Effects of quorum‐sensing inhibition on experimental periodontitis induced by mixed infection in mice

Abstract

This study aimed to verify, in in vivo settings, whether quorum-sensing inhibition molecules could attenuate alveolar bone loss induced by Porphyromonas gingivalis/Fusobacterium nucleatum co-infection and reduce the bacterial colonization of periodontal tissues. In BALB/c mice, periodontitis was induced through oral inoculation with P. gingivalis and F. nucleatum six times during a 42-d period. Quorum sensing inhibitors (a furanone compound and D-ribose) were administered simultaneously with bacterial infection. Linear and volumetric modifications of interproximal alveolar bone levels were compared between groups using micro-computed tomography. Total bacteria, and P. gingivalis and F. nucleatum DNA in periodontal tissues, were quantified using real-time PCR. Radiographic linear measurements demonstrated a significant reduction of alveolar bone loss, of approximately 40%, in mice treated with quorum sensing inhibitors when compared with the co-infection group. This was confirmed by a significant increase of residual bone volume in the test group. While total bacterial genes in the treatment group significantly decreased by 93% in periodontal tissue samples when quorum sensing inhibitors were administered, no significant differences of P. gingivalis DNA were found. Quorum sensing inhibitors reduced periodontal breakdown and bacterial infection in periodontal tissues after co-infection with P. gingivalis and F. nucleatum.

Microbiome in maintained periodontitis and its shift over a single maintenance interval of 3 months

“…Our study demonstrated an intricate antagonistic relationship between two distinct groups of bacterial organisms—beneficial taxa and pathogenic taxa, which is in agreement with previous studies about periodontitis (Shi et al, ). Adhesins, nutrition and metabolism might count for the synergistic relationship, and lactose‐inhibitable congregations and quorum sensing might count for the antagonistic relationship among genera (Ben Amara et al, ; Kolenbrander & London, ; Polak, Shapira, Weiss, & Houri‐Haddad, ; Rovai & Holzhausen, ). The underlying mechanisms must be investigated in future studies.…”

Effect of D-Ribose on Fusobacterium Nucleatum Planktonic Proliferation and Biofilm Maturation

“…Studies examining the clinical effectiveness of D-ribose have also shown that it can reduce periodontal damage. For example, Ben Amara et al (29) found in their in-vivo study that the presence of 50mM D-ribose may reduce periodontal damage. In the same study it was also found that the total bacterial count was significantly reduced in the group treated with D-ribose.…”

Sustained‐release delivery of antimicrobial drugs for the treatment of periodontal diseases: Fantasy or already reality?

“…Several drugs have been shown to affect crosstalk (quorum sensing) among periopathogenic bacteria. For example, the use of a quorum‐sensing inhibitor against biofilm formation by periopathogenic bacteria and quorum‐sensing inhibitor resulted in a reduction of experimental periodontitis in mice . Several sustained‐release delivery systems containing potential quorum‐sensing inhibitors have been developed and tested in vitro against different microbes in the oral cavity .…”