Effects of resalination on intestinal glucose transport in chickens adapted to low Na+ intakes

Garriga, Carles; Moretó, Miquel; Planas, Joana M.

doi:10.1017/s095806700002039x

Cited by 7 publications

(13 citation statements)

References 11 publications

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“…Sodium-glucose cotransporter 1 is the key factor affecting glucose absorption by the intestine (Kellett 2001;Garriga et al 2000;Wood and Frayhum 2003) and is expressed mainly in the duodenum (Garriga et al 2000). In the present study, LPS injection resulted in upregulated expression of the SGLT1 gene.…”

Section: Discussionsupporting

confidence: 51%

Effects of an early lipopolysaccharide challenge on growth and small intestinal structure and function of broiler chickens

et al. 2011

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Hu, X. F., Guo, Y. M., Li, J. H., Yan, G. L., Bun, S. and Huang, B. Y. 2011. Effects of an early lipopolysaccharide challenge on growth and small intestinal structure and function of broiler chickens. Can. J. Anim. Sci. 91: 379–384. Two experiments were conducted to determine the effect of early exposure to lipopolysaccharide (LPS) on small intestinal structure and function of broiler chickens. Seven-day-old birds were randomly allotted to two equal treatments: an LPS-injected treatment in which the birds were injected intraperitoneally with LPS 500 µg kg−1 body weight (dissolved in 1 mL saline) on 8, 10, 12, 15, 17, and 19 d of age, i.e., on days 1, 3, and 5 d for 2 continuous weeks, and a control treatment (CTRL) in which the birds were similarly injected with 1 mL saline as a placebo. In exp. 1, food intake and weight gain were monitored over the 2 wk, the weight of the small bowel was determined at 14 and 21 d of age and duodenal and jejunal villus height and crypt depth, D-xylose uptake were also measured at 21 d. In exp. 2, additional measurements of the intestinal peristalsis ratio and the BrdU-labeling index and duodenal sodium-glucose co-transporter-1 (SGLT1) mRNA level were made at 21 d of age. The results showed that LPS challenge decreased feed intake, daily gain, duodenal and jejunal villus height and crypt depth, plasma D-xylose concentration and intestinal BrdUrd-labeling index, respectively (P<0.05) as well as small bowel weight at 14 and 21 d of age (P<0.05). Conversely, LPS injection increased SGLT1 mRNA level in the small intestine (P<0.05) and the small intestinal relative weight at 14 (P<0.05) and 21 d of age (P=0.063). Following LPS injection there were non-significant changes in feed conversion ratio and intestinal peristalsis ratio (P>0.05). In conclusion, early LPS challenge delayed the growth of intestine and impaired small intestinal structure and absorptive function.

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Section: Discussionsupporting

confidence: 51%

Effects of an early lipopolysaccharide challenge on growth and small intestinal structure and function of broiler chickens

et al. 2011

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“…The SGLT1 mediates the Na + -dependent uptake of glucose and galactose across the brush border membrance (Garriga et al, 2000) and is considered to be the primary mediator of glucose assimilation in the small intestine (Hediger and Rhoads, 1994). The PepT1 is responsible for transporting di-and tripeptides into epithelial cells (Leibach and Ganapathy, 1996).…”

Section: Discussionmentioning

confidence: 99%

Xylanase supplementation of a wheat-based diet improved nutrient digestion and mRNA expression of intestinal nutrient transporters in broiler chickens infected with Clostridium perfringens

