Effects of quaternary benzo(c)phenanthridine alkaloids on growth performance, shedding of organisms, and gastrointestinal tract integrity in pigs inoculated with multidrug-resistant Salmonella spp

Robbins, Rebecca C.; Artuso-Ponte, Valeria; Moeser, Adam J.; Morrow, W. E. Morgan; Spears, J. W.; Gebreyes, Wondwossen A.

doi:10.2460/ajvr.74.12.1530

Cited by 17 publications

(11 citation statements)

References 23 publications

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“…The IQA formulation used is derived from Macleaya cordata , otherwise known as “plume poppy”, and is rich in IQA’s such as sanguinarine [ 21 ]. Extracts of Macleaya cordata have been shown to improve GIT health, as demonstrated by lowering diarrhea scores and Salmonella populations whilst promoting mucosal growth [ 17 , 20 , 32 ]. In a separate experiment, Macleaya cordata extracts reduced Salmonella , increased Lactobacillus populations, antioxidant capacity, parameters of immune function, small intestinal villus height, and growth performance [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Additional properties as a methyl donor could contribute to a wide range of biological reactions reducing oxidative stress and methylation pathways, improving efficiency in dairy [ 13 ], meat [ 14 ] and egg production [ 15 ] from heat-stressed livestock. Plant-derived isoquinoline alkaloids such as sanguinarine reduce stress and Salmonella shedding in transported pigs [ 16 , 17 ]. Isoquinoline alkaloids inhibit cellular Na + /K + ATPases, which are important contributors to thermogenesis [ 18 , 19 ] and therefore may provide an approach to mitigate heat stress.…”

Section: Introductionmentioning

confidence: 99%

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Betaine and Isoquinoline Alkaloids Protect against Heat Stress and Colonic Permeability in Growing Pigs

Shakeri

Suleria

et al. 2020

Antioxidants

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Heat stress (HS) compromises productivity of pork production, in part as a result of increased oxidative stress and inflammatory responses, particularly within the gastrointestinal tract. This study aimed to investigate whether plant-derived betaine and isoquinoline alkaloids could ameliorate HS in pigs. Fifty female Large White × Landrace grower pigs, which were acclimated to control (CON), control plus betaine (BET), or control plus isoquinoline alkaloids (IQA) diets for 14 days were then exposed to heat stress or thermoneutral condition. Both BET and IQA partially ameliorated increases in respiration rate (p = 0.013) and rectal temperature (p = 0.001) associated with HS conditions. Heat stress increased salivary cortisol concentrations and reduced plasma creatinine, lactate, and thyroid hormone concentrations. Heat stress increased colon FD4 permeability, which was reduced by IQA (p = 0.030). Heat stress increased inflammation in the jejunum and ileum, as indicated by elevated interleukin-1β (p = 0.022) in the jejunum and interleukin-1β (p = 0.004) and interleukin-8 (p = 0.001) in the ileum. No differences in plasma total antioxidant capacity (TAC) were observed with HS, but betaine increased plasma TAC compared to IQA. Dietary BET increased betaine concentrations in the jejunum, ileum (p < 0.001 for both), plasma, liver, kidney (p < 0.010 for all), urine (p = 0.002) and tended to be higher in muscle (p = 0.084). Betaine concentration was not influenced by HS, but it tended to be higher in plasma and accumulated in the liver. These data suggest that betaine and isoquinoline alkaloids supplementation ameliorated consequences of heat stress in grower pigs and protected against HS induced increases in colonic permeability.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Betaine and Isoquinoline Alkaloids Protect against Heat Stress and Colonic Permeability in Growing Pigs

Shakeri

Suleria

et al. 2020

Antioxidants

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“…Positive effects of sanguinarine on gut microbiota and integrity of intestinal mucosa have been reported (Liu et al, ; Robbins et al, ; Xue et al, ). Due to shifts in microbial metabolites in faeces, for example, significantly elevated ratio of acetic acid (in % of total, 52.3 ± 5.2 and 57.1 ± 4.5 for control and experimental group, resp., p = 0.042), or elevated total lactate values in faeces (in µmol/g, 20.1 ± 17.0 and 39.9 ± 51.3 for control and experimental group, resp.)…”

Section: Discussionmentioning

confidence: 99%

Effects of isoquinoline alkaloids from Macleaya cordata on physiological, immunological and inflammatory parameters in healthy beagles

