Effects of radiation quality and dose rate on radiation-induced nucleoplasmic bridges in human peripheral blood lymphocytes

Hua, Zhao; Cai, Tian-Jing; Lü, Xue; Tian, Mei; Liu, Qingjie

doi:10.1016/j.mrgentox.2021.503321

Cited by 1 publication

(1 citation statement)

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“…Micronuclei are known as a biomarker of DNA repair ability and chromosomal instability and are detected DNA damage following exposure to genotoxic agents (Dhillon et al, 2011). Therefore, MN formation is associated with chromosomal instability observed in many types of cancer and there is a linear correlation between high MN frequency in human peripheral lymphocytes and cancer incidence (Bonassi et al, 2011; Zhao et al, 2021). In this study, the MN test revealed no significant change in the frequency of micronucleated cells treated for amygdalin alone.…”

Section: Discussionmentioning

confidence: 99%

A study on Amygdalin's genotoxicological safety and modulatory activity in human peripheral lymphocytes in vitro

Erikel

Yüzbaşıoğlu

Ünal

2023

Environ and Mol Mutagen

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Amygdalin (AMY), a plant secondary metabolite containing nitrile, is a major component of the seeds of Rosaceae family plants. It is known that this compound has many pharmacological activities such as cancer prevention, antipyretic, and cough suppressant. In this study, the genotoxic and modulatory effects of amygdalin were assessed by chromosomal aberration (CA), sister chromatid exchange (SCE), and cytokinesis‐block micronucleus assay (CBMN) assays using human peripheral lymphocytes (HPLs) in the absence and presence of metabolic activator (S9 mix). Lymphocytes were exposed to various concentrations of amygdalin (0.86, 1.72, 3.43, 6.86, and 13.75 μg/mL) alone and in combination with mitomycin‐C (MMC, 0.20 μg/mL) or cyclophosphamide (CP, 12 μg/mL). The mitotic index (MI), replication index (RI), cytokinesis‐block proliferation index (CBPI), and cytostasis were also evaluated to determine cytotoxicity. Amygdalin alone did not exhibit genotoxic and cytotoxic effects at all the tested concentrations both in the absence and presence of the S9 mix. In contrast, amygdalin significantly reduced the frequencies of CA (especially at 48 h treatments), SCE, and MN (except 0.86 μg/mL in pre‐ and simultaneous treatment) induced by MMC in all the tested concentrations and treatment protocols. It has also considerably decreased CP‐induced CA and SCE frequencies at all the concentrations (except 0.86 μg/mL) in simultaneous treatment. This study demonstrated that amygdalin alone was not genotoxic, on the contrary, it has revealed modulatory effects against chemotherapy agents that induced genomic damage in human lymphocytes, suggesting its chemopreventive potential.

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Section: Discussionmentioning

confidence: 99%