Effects of recovery from immobilization stress on striatal preprodynorphin- and kappa opioid receptor-mRNA levels of the male rat

Lucas, Louis; Dragisic, Tina; Duwaerts, Caroline C.; Swiatkowski, Michael; Suzuki, Hideo

doi:10.1016/j.physbeh.2011.06.017

Cited by 15 publications

(11 citation statements)

References 67 publications

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“…In contrast to our CORT findings, however, a number of previous studies have demonstrated that stress increases CORT levels (Blanchard, Sakai, McEwen, Weiss, & Blanchard, 1993;Blanchard et al, 1995;Lucas et al, 2004;Lucas et al, 2011;Lucas et al, 2007;Rabasa et al, 2011;Tamashiro et al, 2004). One possible reason explaining our CORT findings may be that nonimmobilized rats became as stressed as immobilized rats because they were housed individually through immobilization sessions and behavioral assessments.…”

Section: Hormonal Outcomescontrasting

confidence: 99%

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Aggression is suppressed by acute stress but induced by chronic stress: Immobilization effects on aggression, hormones, and cortical 5-HT1B/ striatal dopamine D2 receptor density

Yohe

Suzuki²,

Lucas

2012

Cogn Affect Behav Neurosci

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Although it has been established by a number of investigators that a variety of stressors are associated with the induction of aggressive behavior, two specific issues remain unanswered. First, it is unclear whether the contexts surrounding stressors (e.g., stressor length and chance of winning over opponents) change outcomes regarding aggressive behavior. Second, if a relationship exists between stress and aggressive behavior, altered levels of stressrelated hormone (e.g., corticosterone [CORT]), as well as aggression-related biomarkers (e.g., testosterone [T], density of prefronto-cortical 5-HT 1B receptor and striatal dopamine D 2 receptor [D2r]) may contribute to changes in aggressive behavior. Thus, we examined how immobilization (with a 1-, 5-, or 10-day exposure) would impact (1) a longitudinal course of aggression toward different-sized opponents, (2) levels of CORT and T, and (3) densities of 5-HT 1B receptor (5-HT1Br) in the prefrontal cortex (PFC) and D2r in the striatum. It was found that, regardless of small or large opponents, a single 2-h exposure to immobilization reduced aggressive behavior (stress-suppressed aggression) over time, whereas repeated (10-day) exposure to immobilization escalated aggressive behavior (stress-induced aggression). These stress effects persisted up to 1 week of recovery from immobilization stress. Moreover, immobilized rats demonstrated elevated levels of T, but not CORT, as compared with controls. Finally, acute immobilization altered D2r densities in the shell of the nucleus accumbens, and chronic immobilization changed 5-HT1Br in the PFC, including the downregulation of 5-HT1Br densities in the right prelimbic and orbitolateral cortices. The potential relationships among stress, aggression, and 5-HT1Br/D2r roles are discussed.

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Section: Hormonal Outcomescontrasting

confidence: 99%

“…Consistent with a previous finding (Lucas, Dragisic, Duwaerts, Swiatkowski, & Suzuki, 2011), acute exposure to immobilization did not induce a change in weight. Interestingly, we did not find a decrease in weight gain even after chronic immobilization or even when a recovery period was taken into account.…”

Section: Immobilization and Body Weightsupporting

confidence: 92%

Aggression is suppressed by acute stress but induced by chronic stress: Immobilization effects on aggression, hormones, and cortical 5-HT1B/ striatal dopamine D2 receptor density

Yohe

Suzuki²,

Lucas

2012

Cogn Affect Behav Neurosci

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“…The literature evidences the association between dynorphin and dysphoria; therefore, the role of dynorphin has also been investigated in the development of depression. The repeated exposure of immobilization stress decreases the motivational behavior in animals and correlate with changes in the dynorphin/-opioid receptor system in the different brain regions (Lucas et al, 2011). Shirayama and coworkers documented the importance of both dynorphin A and B in stress-induced depression and reported that during "learned helplessness", the levels of dynorphin A and B are increased in the hippocampus and nucleus accumbens regions.…”