Faehnrich

Pastor

Heide

et al. 2018

Animal Physiology Nutrition

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The aim of the study was to investigate nutritional, physiological and immunological effects of a plant-derived blend of isoquinoline alkaloids (Sangrovit® Extra) in healthy dogs. Two groups of healthy, adult beagles (N = 10) were tested in a cross-over experiment, lasting two consecutive three-week periods. The experimental group received 1.2 g additive/kg feed, according to the recommendation of 10-20 mg/kg live weight per day. The control group received the same feed without additive. Complete blood count, immunological parameters and amino acid concentrations in serum were assessed. Faeces were analysed for short-chain fatty acids, lactate and ammonium; moreover, their quantity and consistency were determined. Neither feed intake, total apparent nutrient digestibility (crude protein and fat, organic matter, sodium, potassium) were affected by intake of the product. Lymphocyte and monocyte counts were slightly increased in both groups. Elevation was not treatment dependant. IgA, IgG, haptoglobin in serum and flow cytometric phenotyping of peripheral lymphocytes were not affected by alkaloids supplementation. Numerically greater methionine concentrations in blood serum occurred in the experimental group (p = 0.182). Quantity and consistency of faeces and ammonium concentration in faeces were not affected by the additive. Faecal concentrations of short-chain organic acids differed between groups (acetic acid, % of total SCFA: control group 52.3 ± 5.2 vs. experimental group 57.1 ± 4.5, p = 0.042), lactate concentrations (d-, l-and total) did not. Due to the shift of SCFA proportions in faeces, an effect of isoquinoline alkaloids (IQs) on the metabolic activity of intestinal microbiota is probable.In conclusion, the addition of IQs in the given dose was well tolerated and did not have adverse effects in healthy dogs. K E Y W O R D Santi-inflammatory, blood count, digestibility, dog, isoquinoline alkaloid, Macleaya cordata

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“…1,5 On the other hand, formulations containing S are administered as supplements to livestock diets, since it promotes better nutrient absorption and digestion. 3,5 There also studies showing good antimicrobial activity of C against multi-drug resistant, methicillinresistant Staphylococcus aureus strains (mdr-MRSA). 1,5 Moreover, Bn, C and S might play a defensive role in plants that produce them given their antimicrobial, anti-herbivory and allelopathic properties.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] Such properties have been associated to the presence of benzylisoquinoline alkaloids (BIA's), which are distributed through the entire plant 4 . The main Argemone alkaloids are berberine (Bn), chelerythrine (C) and sanguinarine (S), which belong to the protoberberine (Bn) and benzophenanthridine (C and S) types.…”

Section: Introductionmentioning

confidence: 99%

HPLC-DAD determination of berberine, chelerythrine and sanguinarine in the mexican prickly poppy (Argemone mexicana L. Papaveraceae), a medicinal plant

Xool-Tamayo¹,

Graniel-Sabido²,

Mirón-López³

et al. 2017

Quim. Nova

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A sensitive, simple, rapid and reliable HPLC-DAD method for the analysis of the benzylisoquinoline alkaloids (BIA's) content in Argemone mexicana (Papaveraceae) is presented. This method allows the simultaneous separation and quantitation of berberine (Bn), chelerythrine (C) and sanguinarine (S) in extracts from A. mexicana tissues, reducing time of analysis in comparison to previous reports. Alkaloids were separated on a C18 Hypersil Gold column using an acetonitrile gradient (20 to 70%) in 1% acetic acid in water. Alkaloids were identified based on retention times and UV spectra and quantified at 254 nm. Linearity between 0.5-20 µg mL -1 was observed for Bn, C and S, with limits of detection (LOD) and quantitation (LOQ) of 0.11 and 0.33 for Bn, 0.10 and 0.30 for C and 0.05 and 0.15 for S, respectively. Maximal intra-and inter-day variation values were < 0.49% in all cases, with alkaloids' recoveries higher than 95%. System suitability tests (SST), including resolution (Rs), retention factor (K'), selectivity (a), tailing factor and number of theoretical plates were performed according to the United States Pharmacopeia (USP), fulfilling recommended values. The method proved to be efficient and reproducible when analyzing different tissues of field-collected A. mexicana plants.

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Effects of quaternary benzo(c)phenanthridine alkaloids on growth performance, shedding of organisms, and gastrointestinal tract integrity in pigs inoculated with multidrug-resistant Salmonella spp

Cited by 17 publications

References 23 publications

Betaine and Isoquinoline Alkaloids Protect against Heat Stress and Colonic Permeability in Growing Pigs

Betaine and Isoquinoline Alkaloids Protect against Heat Stress and Colonic Permeability in Growing Pigs

Effects of isoquinoline alkaloids from Macleaya cordata on physiological, immunological and inflammatory parameters in healthy beagles

HPLC-DAD determination of berberine, chelerythrine and sanguinarine in the mexican prickly poppy (Argemone mexicana L. Papaveraceae), a medicinal plant

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