et al. 2014

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Necrotic enteritis caused by Clostridium perfringens has become prevalent in the European Union due to the withdrawal of antibiotics in poultry feed. In an experiment with a 2 × 2 factorial arrangement, 336 one-day-old male broiler chicks (Ross 308) were assigned to 4 groups with or without C. perfringens challenge and fed wheat-based diets supplemented with or without xylanase at 5,500 U/kg of diet. The study aimed to investigate effects of xylanase addition on growth performance as well as nutrient digestion and absorption of C. perfringens-infected broilers. Before challenge (d 0-14), xylanase-supplemented birds had greater ADG and lower feed conversion ratio (FCR; P < 0.05). During infection (d 14-21), challenge tended to decrease ADG (P = 0.063) and significantly increased FCR (P < 0.05), whereas xylanase addition greatly reduced FCR (P < 0.05). Clostridium perfringens infection decreased AME values and apparent ileal digestibility of DM of diets (P < 0.05). Xylanase supplementation increased AME values regardless of infection and apparent ileal digestibility of CP in challenged birds (P < 0.05). Activities of duodenal α-amylase and chymotrypsin and pancreatic trypsin were decreased by C. perfringens infection (P < 0.05). Xylanase supplementation elevated pancreatic chymotrypsin activity and reduced duodenal α-amylase and trypsin activities (P < 0.05). It also decreased jejunal α-amylase activity and increased pancreatic α-amylase as well as jejunal sucrase activities in uninfected birds (P < 0.05). The duodenal mRNA expression of sodium glucose cotransporter 1 (SGLT1), H(+)-dependent peptide transporter 1 (PepT1), and liver fatty acid-binding protein (L-FABP) were downregulated (P < 0.05), but ileal SGLT1 gene expression was increased by infection (P < 0.05). Xylanase addition upregulated expression of jejunal SGLT1, PepT1, and L-FABP genes as well as ileal PepT1 and L-FABP genes in challenged broilers (P < 0.05). In conclusion, xylanase supplementation of wheat-based diets improved FCR and AME in birds irrespective of C. perfringens infection and elevated apparent ileal digestibility of CP and mRNA expression of nutrient transporters in challenged birds.

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“…There might also be a short-term time scale of regulation by dietary Na + , just as there is a short-term regulation by dietary carbohydrate. Four hours after drinking 150 mM Na Cl, intestinal glucose transport increased markedly in chickens previously fed a low-salt diet, and glucose-uptake rates equalled those in chickens consuming a high-salt diet [64]. This rapid response suggests that the target of regulatory signals are mature enterocytes.…”

Section: Effects Of Changes In Levels Of Other Dietary Constituentsmentioning

confidence: 91%

Dietary and developmental regulation of intestinal sugar transport

Ferraris

2001

Biochemical Journal

219

124

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The Na+-dependent glucose transporter SGLT1 and the facilitated fructose transporter GLUT5 absorb sugars from the intestinal lumen across the brush-border membrane into the cells. The activity of these transport systems is known to be regulated primarily by diet and development. The cloning of these transporters has led to a surge of studies on cellular mechanisms regulating intestinal sugar transport. However, the small intestine can be a difficult organ to study, because its cells are continuously differentiating along the villus, and because the function of absorptive cells depends on both their state of maturity and their location along the villus axis. In this review, I describe the typical patterns of regulation of transport activity by dietary carbohydrate, Na+ and fibre, how these patterns are influenced by circadian rhythms, and how they vary in different species and during development. I then describe the molecular mechanisms underlying these regulatory patterns. The expression of these transporters is tightly linked to the villus architecture; hence, I also review the regulatory processes occurring along the crypt-villus axis. Regulation of glucose transport by diet may involve increased transcription of SGLT1 mainly in crypt cells. As cells migrate to the villus, the mRNA is degraded, and transporter proteins are then inserted into the membrane, leading to increases in glucose transport about a day after an increase in carbohydrate levels. In the SGLT1 model, transport activity in villus cells cannot be modulated by diet. In contrast, GLUT5 regulation by the diet seems to involve de novo synthesis of GLUT5 mRNA synthesis and protein in cells lining the villus, leading to increases in fructose transport a few hours after consumption of diets containing fructose. In the GLUT5 model, transport activity can be reprogrammed in mature enterocytes lining the villus column. Innovative experimental approaches are needed to increase our understanding of sugar transport regulation in the small intestine. I close by suggesting specific areas of research that may yield important information about this interesting, but difficult, topic.

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Effects of resalination on intestinal glucose transport in chickens adapted to low Na+ intakes

Cited by 7 publications

References 11 publications

Effects of an early lipopolysaccharide challenge on growth and small intestinal structure and function of broiler chickens

Effects of an early lipopolysaccharide challenge on growth and small intestinal structure and function of broiler chickens

Xylanase supplementation of a wheat-based diet improved nutrient digestion and mRNA expression of intestinal nutrient transporters in broiler chickens infected with Clostridium perfringens

Dietary and developmental regulation of intestinal sugar transport

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