Effects of recombinant gonadotropin hormones on the gonadal maturation in the spotted scat, Scatophagus argus

Zhang, Guang; Wu, Wei; Su, Maoling; Zhang, Junbin

doi:10.1016/j.aquaculture.2017.10.017

Cited by 14 publications

(5 citation statements)

References 46 publications

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“…In O. niloticus , star mRNA levels in testes were greatly enhanced by injection of human chorionic gonadotropin (hCG) [ 46 ]. Moreover, recombinant follicle stimulating hormone (FSH)/luteinizing hormone (LH) administration increased the star and hsd11b1 expressions, E 2 /11-KT levels, and finally promoted the ovary and testicular development in S. argus [ 47 ]. Thus, understanding the expression and endocrine regulation of steroidogenic genes would greatly help us establish effective methods for controlling reproduction in D. hystrix aquaculture, such as multiple gonadotropin-releasing hormone agonist (GnRHa) injections or implants that are commonly utilized for the artificial induction of oocyte maturation/ovulation and spermination in fish.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Gonadal Transcriptomes Uncovers Reproduction-Related Genes with Sexually Dimorphic Expression Patterns in Diodon hystrix

Chen

Wang

et al. 2021

Animals

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Diodon hystrix is a new and emerging aquaculture species in south China. However, due to the lack of understanding of reproductive regulation, the management of breeding and reproduction under captivity remains a barrier for the commercial aquaculture of D. hystrix. More genetic information is needed to identify genes critical for gonadal development. Here, the first gonadal transcriptomes of D. hystrix were analyzed and 151.89 million clean reads were generated. All reads were assembled into 57,077 unigenes, and 24,574 could be annotated. By comparing the gonad transcriptomes, 11,487 differentially expressed genes were obtained, of which 4599 were upregulated and 6888 were downregulated in the ovaries. Using enrichment analyses, many functional pathways were found to be associated with reproduction regulation. A set of sex-biased genes putatively involved in gonad development and gametogenesis were identified and their sexually dimorphic expression patterns were characterized. The detailed transcriptomic data provide a useful resource for further research on D. hystrix reproductive manipulation.

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Section: Discussionmentioning

confidence: 99%

Comparison of Gonadal Transcriptomes Uncovers Reproduction-Related Genes with Sexually Dimorphic Expression Patterns in Diodon hystrix

Chen

Wang

et al. 2021

Animals

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“…The upregulation of stAR2 by tilPE observed in our experiment concurs with a recent study in another fish species, the Spotted scat. There, recombinant gonadotropins were injected once into males and females and a rise in stAR2 expression could be measured after 14 days (68). We assume that the induced rise of stAR2 gene expression in Nile tilapia testis culture by tilPE, hCG and the mixture of both is due to direct effects of the gonadotropic hormones, because we could detect transcripts of both fshr and lhcgr in freshly dissected tilapia testis and also after 7 days of culture (data not shown).…”

Section: Gene Expression Of the Leydig Cell Markers Star2 And Cyp11b2mentioning

confidence: 96%

An ex vivo Approach to Study Hormonal Control of Spermatogenesis in the Teleost Oreochromis niloticus

et al. 2020

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As the male reproductive organ, the main task of the testis is the production of fertile, haploid spermatozoa. This process, named spermatogenesis, starts with spermatogonial stem cells, which undergo a species-specific number of mitotic divisions until starting meiosis and further morphological maturation. The pituitary gonadotropins, luteinizing hormone, and follicle stimulating hormone, are indispensable for vertebrate spermatogenesis, but we are still far from fully understanding the complex regulatory networks involved in this process. Therefore, we developed an ex vivo testis cultivation system which allows evaluating the occurring changes in histology and gene expression. The experimental circulatory flow-through setup described in this work provides the possibility to study the function of the male tilapia gonads on a cellular and transcriptional level for at least 7 days. After 1 week of culture, tilapia testis slices kept their structure and all stages of spermatogenesis could be detected histologically. Without pituitary extract (tilPE) however, fibrotic structures appeared, whereas addition of tilPE preserved spermatogenic cysts and somatic interstitium completely. We could show that tilPE has a stimulatory effect on spermatogonia proliferation in our culture system. In the presence of tilPE or hCG, the gene expression of steroidogenesis related genes (cyp11b2 and stAR2) were notably increased. Other testicular genes like piwil1, amh, or dmrt1 were not expressed differentially in the presence or absence of gonadotropins or gonadotropin containing tilPE. We established a suitable system for studying tilapia spermatogenesis ex vivo with promise for future applications.

