Effects of replacing fish meal with mussel (Cristaria plicata) meat on growth, digestive ability, antioxidant capacity and hepatic IGF-I gene expression in juvenile Ussuri catfish (Pseudobagrus ussuriensis)

Abstract

An 8-week feeding trial was implemented to evaluate the effects of replacing fish meal (FM) with mussel (Cristaria plicata) meat (MM) on growth, digestive ability, antioxidant capacity and hepatic insulin-like growth factor I (IGF-I) gene expression of juvenile Ussuri catfish (Pseudobagrus ussuriensis). Three isonitrogenous and isolipidic diets were formulated to include 0, 177.5 and 355.1 g/kg of MM, accordingly, replacing 0% (M 0 , control), 50% (M 1 ) and 100% (M 2 ) of FM protein, respectively. The results showed that the final body weight, weight gain, specific growth rate and feed intake were gradually decreased with dietary MM protein levels increased, but there were no significant difference between M 0 and M 1 groups (p > 0.05). The protein efficiency ratio was increased significantly with dietary MM inclusion (p < 0.05). The apparent digestibility coefficient of dry matter, crude lipid and gross energy gradually increased with increasing dietary MM protein levels, but the apparent digestibility coefficient of crude protein was not significantly affected by MM protein supplementation (p > 0.05). Fish fed diet, M 0 and M 1 remained unaffected significantly on activities of alpha-amylase and pepsin (p > 0.05), but fish fed diet M 2 had the highest activities of alpha-amylase and pepsin. Fish fed diet M 1 or M 2 had significantly lower hepatic total antioxidant capacity, superoxide dismutase and the higher malondialdehyde level compared to fish fed diet M 0. In addition, no significant difference was observed in hepatic IGF-I gene expression level for fish fed diet M 0 and diet M 1 , and fish fed diet M 2 showed significantly lower hepatic IGF-I gene expression level. Therefore, we can conclude that MM protein can successfully substitute 50% of FM protein without significantly negative effect on growth, nutrient utilization, and hepatic IGF-I gene expression for juvenile Ussuri catfish, but the antioxidant capacity was negatively affected in the present study, what is more, the total replacement of FM by MM in diet may result in the inhibition of the growth and antioxidant capacity of fish. K E Y W O R D Santioxidant capacity, digestive ability, growth, mussel meat, ussuri catfish The first two authors contributed equally to this work.

Early-Life Nutrition Interventions Improved Growth Performance and Intestinal Health via the Gut Microbiota in Piglets

“…qRT-PCR reaction was programmed as follows: 94°C for 30 s, and followed by 40 cycles for 5 s at 94°C, 30 s at 60°C and 10 s at 72°C. Relative expression levels of all genes were determined using the 2 −ΔΔCT method ( 17 ).…”

“…Expression levels of the target genes were normalized to the housekeeping genes β-actin and GAPDH as endogenous controls. qRT-PCR reaction was programmed as follows: 94 • C for 30 s, and followed by 40 cycles for 5 s at 94 • C, 30 s at 60 • C and 10 s at 72 • C. Relative expression levels of all genes were determined using the 2 − CT method (17).…”

Yeast culture improve CCl 4 ‐induced liver damage, inflammatory response via inhibition of TLR2/NF‐kB signaling pathway expression in Pseudobagrus ussuriensis

“…However, this fish is facing the problem of immune suppression and liver damage with the development of intensive farming. To date, some experiments about dietary nutrition requirement of this fish have been conducted and the effect of different vegetable protein (soybean meal, vegetable meal, cotton meal and corn protein) and animal protein (meat and bone meal, mussel meal) replacing fish meal had been studied in our laboratory (Bu et al, 2017;Luo, 2019;Wang et al, 2020). Moreover, a preliminary study about the health function of YC on Pseudobagrus ussuriensis has been explored (Bu et al, 2019).…”

An Evaluation of Yeast Culture Supplementation in the Diet of Pseudobagrus ussuriensis: Growth, Antioxidant Activity, Nonspecific Immunity, and Disease Resistance to Aeromonas hydrophila

“…However, due to the large-scale and intensive fish farming, the shortage of fishmeal is prominent. To date, several studies have reported feasibility in replacing fishmeal with different proteins, including corn gluten meal [15], rapeseed meal [16], cottonseed meal [17], meatand bone meal [18] as well as mussel (Cristaria plicata) meat meal [19] in P. ussuriensis. But there is limited message regarding the potential role of dietary YC as partial FM substitute on growth performance and healthy status for P. ussuriensis.…”