Biological trickling filters are widely used for sewage treatment. This study models a biological trickling filter based on an anaerobic–anoxic–oxic process (A2O–BTF), established by a combination of aerobic and anaerobic technology. The performance and operational parameters were analyzed using Sumo, a commercially available wastewater treatment process (WWTP) simulation software. The wastewater treatment performance of the anaerobic–anoxic–oxic process biological trickling filter (A2O–BTF), the conventional three-stage biological trickling filter (Three-Stage–BTF), and the single-stage biological trickling filter (Single–BTF) was compared, which indicated the higher performance of A2O–BTF in terms of COD, TN, NH3-N, and TP removal. The operational parameters of A2O–BTF were optimized by Sumo simulation software, and the results showed that the removal efficiency of pollutants was increased by raising the temperature to the range of 13.94–21.60 °C. The dissolved oxygen (DO) in the aerobic reactor enhanced removal efficiency under a saturation concentration of 2.2–2.6 g O2/m3. In addition, the optimization of the reflux ratio promoted the removal efficiency of the pollutants, indicated by the maximum removal efficiency of COD and TN, achieved at the reflux ratio of 2.25, and that of NH3-N and TP, achieved at a reflux ratio of 0.75. This study provides a proof-in-concept demonstration that software modeling can be a useful tool for assisting the optimization of the design and operation of sewage treatment processes.