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“…It is also a popular aquaria fish due to its colorful appearance, strong adaptability, and easiness to be cultured indoors (Gupta, 2016). For these reasons, studies focusing on its reproduction biology are on the rise, including reproduction endocrinology (Jiang et al, 2017;Zhang et al, 2018;Mandal et al, 2020), sex determination and differentiation (Mustapha et al, 2018(Mustapha et al, , 2021He et al, 2019), and artificial breeding (Cai et al, 2010;Gandhi et al, 2014;Mandal et al, 2021). Its artificial breeding is successful in several countries, including China (Cai et al, 2010) and India (Mandal et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Liver Transcriptomic Analysis of the Effects of Dietary Fish Oil Revealed a Regulated Expression Pattern of Genes in Adult Female Spotted Scat (Scatophagus argus)

et al. 2021

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Despite the significance of n-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA) in fish oil in promoting the maturation of female broodstocks, the detailed mechanism of the effect on the expression of hepatic reproduction-related genes is still unclear. In this study, transcriptome sequencing was used to analyze the effect of the higher dietary n-3 LC-PUFA level on gene expression in the liver of adult females spotted scat. Two-year-old female spotted scat (average weight, 242.83 ± 50.90 g) were fed with diets containing 8% fish oil (FO) or 8% soybean oil (SO) for 40 days. The fatty acid profile in the serum, liver, and ovary was analyzed, and high proportions of n-3 LC-PUFA were observed in the FO group. The final average fish body weight and gonadosomatic index were similar between the FO and SO groups. The serum vitellogenin (Vtg) and hepatosomatic index (HSI) of the FO group were significantly higher and lower than that of the SO group, respectively. Comparatively, the liver transcriptome analysis showed 497 upregulated and 267 downregulated genes in the FO group. Among them, the expression levels of three estrogen-regulated genes (i.e., Vtga, Vtgb, and Zp4) were significantly higher in the FO than in the SO group. This expression pattern could be related to the upregulation of Hsd17b7 (the key gene for the synthesis of liver steroid hormone) and the downregulation of the Hsp90 (the estrogen receptor chaperone). The expression levels of Foxo1a and Lep, which are involved in the lipid metabolism, decreased significantly in the FO group, which may be related to the lower level of HSI in the FO group. The genes related to liver LC-PUFA absorption and transport, Fabp2 and Mfsd2ab, were significantly upregulated in the FO group, indicating that fish actively adapt to high-fish-oil diets. In brief, high-fish-oil diets can influence the expression of genes related to liver n-3 LC-PUFA metabolism and reproduction, inhibit the accumulation of liver fat, and promote the liver health and gonad development. This study will contribute to clarifying the mechanism of dietary n-3 LC-PUFA on promoting reproductive development in teleost fish.

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Effects of recombinant gonadotropin hormones on the gonadal maturation in the spotted scat, Scatophagus argus

Cited by 14 publications

References 46 publications

Comparison of Gonadal Transcriptomes Uncovers Reproduction-Related Genes with Sexually Dimorphic Expression Patterns in Diodon hystrix

Comparison of Gonadal Transcriptomes Uncovers Reproduction-Related Genes with Sexually Dimorphic Expression Patterns in Diodon hystrix

An ex vivo Approach to Study Hormonal Control of Spermatogenesis in the Teleost Oreochromis niloticus

Liver Transcriptomic Analysis of the Effects of Dietary Fish Oil Revealed a Regulated Expression Pattern of Genes in Adult Female Spotted Scat (Scatophagus argus)

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