Effects of replacing fishmeal with dietary dried distillers grains with solubles on growth, serum biochemical indices, antioxidative functions, and disease resistance for Litopenaeus vannamei juveniles

Gyan, Watson Ray; Yang, Qihui; Dong, Xiaohui; Chi, Shuyan; Liu, Hongyu; Zhang, Shuang

doi:10.1016/j.aqrep.2021.100821

Cited by 17 publications

(17 citation statements)

References 66 publications

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“…SOD GSH-Px are antioxidant enzyme systems that are closely related to free radical scavenging functions and protect important cellular macromolecules and organelles from oxidative damage (Hu et al, 2019). MDA is the end-product of lipid peroxidation triggered by a free radical attack on polyunsaturated fatty acids in biological membranes, and its concentration reflects the degree of cellular damage (Gyan et al, 2021). A study on the effect of GML on the antioxidant capacity for Lateolabrax maculatus (Sun et al, 2021) shows that GML significantly increases SOD activity and GSH-Px concentration and significantly reduces MDA concentration.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

et al. 2022

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Glycerol monolaurate (GML), a representative fatty acid glyceride, was used to promote growth. This study investigated the effects of GML in diets on growth, serum biochemical parameters, antioxidant capacity, liver morphology and lipid metabolism for juvenile pompano Trachinotus ovatus. Six groups of T. ovatus (mean weight = 14.00 ± 0.70 g) were fed with six diets, containing varying GML levels of 0.00% (G0), 0.05% (G5), 0.10% (G10), 0.15% (G15), 0.20% (G20), and 0.25% (G25) for 8 weeks. Fish were reared for in floating cages (length: 1 m; width: 1 m; and height: 2 m). The highest weight gain rate (WGR), specific growth rate (SGR), and protein deposit rate (PDR) were observed in the G15 group (P < 0.05). Hepatic glutathione peroxidase, total antioxidant capacity and superoxide dismutase activities were significantly increased in the G10 and G15 groups compared with the G0 group (P < 0.05), whereas hepatic malondialdehyde (MDA) was significantly reduced in the G10 and G15 groups (P < 0.05). The alkaline phosphatase activity was significantly higher in the G5, G10, G15, and G20 groups than in the G0 group (P < 0.05), and the highest AKP activity was observed in the G15 group. Alanine aminotransferase (ALT) activity was significantly lower in the G10, G15, G20, and G25 groups than in the G0 group (P < 0.05). The addition of GML to the feed significantly reduced triglyceride (TG) content (P < 0.05). In comparison with the G0 group, the G15, G20, and G25 groups had significantly lower total cholesterol content and significantly higher high-density lipoprotein content (P < 0.05). Low-density lipoprotein content was significantly lower in the G5 and G10 groups than in the G0 group (P < 0.05). Serious vacuolation occurred in the G0 group, but the cell boundaries in all added groups were obvious, and the rate of intracellular vacuolization decreased. As the GML level increased, carnitine palmitoyl-transferase Ι mRNA level was significantly up-regulated (P < 0.05). The expression of fatty acid synthesis in the G10, G15, G20, and G25 groups was significantly lower than that in the G0 group (P < 0.05). The expression of sterol regulatory element-binding protein-Ι was significantly lower in the G10, G15, G20, and G25 groups than in the G0 group (P < 0.05). Based on the broken-line model of WGR and PDR corresponding to GML levels, the optimum addition level of dietary GML was 0.14%-0.16% for juvenile T. ovatus. In conclusion, 0.15% GML significantly improved the growth performance, hepatic antioxidant ability, and lipid metabolism and protected the liver for juvenile pompano T. ovatus.

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Section: Discussionmentioning

confidence: 99%

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

et al. 2022

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“…Hemolymph is important for fat absorption and transport, and TG is transported as an energy substance between adipose tissue and the liver via hemolymph (Gyan et al, 2021). The fitness of aquatic animals which can be assessed using blood parameters (Lemaire et al, 1991).…”

Section: Figurementioning

confidence: 99%

Effect of Schizochytrium limacinum supplementation to a low fish-meal diet on growth performance, lipid metabolism, apoptosis, autophagy and intestinal histology of Litopenaeus vannamei

Yao

Lin²,

Shi³

et al. 2022

Front. Mar. Sci.

