Based on the nutrient requirement of guppy, Poecilia reticulata fingerlings as reported earlier, nine experimental diets with 300 g protein, 100 g lipid and 16.72 MJ digestible energy/kg diet were formulated using snail meat (D-1), freshwater fish processing waste (D-2), surimi by-product (D-3), chicken offal (D-4), earthworm (D-5), squid (D-6), mussel (T-7), chicken liver (T-8) and lean prawn (T-9) as major protein source in addition to fish meal and peanut oil cake and fed ad libitum to the fish (0.27 ± 0.01 g) for a period of 60 days. Twenty-seven indoor circular fiber-reinforced plastic tanks (10 fish/tank) with 40 L of water were used for rearing the fish. At the end of the experiment, it was found that the guppy fed surumi by-product, squid, mussel and lean prawn meal diets had significantly higher (p \ 0.05) weight gain, specific growth rate and protein efficiency ratio and lowest (p \ 0.05) food conversion ratio than the snail, freshwater fish processing waste, chicken offal, earthworm and chicken liver meal diets and therefore, these four could be used as dietary protein source in formulating the diets for guppy. However, to formulate the cost-effective diets for guppy fingerlings, the use surimi by-product is suggested as it is being discarded as waste material and therefore, available free of cost.