Effects of recombinant retroviral vector mediated human insulin like growth factor-1 gene transfection on skeletal muscle growth in rat

Rong, Shuling; Yongxin, Lu; Liao, Yuhua; Wang, Xiaolin; Guo, Hao; Chang, Chao; Gao, Yu; Mi, Shao-Hua; Jian-ping, Wan

doi:10.1097/00029330-200612010-00009

Cited by 3 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Primary-myoblasts and hIGF-1-myoblasts were prepared according to previously used method in our laboratory [5] .…”

Section: Methodsmentioning

confidence: 99%

“…The use of retroviral vectors in our past studies resulted in the stable integration of the hIGF-1 gene into the myoblast genome and long-term expression for 6 weeks after they were implanted in vivo. Moreover, it promotes the growth of skeletal muscle myoblasts without bringing about biological changes in transduced cells [5] . Therefore, we hypothesized that IGF-1 might promote the growth of cardiomyocytes in the same way [5] .…”

mentioning

confidence: 99%

“…Moreover, it promotes the growth of skeletal muscle myoblasts without bringing about biological changes in transduced cells [5] . Therefore, we hypothesized that IGF-1 might promote the growth of cardiomyocytes in the same way [5] . To test our hypothesis, in this study, we injected the hIGF-1-myoblasts into post-infraction rats and detected the expression of p16 INK4a (INK4a, an inhibitor of CDK4) and the proliferating cell nuclear antigen (PCNA) protein into myocardium to see if hIGF-1-myoblast therapy can provide a new alternative for the clinical treatment of heart failure.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Change of p16INK4a and PNCA protein expression in myocardium after injection of hIGF-1 gene modified skeletal myoblasts into post-infarction rats

Gao

Yongxin

et al. 2008

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

View full text Add to dashboard Cite

This study examined the change of p16(INK4a) and PCNA protein expression in myocardium after injection of hIGF-1 gene modified skeletal myoblasts into post-infarction rats. HIGF-1 gene modified skeletal myoblasts (hIGF-1-myoblasts) were injected into hind limb muscles of 18 post-infraction rats (experimental group). Primary-myoblasts were injected into 18 post-infraction rats (control group) and 12 non-infarction rats (sham group). Expression of p16(INK4a) and PCNA protein in myocardiums were separately detected immunocytochemically 1, 2 and 4 weeks after the injection. The level of hIGF-1 and rIGF-1 protein in serum and myocardium were detected by enzyme-linked immunosorbent assay (ELISA). Compared with the sham group, the percentage of p16(INK4a) and PCNA positive cells reached a peak after 1 week in the control group and the experimental group (P<0.01). Moreover, the percentage of p16(INK4a)-positive cells in the experimental group was lower than in control group whereas the percentage of PCNA-positive cells was lower in the control group than in the experimental group (P<0.01). The percentage of p16(INK4a)-positive cells in the experimental group and the percentage of PCNA-positive cells in the control group were close to that in the sham group from the 2nd week (P>0.05). ELISA analysis disclosed that the myocardium level of rIGF-1 protein increased gradually in the controls and especially in the experimental group (P<0.01). The serum level of rIGF-1 decreased significantly in post-infraction rats, but these conditions were improved in the experimental group (P<0.01). The hIGF-1 protein in serum and myocardium were detected from the 1st week to the 4th week in the experimental group. Statistical analysis revealed significant associations of myocardium level of hIGF-1 protein with expression of p16(INK4a) and PCNA protein (r=-0.323, P<0.05; r=0.647, P<0.01). It is concluded that genetically hIGF-1-myoblast provides a means for constant synthesis and release of hIGF-1. It could not only improve the expression of rIGF-1 and PCNA protein in myocardium, but also suppress the expression of p16(INK4a) protein for 30 days in post-infraction rats. Myoblasts-mediated IGF-1 gene therapy may provide a new alternative for the clinical treatment of heart failure.

show abstract

“…Primary-myoblasts and hIGF-1-myoblasts were prepared according to previously used method in our laboratory [5] .…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation