Effects of radioactive 125I on apoptosis of HGC-27 gastric cancer cells

Abstract

Effects of radioactive 125I particles at different doses on apoptosis of HGC-27 gastric cancer cells were investigated. HGC-27 gastric cancer cell suspension was used to establish a tumor-bearing mouse model. The model was reared for approximately 3 weeks and then divided into the control group (implanted with blank particles), the low dose group (implanted with 1.48×10−7 Bq 125I particles), the medium dose group (implanted with 2.22×10−7 Bq 125I particles) and the high dose group (implanted with 2.96×10−7 Bq 125I particles) (n=15 per group). Six nude mice were randomly sacrificed to collect the tumor tissue and measure tumor volume and mass. TUNEL (TdT-mediated dUTP nick-end labeling) was used for detecting apoptosis of tumor cells, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for detecting the relative expression of Bax, caspase-3 and caspase-8. On the 28th day after implantation, the apoptotic rate in the low, medium and high dose groups was significantly higher than that in the control group, which in the medium and high dose groups was significantly lower than that in the low dose group (P<0.05). On the 28th day after implantation, the relative expression of Bax, caspase-3 and caspase-8 mRNA in the control group was significantly lower than that in the low, medium and high dose groups (P<0.05), which in the low dose group was significantly higher than that in the medium and high dose groups (P<0.05). 125I particles can inhibit the growth of HGC-27 gastric cancer cell transplants and promote the expression of Bax, caspase-3 and caspase-8 mRNA in the tumor tissue. Low-dose 125I particles are significantly more effective than medium- or high-dose 125I particles.

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“… 33 125I particles also can inhibit the growth of HGC-27 gastric cancer cell transplants and promote the expression of Bax, caspase-3, and caspase-8 mRNA in the tumor tissue. 34 These studies had confirmed that 125 I seed brachytherapy could inhibit the proliferation and promote the apoptosis of cells. 35 So when 125 I seeds are implanted in the lesions of NSCLC, persistent low-dose irradiation could affect the different stages of cell cycle, which could continue to kill tumor cells.…”

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“…erefore, (125)I particle implantation can produce a high dose at the site, while the radiation dose of surrounding normal tissues is low, with highly conformal radiation [13][14][15]. Zhao et al found that (125)I seed implantation based on CT-guided 3D template-assisted technique can Evidence-Based Complementary and Alternative Medicine effectively alleviate the tumor and improve the survival rate in patients with refractory malignant tumors [16].…”