Effects of R219K polymorphism of ATP-binding cassette transporter 1 gene on serum lipids ratios induced by a high-carbohydrate and low-fat diet in healthy youth

Abstract

BackgroundDiets are the important players in regulating plasma lipid profiles. And the R219K polymorphism at the gene of ATP-binding cassette transporter 1(ABCA1) was reported to be associated with the profiles. However, no efforts have been made to investigate the changes of lipid profiles after a high-carbohydrate and low-fat diet in different subjects with different genotypes of this polymorphism. This study was to evaluate the effects of ABCA1 R219K polymorphism on serum lipid and apolipoprotein (apo) ratios induced by a high-carbohydrate/low-fat (high-CHO) diet. After a washout diet of 54.1% carbohydrate for 7 days, 56 healthy young subjects (22.89 ± 1.80 years old) were given a high-CHO diet of 70.1% carbohydrate for 6 days. Height, weight, waist circumference, hip circumference, glucose (Glu), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apoA-1 and apoB-100 were measured on the 1st, 8th and 14th days of this study. Body mass index (BMI), waist-to-hip ratios (WHR), log(TG/HDL-C), TC/HDL-C, LDL-C/HDL-C and apoA-1/apoB-100 were calculated. ABCA1 R219K was analyzed by a PCR-RFLP method.Results The results indicate that the male subjects of all the genotypes had higher WHR than their female counterparts on the 1st, 8th and 14th days of this study. The male K carriers had higher log(TG/HDL-C) and TC/HDL-C than the female carriers on the 1st and 14th days, and higher LDL-C/HDL-C on the 14th day. When compared with that on the 8th day, TC/HDL-C was decreased regardless of the genotypes and genders on the 14th day. Log(TG/HDL-C) was increased in the males with the RR genotype and the female K carriers. Lowered BMI, Glu and LDL-C/HDL-C were found in the male K carriers, but only lowered BMI in the female K carriers and only lowered LDL-C/HDL-C in the females with the RR genotype.Conclusions These results suggest that ABCA1 R219K polymorphism is associated differently in males and females with elevated log(TG/HDL-C) and decreased LDL-C/HDL-C induced by the high-CHO diet.

Common Genetic Variations Involved in the Inter-Individual Variability of Circulating Cholesterol Concentrations in Response to Diets: A Narrative Review of Recent Evidence

“…This variant is restricted to the Americas, where its presence explains up to 4% of the variation in HDL-C concentrations [ 40 ]. In an intervention study, 56 healthy Chinese adults received a washout diet where carbohydrate contributed 54% of energy for 7 d, followed by a high-carbohydrate/low-fat diet where carbohydrate and fat contributed 70% and 14% of energy, respectively, for 6 d [ 14 ]. Males carrying the A (published as K) allele and females carrying the GG (published as RR) genotype at rs2230806 in ABCA1 (G1051A; published as R219K, a missense variant, as defined by the amino acid change) displayed lower LDL-C/HDL-C concentration ratios (1.08 ± 0.27 vs. 1.48 ± 0.64 in males, and 1.01 ± 0.18 vs. 1.37 ± 0.33 in females) after the high-carbohydrate/low-fat diet [ 14 ].…”

Biological Research marks its 50th year by joining BioMed Central

“…Among the first articles in volume 47 of Biological Research, published by BioMed Central as open access manuscripts, we include a variety of papers covering different areas of experimental biology. They include manuscripts on biomedicine (such as human cancer genetics (Ramalho et al, Brazil) [ 3 ]; genetic polymorphisms associated with human nutrition, (Fang et al, China) [ 4 ]; animal physiology (oxidative stress and nutrition, Otunola et al, South Africa) [ 5 ]; molecular biology (new molecular biotechnology tools to attack spider toxins, Sapag et al, Chile) [ 6 ]; plant biology (antinflammatory effect of a native medicinal plant; Morales et al, Chile) [ 7 ]; and animal experimentation (cardiac function in rats, Zheng et al, China) [ 8 ].…”

Association between the ABCA1 (R219K) polymorphism and lipid profiles: a meta-analysis

“…RR vs. RK + KK; Table 1 ). Previous studies 63 , 76 , 77 reported that the RR genotype had a higher level of TG than K carriers (SMD = 0.18 mmol/L, 95%CI 0.06 to 0.30, z = 3.01, P < 0.01). For the LDLC level, some studies 65 , 78 and meta-analyses 28 reported the association between R219K and LDLC level, which disappeared when studies with the outliers were removed.…”