high cell viability, proliferation, and tissue-specific functions such as albumin and urea over 7 days. The major advantage of this culture model is the maximized availability of cells for observation under microscope and imaging cells in one plane. This culture system has potential application in in-vitro toxicity testing, early drug screening, and evaluation of scaffolds for tissue engineering. INTRODUCTION:Cell-based in vitro testing is the first and mandatory analysis that is followed in drug discovery and cytotoxicity evaluation. Cellbased in-vitro models represent a simple but very effective test.

show abstract

“…An important tool for determining the possible effects of these pollutants is the use of in vitro cell culture models. For instance, bioassays employing human hepatocarcinoma HepG2 cells have been convenient to estimate environmental and human health risk of sediment-bound chemical mixtures (Pinto et al, 2014 ), as these cells respond to high frequently found stressors in sediments, such as pesticides (Dehn et al, 2005 ; Medina-Díaz & Elizondo, 2005 ), PAHs (Ma et al, 2019 ), and PCBs (Wei et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Emerging contaminants and priority substances in marine sediments from Cartagena Bay and the Grand Marsh of Santa Marta (Ramsar site), Colombia

Caballero-Gallardo

Olívero-Verbel

Corada-Fernández

et al. 2021

Environ Monit Assess

View full text Add to dashboard Cite

Emerging pollutants and priority substances are of growing concern due to their toxicity potential to aquatic organisms and human health. However, few reports on this issue in marine ecosystems in general and, more specifically, on the Colombian Caribbean coast are available. The aim of this study was to detect these compounds in sediments from Cartagena Bay (CB) and in the Grand Marsh of Santa Marta, GMSM (Ramsar site), in order to determine how they related to in vitro cytotoxicity assays on HepG2 cells of sediment extracts. A total of thirty compounds were detected using GC–MS/MS in fifteen stations during both the rainy and the dry seasons. Sediments from CB had a wide range of different toxicants, with polycyclic aromatic hydrocarbons (PAHs) being the most prevalent (12 PAHs, 5.5–881.6 ng/g). Total PCBs ranged from < LOD to 18.6 ng/g, with PCB 138 being the most common detected congener. Residues of p , p ′-DDE, Chlorpyrifos and two organophosphate flame retardants, TEHP and ToTP, were found in most sampling locations. The UV filters 4MBC and homosalate were recurrently found in sediments, and the fragrance galaxolide appeared in all cases, with the greatest concentrations found on a touristic beach. In GMSM, with the exception of deltamethrin, all chemicals evaluated had lower average values than in CB. According to sediment quality guidelines, some sites in CB presented values of PAHs higher than the threshold effects level, while in the marsh, none of the stations exceeded it. HepG2 cells exposed to 1% sediment extracts presented reduced cell viability up to 26%. Cytotoxicity displayed a negative correlation with chlorpyrifos concentration. In short, these data suggest the bay and the marsh have specific contamination fingerprints related to anthropogenic interventions. This research highlights the need to further investigate the ecotoxicological implications of detected chemical stressors in these ecosystems. Supplementary Information The online version contains supplementary material available at 10.1007/s10661-021-09392-5.

show abstract

Effects of Pyrene on Human Liver HepG2 Cells: Cytotoxicity, Oxidative Stress, and Transcriptomic Changes in Xenobiotic Metabolizing Enzymes and Inflammatory Markers with Protection Trial Using Lycopene

Cited by 20 publications

References 36 publications

Selective Anticancer Materials by Self-Assembly of Synthetic Amphiphiles Based on N-Acetylneuraminic Acid

Selective Anticancer Materials by Self-Assembly of Synthetic Amphiphiles Based on N-Acetylneuraminic Acid

Untitled

Emerging contaminants and priority substances in marine sediments from Cartagena Bay and the Grand Marsh of Santa Marta (Ramsar site), Colombia

Contact Info

Product

Resources

About