Effects of Rabbit Sacculus Rotundus Antimicrobial Peptides on the Intestinal Mucosal Immunity in Chickens

Liu, T.; She, Ruiping; Wang, K.; Bao, Huihui; Zhang, Y.; Luo, Dan; Hu, Yuanliang; Ding, Ye; Wang, D.; Kong, Peng

doi:10.3382/ps.2007-00353

Cited by 61 publications

(40 citation statements)

References 19 publications

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“…Intestine morphology may be mediated via alteration of intestinal microflora. It was shown that, the digestive function of the small intestine is closely related to mucosal architecture and villi structure (Liu et al, 2008). Few reports are available that have compared the effect of probiotic types on broiler intestine morphology.…”

Section: Discussionmentioning

confidence: 99%

The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers

Aliakbarpour

Chamani

Rahimi

et al. 2012

Asian Australas. J. Anim. Sci

156

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ABSTRACT:The aim of the present study was to evaluate the effect of commercial monostrain and multistrain probiotics in diets on growth performance, intestinal morphology and mucin gene (MUC2) expression in broiler chicks. Three hundred seventy-eight 1-d-old male Arian broiler chicks were allocated in 3 experimental groups for 6 wk. The birds were fed on a corn-soybean based diet and depending on the addition were labeled as follows: control-unsupplemented (C), birds supplemented with Bacillus subtilis (BS) and lactic acid bacteria (LAB) based probiotics. Each treatment had 6 replicates of 21 broilers each. Treatment effects on body weight, feed intake, feed conversion ratio and biomarkers such as intestinal goblet cell density, villus length, villus width, and mucin gene expression were determined. Total feed intake did not differ significantly between control birds and those fed a diet with probiotics (p>0.05). However, significant differences in growth performance were found. Final body weight at 42 d of age was higher in birds fed a diet with probiotics compared to those fed a diet without probiotic (p<0.05). Inclusion of Bacillus subtilis based probiotic in the diets also significantly affected feed conversion rate (FCR) compared with control birds (p<0.05). No differences in growth performance were observed in birds fed different types of probiotic supplemented diets. Inclusion of lactic acid bacteria based probiotic in the diets significantly increased goblet cell number and villus length (p<0.05). Furthermore, diets with Bacillus subtilis based probiotics significantly increased gene expression (p<0.05), with higher intestinal MUC2 mRNA in birds fed diet with probiotics compared to those fed the control diet. In BS and LAB probiotic fed chicks, higher growth performance may be related to higher expression of the MUC2 gene in goblet cells and/or morphological change of small intestinal tract. The higher synthesis of the mucin gene after probiotic administration may positively affect bacterial interactions in the intestinal digestive tract, intestinal mucosal cell proliferation and consequently efficient nutrient absorption.

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Section: Discussionmentioning

confidence: 99%

The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers

Aliakbarpour

Chamani

Rahimi

et al. 2012

Asian Australas. J. Anim. Sci

156

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“…The histological sections (4 µm) were immersed in citrate buffer solution (0.01 M, pH6.0) and heated at 120°C in autoclaved sterilizer for 10 min, naturally cooled for 30 min, and then immersed in 3% aqueous hydrogen peroxide (H 2 O 2 ) for endogenous peroxidase ablation at room temperature for 30 min. The following steps were executed in a moist chamber and this procedure has been exclusively described by Liu et al [51]. Briefly, the histological sections were washed in PBS, quenched with blocking buffer (Zymed Laboratories Inc., San Diego, USA).…”

Section: Methodsmentioning

confidence: 99%

Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect

Ding

Zou

et al. 2011

PLoS ONE

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To explore the effects of long-term weightlessness on the renal tissue, we used the two months tail suspension model to simulate microgravity and investigated the simulated microgravity on the renal morphological damages and related molecular mechanisms. The microscopic examination of tissue structure and ultrastructure was carried out for histopathological changes of renal tissue morphology. The immunohistochemistry, real-time PCR and Western blot were performed to explore the molecular mechanisms associated the observations. Hematoxylin and eosin (HE) staining showed severe pathological kidney lesions including glomerular atrophy, degeneration and necrosis of renal tubular epithelial cells in two months tail-suspended rats. Ultrastructural studies of the renal tubular epithelial cells demonstrated that basal laminas of renal tubules were rough and incrassate with mitochondria swelling and vacuolation. Cell apoptosis in kidney monitored by the expression of Bax/Bcl-2 and caspase-3 accompanied these pathological damages caused by long-term microgravity. Analysis of the HSP70 protein expression illustrated that overexpression of HSP70 might play a crucial role in inducing those pathological damages. Glucose regulated protein 78 (GRP78), one of the endoplasmic reticulum (ER) chaperones, was up-regulated significantly in the kidney of tail suspension rat, which implied that ER-stress was associated with apoptosis. Furthermore, CHOP and caspase-12 pathways were activated in ER-stress induced apoptosis. Resistance training not only reduced kidney cell apoptosis and expression of HSP70 protein, it also can attenuate the kidney impairment imposed by weightlessness. The appropriate optimization might be needed for the long term application for space exploration.

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“…may contribute to the growth of the embryos as reported previously (Liu et al, 2008;Bao et al, 2009;Wang et al, 2009). To further investigate the effect of SIAMP on the inhibition of IBV, we measured the agglutination titer of allantoic fluid of the chick embryos.…”

Section: Swine Intestine Antimicrobial Peptidesmentioning

confidence: 96%

Swine intestine antimicrobial peptides inhibit infectious bronchitis virus infectivity in chick embryos

et al. 2010

Self Cite

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Infectious bronchitis virus (IBV), the causative agent of infectious bronchitis, results in respiratory disease, nephritis, and poor egg production and quality in chicken. Antimicrobial peptides possess potent antimicrobial activities and are regarded as promising therapeutic alternatives in the fight against microorganisms. To assess the in vitro antiviral activity of swine intestine antimicrobial peptides (SIAMP) against IBV, 45 chick embryos were randomly assigned into 3 groups, 15 for each group. Embryos in group 1 were inoculated with IBV. Group 2 was inoculated with SIAMP-IBV intermixture. Group 3 was used as a control and inoculated with sterile PBS. Allantoic fluid was collected for hemagglutination titer assay. In addition, weight gain, mortality, and pathological changes for each group were recorded. The results showed that no distinct pathological changes were found in chick embryos after they were inoculated with SIAMP-IBV intermixture and the mortality was reduced remarkably compared with the IBV-infected group. Weight gain of embryos in the SIAMP-IBV intermixture group was significantly higher than the IBV-infected embryos (P < 0.01), which was also higher than the control group (P < 0.05). Furthermore, the hemagglutination titer in the SIAMP-IBV group was significantly lower than that in the IBV-infected group (P < 0.01). These results indicated that SIAMP can inhibit virus replication and reduce tissue injury caused by IBV.

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Effects of Rabbit Sacculus Rotundus Antimicrobial Peptides on the Intestinal Mucosal Immunity in Chickens

Cited by 61 publications

References 19 publications

The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers

The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers

Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect

Swine intestine antimicrobial peptides inhibit infectious bronchitis virus infectivity in chick embryos

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