Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro

Deng, Xiaohui; Song, Haiyan; Yingfeng, Zhou; Yuan, Guoyan; Fang, Zheng

doi:10.3892/etm.2013.1285

Cited by 111 publications

(82 citation statements)

References 13 publications

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“…In other studies, Quercetin inhibited the growth of breast cancer cells. The mechanism behind this effect could be the induction of apoptosis by triggering G0/G1 phase arrest (Deng et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Despite the development of surgical techniques and chemotherapy, relapses occur very often especially in advanced cases (Deng et al, 2013). Therefore, the development of new antitumor substances is of paramount importance in the ongoing war against cancer.…”

Section: Introductionmentioning

confidence: 99%

“…Breast cancer is one of the most common malignancies and it has a serious impact on female health (Deng et al, 2013). Worldwide, it is the most frequently diagnosed cancer and the leading cause of cancer death among females (Torre et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Trolox and Quercetin Combinations on Human Ovarian Cancer Cell Line A2780 and Human Breast Cancer Cell Line T47D-KBluc

et al. 2015

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Our aim was to investigate whether combining two powerful antioxidants such as Trolox and Quercetin could enhance their toxicity against breast and ovarian cancer cells. Human ovarian carcinoma cells from the A2780 line and human mammary gland carcinoma cells belonging to the T47D-KBluc line were incubated in RPMI-1640 supplemented with 50 µM Trolox and various Quercetin concentrations (25 µM, 50 µM and 100 µM). After 24 hours, several test were conducted: cells were stained with trypan blue and the number of viable versus dead cells was counted; cells were stained with Hoechst 33258 and propidium iodide to investigate the incidence of apoptosis and necrosis; cell activity and proliferation was assessed using the MTT test. In the A2780 line 50 µM Trolox + 25µM Quercetin increased the number of apoptotic cells and enzyme activity in these cells, without influencing viability. On the other hand, in the T47D-KBluc line the 50 µM Trolox + 100 µM combination enhanced necrosis and thus reduced the percentages of metabolically active cells. Together with this concentration, a mix made of 50 µM Trolox and 25 µM Quercetin also reduced cell viability. Our research indicates that in combination with Trolox, low concentrations of Quercetin induce apoptosis in human ovarian cancer cells, while high concentrations induce necrosis in human breast cancer cells. Quercetin concentrations influence this response significantly. Combining Quercetin with Trolox can enhance their toxicity against breast and ovarian cancer cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Trolox and Quercetin Combinations on Human Ovarian Cancer Cell Line A2780 and Human Breast Cancer Cell Line T47D-KBluc

et al. 2015

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“…Quercetin treatment led to variations in survivin mRNA expressions and G 0 /G 1 phase cell cycle arrest in MCF-7 cells. All these effects depended on concentration and exposure time 84 . An in vivo experiment on the synergistic effect of quercetin with doxorubicin injected in mice with breast cancer was carried out.…”

Section: Quercetinmentioning

confidence: 99%

“…It was concluded that quercetin enhanced the anti-cancer activity of 5GG in MDA-MB-231 cells 42 . Deng et al 84 described the effects of quercetin against MCF-7 cells in laboratory conditions. Quercetin treatment led to variations in survivin mRNA expressions and G 0 /G 1 phase cell cycle arrest in MCF-7 cells.…”

Section: Quercetinmentioning

confidence: 99%

An Insight into the Putative Role of Victuals Like Honey and its Polyphenols in Breast Cancer

et al. 2017

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Diet plays a crucial role in cancer advancement as well as prevention. Breast cancer is the second leading cause of cancer death among women. Recent research links breast cancer with diet and some evidence for the preventive effect of diet against breast cancer was also documented. The growth of cancer cells is influenced by natural sweetener honey and its multitude of phenolic phytochemical components. Honey has been used medicinally by ancient Greeks and Egyptians and also traditionally exploited in Ayurveda and Chinese medicine. In this paper, the anti-cancer properties of honey and its phytochemical's action against breast cancer have been summarized. They result in apoptosis by enhancing reactive oxygen species level, activating mitochondrial pathway, initiation of pro-apoptotic and anti-apoptotic proteins, induction of p53 pathway that finally cause DNA fragmentation. However, there is a necessity for more proteomic and genetic-based experiments to understand its molecular mechanism to promote honey and its phenolic markers as plausible candidates for breast cancer treatment. Further, there is a need for quality check of honey available in the market, which warrants significant investigation by researchers in the food industry to ensure their attributes.

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Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

Solaimuthu

Mukherjee

Das

et al. 2017

Cell Biochemistry & Function

154

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Epidermal growth factor plays a major role in breast cancer cell proliferation, survival, and metastasis. Quercetin, a bioactive flavonoid, is shown to exhibit anticarcinogenic effects against various cancers including breast cancer. Hence, the present study was designed to evaluate the effects of gold nanoparticles-conjugated quercetin (AuNPs-Qu-5) in MCF-7 and MDA-MB-231 breast cancer cell lines. Borohydride reduced AuNPs were synthesized and conjugated with quercetin to yield AuNPs-Qu-5. Both were thoroughly characterized by several physicochemical techniques, and their cytotoxic effects were assessed by MTT assay. Apoptotic studies such as DAPI, AO/EtBr dual staining, and annexin V-FITC staining were performed. AuNPs and AuNPs-Qu-5 were spherical with crystalline nature, and the size of particles range from 3.0 to 4.5 nm. AuNPs-Qu-5 exhibited lower IC value compared to free Qu. There was a considerable increase in apoptotic population with increased nuclear condensation seen upon treatment with AuNPs-Qu-5. To delineate the molecular mechanism behind its apoptotic role, we analysed the proteins involved in apoptosis and epidermal growth factor receptor (EGFR)-mediated PI3K/Akt/GSK-3β signalling by immunoblotting and immunocytochemistry. The pro-apoptotic proteins (Bax, Caspase-3) were found to be up regulated and anti-apoptotic protein (Bcl-2) was down regulated on treatment with AuNPs-Qu-5. Additionally, AuNPs-Qu-5 treatment inhibited the EGFR and its downstream signalling molecules PI3K/Akt/mTOR/GSK-3β. In conclusion, administration of AuNPs-Qu-5 in breast cancer cell lines curtails cell proliferation through induction of apoptosis and also suppresses EGFR signalling. AuNPs-Qu-5 is more potent than free quercetin in causing cancer cell death, and hence, this could be a potential drug delivery system in breast cancer therapy.

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Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro

Cited by 111 publications

References 13 publications

Influence of Trolox and Quercetin Combinations on Human Ovarian Cancer Cell Line A2780 and Human Breast Cancer Cell Line T47D-KBluc

Influence of Trolox and Quercetin Combinations on Human Ovarian Cancer Cell Line A2780 and Human Breast Cancer Cell Line T47D-KBluc

An Insight into the Putative Role of Victuals Like Honey and its Polyphenols in Breast Cancer

Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

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