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In this experiment, we aimed to evaluate the relationship between the addition of Schizochytrium limacinum to low fish meal diets on growth performance, apoptosis, autophagy, lipid metabolism, and intestinal health of Lipenaeus vanamei. The diet containing 25% fish meal was used as a positive control (FM) and the other three diets contained 15% fish meal and were supplemented with 0, 0.3, and 0.6% S. Limacinum (LF, LFLD, LFHD). The shrimp (0.22 ± 0.00 g) were divided into four replicates of 40 shrimp per tank and fed four times daily to apparent satiation for 8 weeks. Results showed that the final weight (FBW) and weight gain rate (WGR) of shrimp fed FM and LFHD diets were significantly increased compared to those fed the LFLD diet (P<0.05), and there was no significant difference in survival rate (SR) and feed conversion rate (FCR) among the groups (P>0.05). Supplementation of S. Limacinum in low fish meal diets had no effects on shrimp body composition (P<0.05). There were significant differences (P<0.05) in low-density lipoprotein (LDL-C) glucose (GLU), triglycerides (TG), and total cholesterol (TC) in the hemolymph of shrimp fed the LF diet compared to those fed the LFLD and LFHD diets. HE staining and transmission electron microscopy (TEM) results showed that the microvilli height, mucosal folds height, mucosal folds width and muscle layer thickness in the intestine of shrimp fed the LF diet were significantly reduced compared to those fed the other three diets (P<0.05). Swelling of the endoplasmic reticulum and irregular mitochondria in the gut of shrimp fed the LF diet was also observed by TEM, and the endoplasmic reticulum and mitochondria of shrimp fed the LFHD diet returned to a healthy state. Hepatopancreas genes expression results were showed that the gene expression of 5′ -AMP-activated protein kinase (ampk), stearoyl-CoA desaturase (scd1), acetyl-CoA carboxylase 1 (acc1), and malonyl-CoA decarboxylase (mcd) of shrimp fed the LF diet was significantly increased compared to those fed the FM diet (P<0.05). The gene expression of sterol regulatory element-binding protein (srbep) and carnitine palmitoyl transferase 1 (cpt-1) of shrimp fed the LFLD diet was significantly increased compared to those fed the LF diet (P<0.05). The gene expression of acc1, mcd and scd1 of shrimp fed the LFHD diet was significantly reduced compared to those fed the LF diet (P<0.05). Results of genes expression associated with apoptosis in the hepatopancreas showed that the gene expression of B lymphocytoma-2 (bcl-2), BCL2 associated X apoptosis regulator (bax) and cysteinyl aspartate specific proteinase 8 (caspase 8) of shrimp fed the LF diet was significantly reduced compared to those fed the FM diet (P<0.05). The gene expression of bcl-2 of shrimp fed the LFHD diet was significantly reduced compared to those fed the LF diet (P<0.05). Genes related to autophagy in the hepatopancreas showed that the expression of autophagy-related protein 12 (atg 12), autophagy-related protein 13 (atg 13) and beclin1 of shrimp fed LF the diet was significantly reduced compared to those fed the FM diet (P<0.05). The gene expression of atg 12 and atg 13 of shrimp fed the LFHD diet was significantly increased compared to those fed the LF diet (P<0.05). In summary, reducing fish meal is detrimental to the growth performance and intestinal health of shrimp, and 0.6% S. Limacinum supplementation can improve the growth performance, promotes hepatopancreas lipid metabolism, reduces apoptosis, promotes autophagy and improve intestinal health of Litopenaeus vannamei.

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“…They could be connected with the development of enteropathy after the ingestion of gluten and, eventually, cause the degeneration of intestinal epithelium with villous atrophy in vertebrates (Ciccocioppo et al, 2001; Dyduch et al, 1993; March, 2003; Marietta & Murray, 2012b; Marietta et al, 2011; Moss et al, 1996; Perez et al, 2021), when mainly the intestinal epithelium is damaged (Bethune et al, 2008; Ciccocioppo et al, 2001; Moss et al, 1996). However, data on invertebrate animals, which may serve as vectors for cereal proteins, concern especially the synthesis of enzymes in their digestive systems under the influence of cereal proteins or the structure of their internal organs (Broadway & Duffey, 1988; Dvoryakova et al, 2022; Elaidy & Zedan, 2011; Elaidy et al, 2010; Goptar et al, 2012; Gyan et al, 2021). However, there is still no discussion of the effects of cereal proteins at various levels of body organization, i.e., from organs/tissues, through the level of cells, cellular organelles, and their membranes, to changes at the molecular level (Gyan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…However, data on invertebrate animals, which may serve as vectors for cereal proteins, concern especially the synthesis of enzymes in their digestive systems under the influence of cereal proteins or the structure of their internal organs (Broadway & Duffey, 1988; Dvoryakova et al, 2022; Elaidy & Zedan, 2011; Elaidy et al, 2010; Goptar et al, 2012; Gyan et al, 2021). However, there is still no discussion of the effects of cereal proteins at various levels of body organization, i.e., from organs/tissues, through the level of cells, cellular organelles, and their membranes, to changes at the molecular level (Gyan et al, 2021). Such changes should be analyzed according to different periods of exposure to gluten additives in food.…”

Section: Introductionmentioning

confidence: 99%

Effect of gluten on the digestive tract and fat body of Telodeinopus aoutii (Diplopoda)

Błaszczyk

Sosinka

Wilczek

et al. 2022

Journal of Morphology

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Adult specimens or larvae of invertebrates used as food for vertebrates are often maintained close to gluten so they might become vectors for cereal proteins.However, the tissues and internal organs can respond differently in animals with different feeding habits. The midgut epithelium might be a first and sufficient barrier preventing uptake and effects of gluten on the whole body, while the fat body is the main organ that accumulates different xenobiotics. Good models for such research are animals that do not feed on gluten-rich products in their natural environment. The project's goal was to investigate alterations in the midgut epithelium and fat body of the herbivorous millipede Telodeinopus aoutii (Diplopoda) and analyze cell death processes activated by gluten. It enabled us to determine whether changes were intensified or reversed by adaptive mechanisms. Adult specimens were divided into control and experimental animals fed with mushrooms supplemented with gluten and analyzed using transmission electron microscopy, flow cytometry, and confocal microscopy. Two organs were isolated for the qualitative and quantitative analysis: the midgut and the fat body. Our study of the herbivorous T. aoutii which does not naturally feed on gluten containing diet showed that continuous and prolonged gluten feeding activates repair processes that inhibit the processes of cell death (apoptosis and necrosis) and induce an increase in cell viability.

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Effects of replacing fishmeal with dietary dried distillers grains with solubles on growth, serum biochemical indices, antioxidative functions, and disease resistance for Litopenaeus vannamei juveniles

Cited by 17 publications

References 66 publications

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

Effect of Schizochytrium limacinum supplementation to a low fish-meal diet on growth performance, lipid metabolism, apoptosis, autophagy and intestinal histology of Litopenaeus vannamei

Effect of gluten on the digestive tract and fat body of Telodeinopus aoutii (Diplopoda)

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