Section: Effect Of Dynorphin System On Stress-related Anxiety and Depmentioning

confidence: 99%

Stress and opioids: Role of opioids in modulating stress-related behavior and effect of stress on morphine conditioned place preference

Bali

Randhawa

Jaggi

2015

Neuroscience & Biobehavioral Reviews

101

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“…The rich reservoir offered by the different RNA isoforms could be relevant to various needs for receptor production and/or distribution under different physiological states. The complexity of the KOR gene regulatory elements might have contributed to the controversy previously found in the field [i.e., stress-induced KOR mRNA reduction (14) vs. increase (15) in the striatum, no effect (16) vs. increased (15) KOR mRNA in the nucleus accumbens]. To this end, it is important to note that the different isoforms of KOR mRNA exhibit distinct stability, translation efficiency, RNA transport efficiency, and CNS expression patterns (23,37).…”

Section: Discussionmentioning

confidence: 99%

“…Exposure to stress and recovery affects dynorphin and KOR mRNA levels in hypothalamus, hippocampus, striatum, and amygdala (14)(15)(16)(17)(18), and differential KOR mRNA levels were detected in stresssensitive rodent strains [Wistar-Kyoto rats (19), BALB/cJ, and DBA/2J mice (20)]. However, these results are complicated by inconsistent findings.…”

mentioning

confidence: 99%

Stress-induced epigenetic regulation of κ-opioid receptor gene involves transcription factor c-Myc

Flaisher-Grinberg

Persaud

Loh

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Exposure to stress is associated with adverse emotional and behavioral responses. Whereas the κ-opioid receptor (KOR) system is known to mediate some of the effects, it is unclear whether and how stress affects epigenetic regulation of this gene. Because the KOR gene can use two promoters (Pr1 and Pr2) and two polyadenylation signals (PA1 and PA2), it is also interesting whether and how these distinct regulatory mechanisms are differentially modulated by stress. The current study examined the effects of stress on these different regulatory mechanisms of the KOR gene. Results showed that stress selectively increased the expression of KOR mRNA isoforms controlled by Pr1 and terminated at PA1 in specific brain areas including the medial-prefrontal cortex, hippocampus, brainstem, and sensorimotor cortex, but not in the amygdala or hypothalamus. These effects correlated with altered epigenetic state of KOR Pr1 chromatin, as well as elevation and increased recruitment of the principal transcription factor c-Myc, which could activate Pr1. Stress-induced modulation of Pr1 was further validated using glutamate-sensitive murine hippocampal cell line, HT22. The results revealed a common molecular mechanism underlying the effect of stress on selected chromatin regions of this gene at the cellular level and in the context of whole animal and identified a critical role for c-Myc in stress-triggered epigenetic regulation of the KOR gene locus. This study sheds light on the mechanisms of stress-induced epigenetic regulation that targets specific chromatin segments and suggests certain KOR transcripts and its principal transcription factor c-Myc as potential targets for brain-area-specific intervention.

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Effects of recovery from immobilization stress on striatal preprodynorphin- and kappa opioid receptor-mRNA levels of the male rat

Cited by 15 publications

References 67 publications

Aggression is suppressed by acute stress but induced by chronic stress: Immobilization effects on aggression, hormones, and cortical 5-HT1B/ striatal dopamine D2 receptor density

Aggression is suppressed by acute stress but induced by chronic stress: Immobilization effects on aggression, hormones, and cortical 5-HT1B/ striatal dopamine D2 receptor density

Stress and opioids: Role of opioids in modulating stress-related behavior and effect of stress on morphine conditioned place preference

Stress-induced epigenetic regulation of κ-opioid receptor gene involves transcription factor c-Myc

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