Effects of reduced humidity and antitranspirants in acclimatizing micropropagated Citrus plantlets.

Hazarika, B. N.; Parthasarathy, V. A.

doi:10.37855/jah.2002.v04i01.09

Cited by 6 publications

(2 citation statements)

References 6 publications

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“…The rooting of elongated shoots on MS medium supplemented with NAA or IBA has been well documented in Stevia rebaudiana (Verma et al 2011), Simmondsia chinensis (Kumar et al 2013) and Vitex negundo (Groach et al 2014). In the culture laboratory, in vitro raised plants are constantly maintained under a controlled environment of high humidity, low light intensity, photoperiod, optimum temperature, supplementary sugar supply and growth regulators (Hazarika, Bora 2006;Yadav et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Indirect organogenesis in Ephedra foliata

Singh

Yadav²,

Singh

2022

EEB

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Ephedra foliata Boiss. ex C.A. Mey (Ephedraceae) is a well-known source of the alkaloid ephedrine used for the treatment of chronic asthma and associated respiratory ailments. This investigation was conducted to standardize an efficient regeneration protocol for E. foliata via indirect organogenesis through intermodal explants. Maximum callus induction (80.3%) was achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg L‑1 2,4‑dichlorophenoxyacetic acid + 0.5 mg L‑1 kinetin and additives (50 mg L‑1 ascorbic acid and 25 mg L‑1 citric acid). The maximum shoot regeneration (n = 5.27) was achieved on MS medium containing 6.0 mg L‑1 kinetin, followed by 5.0 mg L‑1 6‑benzylamino purine (n = 4.27). MS half strength medium with 3.0 mg L‑1 α‑naphthalene acetic acid resulted in the highest rooting percentage (32%). Sixty percent of the plantlets survived during acclimatization and were successfully transferred under field conditions. These plants and callus will be a suitable source of plant material for mass multiplication, genetic modification for enhanced bioactive constituents, and germplasm conservation.

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Section: Discussionmentioning

confidence: 99%

Indirect organogenesis in Ephedra foliata

Singh

Yadav²,

Singh

2022

EEB

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“…Application of anti-transpirants can propel the hardening process and open field establishment of the in vitro raised plants in citrus (Hazarika and Parthasarathy, 2002). The reduced water loss and maximum ex vitro survival of citrus with in vitro application of antitranspirant 8-hydroxyquinoline (2 mg/L).…”

Section: Citrusmentioning

confidence: 99%

Ex-vitro Establishment of Tissue Cultured plants in Fruit Crops-A Review

Mahendra¹,

Chauhan²,

Sharma³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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Challenges and insights in the acclimatization step of micropropagated woody plants

Grzelak,

Pacholczak,

Nowakowska

2024

Plant Cell Tiss Organ Cult

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Acclimatization is the last step of plants in vitro propagation while it comes to alter the environment conditions from tailor for plant requirements, sterile laboratory to external ex vitro conditions. Consequently, during acclimatization it may reach to plants mortality due to abiotic and biotic stresses occurring in external environment (high temperature, light intensity, low air relative humidity and the presence of microorganisms and pathogens in soil). Micropropagated plants develop alter organs on morphological, anatomical or biochemical levels which must be correct during acclimatization to improve adaptation of plants to ex vitro conditions. Therefore, there is a need to create acclimatization protocols to improve the effectiveness of this vegetative propagation method. So far there were formulated facilities as pre-acclimation, in vitro acclimatization, water cultures, CO2 enrichment, application of biostimulators and plant growth regulators. In following research we focused on describing previous achievements about the changes occurring in woody plants by micropropagation and summary of achievements in acclimatization of micropropagated woody plants. This article summary the current state of knowledge on acclimatization for various range of woody species by anatomical, physiological and biochemical levels. There are described ways to increase the survival of acclimatized plants and minimize acclimatization stress it also collects substances used so far to enhance plant growth during acclimatization.

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Effects of reduced humidity and antitranspirants in acclimatizing micropropagated Citrus plantlets.

Cited by 6 publications

References 6 publications

Indirect organogenesis in Ephedra foliata

Indirect organogenesis in Ephedra foliata

Ex-vitro Establishment of Tissue Cultured plants in Fruit Crops-A Review

Challenges and insights in the acclimatization step of micropropagated woody plants

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