Effects of Reactive Oxygen Species on Cell Proliferation and Death in HeLa Cells and Its MDR1-Overexpressing Derivative Cell Line.

Liu, Yuwen; Sakaeda, Toshiyuki; Takara, Kohji; Nakamura, Tsutomu; Ohmoto, Nobuko; Komoto, Chiho; Kobayashi, Hironao; Yagami, Tatsurou; Okamura, Noboru; Okumura, Katsuhiko

doi:10.1248/bpb.26.278

Cited by 21 publications

(14 citation statements)

References 15 publications

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“…However, it is still hard to find an appealing explanation for why MCF-7/ADM cells had higher levels of iROS than MCF-7 cells. One possible reason may be that the iROS level varies in different cell origins, types, cultural methods, and so forth, and so does the cellular response to ROS, as it is reported that upregulation of P-pg by reduction of ROS levels was only observed in HepG2 cells but not in MCF-7, A549, A431, HeLa, and Hvr100-6 cell lines [42, 43]. …”

Section: Discussionmentioning

confidence: 99%

Long‐Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF‐7 Cells

Cen

Zhang

Liu

et al. 2016

Oxidative Medicine and Cellular Longevity

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Reactive oxygen species (ROS) play an important role in multidrug resistance (MDR). This study aimed to investigate the effects of long-term ROS alteration on MDR in MCF-7 cells and to explore its underlying mechanism. Our study showed both long-term treatments of H2O2 and glutathione (GSH) led to MDR with suppressed iROS levels in MCF-7 cells. Moreover, the MDR cells induced by 0.1 μM H2O2 treatment for 20 weeks (MCF-7/ROS cells) had a higher viability and proliferative ability than the control MCF-7 cells. MCF-7/ROS cells also showed higher activity or content of intracellular antioxidants like glutathione peroxidase (GPx), GSH, superoxide dismutase (SOD), and catalase (CAT). Importantly, MCF-7/ROS cells were characterized by overexpression of MDR-related protein 1 (MRP1) and P-glycoprotein (P-gp), as well as their regulators NF-E2-related factor 2 (Nrf2), hypoxia-inducible factor 1 (HIF-1α), and the activation of PI3K/Akt pathway in upstream. Moreover, several typical MDR mediators, including glutathione S-transferase-π (GST-π) and c-Myc and Protein Kinase Cα (PKCα), were also found to be upregulated in MCF-7/ROS cells. Collectively, our results suggest that ROS may be critical in the generation of MDR, which may provide new insights into understanding of mechanisms of MDR.

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Section: Discussionmentioning

confidence: 99%

Long‐Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF‐7 Cells

Cen

Zhang

Liu

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…Moreover, in the literature, it has been found that reactive oxygen species (ROS) production transiently increases with time upon labelling of cells with iron oxide nanoparticles; [45] this leads to a transiently improved cellular growth rate and changes in the cell cycle. [46] Ultimately, these alterations might influence the level of ML uptake. The steady decrease of the iron concentration could also be explained by a possible leaching of the MLs.…”

Section: Discussionmentioning

confidence: 99%

Optimal Conditions for Labelling of 3T3 Fibroblasts with Magnetoliposomes without Affecting Cellular Viability

2007

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A comparative study that deals with the internalisation of different types of magnetoliposomes (MLs) by 3T3 fibroblasts revealed that cationic MLs proved to be superior to neutral and anionic ones. Internalisation was visualised both by optical light and transmission electron microscopy. The latter showed that the cationic MLs ultimately ended up in lysosomal structures. The effect of increasing 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) concentrations in the cationic ML coat has been elucidated. High uptake efficiency was only achieved with MLs that carry a high DOTAP payload. However, these structures also demonstrated toxic effects. The use of the saturated distearoyl analogue (DSTAP) at identical concentrations led to improved uptake efficiency and lower toxicity. By using iron-oxide-free vesicles, it was shown that the toxicity was due to lipid bilayer constituents and not the iron oxide. In conclusion, the use of DMPC-DSTAP (96.67:3.33; molar ratio) MLs results in an extremely high labelling of 3T3 fibroblasts with iron oxides (47.66 pg Fe per cell) without evoking any influence on cell viability.

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“…The effects of ABM extract on the growth of HeLa, HeLa/TXL and HeLa/CDDP cells were evaluated with the WST-1 assay (Iida et al, 2001;Liu et al, 2003;Takara et al, 2002;Takara et al, 2005). Cells were seeded into 96-well plates in 100 µl of culture medium without any drugs.…”

Section: Wst-1 Colorimetric Assaymentioning

confidence: 99%

“…The cytotoxicity of chemotherapeutic agents in HeLa, HeLa/TXL and HeLa/CDDP cells was also evaluated with the WST-1 assay (Iida et al, 2001;Liu et al, 2003;Takara et al, 2002;Takara et al, 2005). Cells were precultured for 24 hr as mentioned above, and then the medium was exchanged for one containing paclitaxel, doxorubicin, cisplatin, or 5-fluorouracil at various concentrations with or without the ABM extract (0.1, 0.5, and 1 µg/ml).…”

Section: Wst-1 Colorimetric Assaymentioning

confidence: 99%

Effects ofAgaricus BlazeiMurill Extract on Sensitivity to Chemotherapeutic Agents in HeLa Cells and its Resistant Sublines

Takara

Shin

Obata

et al. 2008

Journal of Dietary Supplements

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Agaricus blazei Murill (ABM; Japanese name: Kawahiratake or Agarikusutake) extract is a widely used dietary supplement. However, limited information is available on the effects of the extract on the effectiveness of the chemotherapeutic agents. In this study, we examined the effects of ABM extract (Kyowa Wellness Co., Ltd.) on sensitivity to chemotherapeutic agents, paclitaxel and doxorubicin as MDR1/P-glycoprotein substrates, and cisplatin and 5-fluorouracil as non-substrates, in human cervical carcinoma HeLa cells, and paclitaxel-resistant and cisplatin-resistant derivatives (HeLa/TXL and HeLa/CDDP, respectively). The extract had no growth inhibitory effects on HeLa and the resistant cells at concentrations ranging from 7.6 × 10(-4) μ g/ml to 8.0 × 10(2)μ g/ml, indicating no remarkable cytotoxic activity in vitro. In the presence of 0.1, 0.5, and 1 μ g/ml of ABM extract, sensitivity to paclitaxel, cisplatin and 5-fluorouracil did not change in HeLa, HeLa/TXL and HeLa/CDDP cells. However, the extract reduced sensitivity to doxorubicin in HeLa/TXL and HeLa/CDDP cells in a concentration-dependent manner. In conclusion, the concomitant use of ABM extract minimally affected sensitivity to various chemotherapeutic agents in HeLa cells and resistant sublines in vitro.

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Effects of Reactive Oxygen Species on Cell Proliferation and Death in HeLa Cells and Its MDR1-Overexpressing Derivative Cell Line.

Cited by 21 publications

References 15 publications

Long‐Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF‐7 Cells

Long‐Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF‐7 Cells

Optimal Conditions for Labelling of 3T3 Fibroblasts with Magnetoliposomes without Affecting Cellular Viability

Effects ofAgaricus BlazeiMurill Extract on Sensitivity to Chemotherapeutic Agents in HeLa Cells and its Resistant Sublines

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