Effects of pyrethroid insecticides on hepatic microsomal enzymes in rats

Krechniak, J; Wrześniowska, Krystyna

doi:10.1016/s0013-9351(05)80169-0

Cited by 20 publications

(6 citation statements)

References 6 publications

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“…Although, all treatment groups showed an increase in cytochrome P450 levels, only the results from PB‐treated rat cells were significantly different from controls ( P < 0.05, by anova followed by Dunnett's test). These results are in accordance with previous reports on the effects of PB, 3‐MC and deltamethrin on liver microsomal cytochrome P450 levels (Okey, 1990; Krechniak & Wrzesniowska, 1991; Dayal et al. , 1999).…”

Section: Resultssupporting

confidence: 93%

“…Organochlorines are well known inducers of cytochrome P450 activities, and persist in the environment for several years (Walker et al, 2001), and by accelerating the metabolism of the trypanocides in exposed animals during this time they may have contributed to the generation of trypanocide resistance. Their use is now environmentally unacceptable (Holmes, 1997), and they have been replaced by agents such as the synthetic pyrethroids (Walker et al, 2001), which although less persistent in the environment have also been shown to possess cytochrome P450 inducing properties (Krechniak & Wrzesniowska, 1991;Dayal et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Metabolism of isometamidium in hepatocytes isolated from control and inducer‐treated rats

Boibessot

Tettey

Skellern

et al. 2006

Vet Pharm & Therapeutics

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Little is known about the metabolism and mechanism of action of the trypanocide, isometamidium (ISM), the major drug used for prophylaxis of trypanosomiasis. We have investigated its metabolism and distribution in isolated rat hepatocytes using liquid chromatography-mass spectrometry and confocal laser scanning microscopy (CLSM). Two putative metabolites were formed, which were proposed to be a mono-acetyl derivative and an oxidized metabolite (SII). This is the first demonstration of the hepatic metabolism of ISM, as previous in vivo studies were hampered by dose-limiting toxicity and insensitive analytical methods. The intrinsic fluorescence of the drug enabled its intracellular uptake to be followed by CLSM. It is taken up rapidly into the nucleolus, nuclear membrane and endoplasmic reticulum within 5 min, and retained in the nucleus for at least 24 h. Persistent binding of ISM to cellular macromolecules may contribute to its prophylactic effect in vivo. Pretreatment of rats with 3-methylcholanthrene, phenobarbitone (PB) or the widely used pyrethroid pesticide, deltamethrin, resulted in an increase in metabolism of ISM to the proposed SII after 1 h incubation with hepatocytes. 3-methylcholanthrene was the most potent inducer, causing a maximal 19.5-fold induction of SII formation after exposure of hepatocytes to ISM for 1 h compared with formation by control hepatocytes. In comparison, at the 1 h timepoint deltamethrin pre-treatment caused a 10.2-fold induction, and PB only 8.2 fold.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Metabolism of isometamidium in hepatocytes isolated from control and inducer‐treated rats

Boibessot

Tettey

Skellern

et al. 2006

Vet Pharm & Therapeutics

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“…Our findings in case of GSH contents indicate that the level of GSH enhancement is pesticide concentration dependent. Krechniak and Wrzesniowska (1999) and Kale et al (1999) mentioned that the increase in reduced glutathione content in erythrocytes may probably be an initial adaptive response to increase oxidative stress. In comparison to the control group, the activity of glutathione S-transferase (GST) was higher in all pesticide treated rat erythrocytes or hepatocytes.…”

Section: Discussionmentioning

confidence: 99%

“…E-mail: dr.ahmedkhamis@yahoo.com. stress (Krechniak and Wrzesniowska, 1999;Kale et al, 1999). Major contributors to non-enzymatic protection against lipid peroxidation are vitamin E and vitamin C, well-known free radical scavengers (Kumar et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Pesticides-induced oxidative damage: Possible in vitro protection by antioxidants

Salama¹,

Omran²,

Osman³

2013

J. Toxicol. Environ. Health Sci.

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This study was designed to investigate the effect of selenium (Se) and a combination of vitamin E (VE) vitamin C (VC) on pesticides induced biochemical alterations in rat erythrocytes and hepatocytes in vitro.Vitamin E and C and selenium are potential antioxidants, known to be able to protect cells against oxidative damage. In vitro changes in antioxidant systems and protective role of selenium and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes and hepatocytes induced by atrazine (AT), dimethoate (DM), or endosulfan (ES) at three different levels of 10, 20, and 30 mM for each in rat were investigated. Levels of lipid peroxidation (nmoles MDA/mg protein), glutathione content (µmole GSH/mg protein) and glutathione peroxidase level (µmoles NADPH/min/mg protein) were determined in erythrocytes and hepatocytes following treatment. In comparison with the control, pesticides stimulated thiobarbituric acid reactive substances (TBARS) activity and glutathione peroxidase activity, enhanced the glutathione contents. Treatment with selenium and a combination of vitamin E and/or vitamin C potentially reduced the free radicals in erythrocytes or hepatocytes and ameliorated the oxidative stress induced by such pesticides. The results suggested that pesticides treatment increases in vitro lipid peroxidation, glutathione peroxidase level and glutathione content by increasing oxidative stress in erythrocytes and hepatocytes of rats and selenium and a combination of vitamin E and vitamin C can reduce this lipoperoxidative effect.

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“…Our recent data have demonstrated that cispermethrin is poorly metabolized by human liver fractions, although the trans-isomer is readily metabolized by both soluble and microsomal esterases and alcohol and aldehyde dehydrogenases [9]. CYP isoforms, mainly CYP2B1/2B2 and CYP1A1/2, are involved in deltamethrin hydroxylation [10] and are slightly induced by deltamethrin in the rat liver [10,11].…”

Section: Modification Ofmentioning

confidence: 97%

Abstracts of Posters Presented

Hodgson¹

2007

J Biochem & Molecular Tox

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Effects of pyrethroid insecticides on hepatic microsomal enzymes in rats

Cited by 20 publications

References 6 publications

Metabolism of isometamidium in hepatocytes isolated from control and inducer‐treated rats

Metabolism of isometamidium in hepatocytes isolated from control and inducer‐treated rats

Pesticides-induced oxidative damage: Possible in vitro protection by antioxidants

Abstracts of Posters Presented

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