Effects of resveratrol and methylprednisolone on biochemical, neurobehavioral and histopathological recovery after experimental spinal cord injury

Abstract

Aim: To investigate the neuroprotective effect of resveratrol in an experimental spinal cord injury (SCI) model in rats. Methods: Male Wistar albino rats weighing 200-250 g were randomized into six groups. Weight-drop trauma was performed for SCI. Group 1 underwent laminectomy alone. Group 2 underwent laminectomy followed by SCI. Groups 3, 4, 5, and 6 underwent laminectomy followed by SCI and received resveratrol (100 mg/kg), methylprednisolone (MP) (30 mg/kg), resveratrol (100 mg/kg) plus MP (30 mg/kg), and ethanol (2%), respectively. The rats were divided into two subgroups for biochemical analysis (killed at 24 h after surgery) and for neurobehavioral and histopathological evaluation (killed at 6 weeks after surgery). Posttraumatic neurological recovery after surgery was recorded weekly. Results: Groups 3 and 5 revealed significantly lower malondialdehyde, nitric oxide, xanthine oxidase, and higher glutathione levels than group 4 (P<0.05). Neurological recovery rates were significantly better in groups 3 and 5 than group 4 (P<0.05). When spinal trauma size ratios were compared, there was no significant difference between treatment groups. Conclusion: Resveratrol treatment revealed better biochemical recovery in the acute stage of trauma than MP treatment. Although resveratrol and combined treatment revealed better neurobehavioral recovery than MP treatment; resveratrol, MP, and combined treatment modalities improved histopathological recovery at the same level in the final stage of the experiment. Future studies involving different doses of resveratrol and different doses combinations with MP could promise better results as each drug has a different anti-oxidative mechanism of action.

Does dexmedetomidine reduce secondary damage after spinal cord injury? An experimental study

“…MDA is an important and the most commonly used indicator of lipid peroxidation and its level increases in tissues when they are exposed to oxidative stress. Numerous studies have postulated that MDA is significantly increased in animals exposed to traumatic SCI [3,4,8]. The results presented in this study have also indicated that lipid peroxidation increases in all blood, CSF, spinal cord tissues of the rabbits.…”

“…Thus, prevention of lipid peroxidation is important for attenuation of secondary damage. Some neuroprotective agents with antioxidant activity have been investigated in traumatic SCI and some of them have been found useful [3,22]. Due to neuroprotective [10,23] property, we used the dexmedetomidine in SCI and found that it only and slightly reduced lipid peroxidation in CSF and spinal cord tissue of the rabbits.…”

“…Several pharmacological agents are used against secondary injury after experimental spinal cord trauma. Beneficial effects of melatonin [31], resveratrol [3], etomidate [11], magnesium sulfate [27] and sodium channel blockers mexiletine, phenytoin and riluzole [4] have been shown in traumatic SCI in rodents.…”

Efficiacy Of Resveratrol And Quercetin After Experimental Spinal Cord İnjury

“…[25] It was determined that PON-1 and AE activity were significantly lower in patients with multiple traumas ,and those parameters may be beneficial when severity of trauma and potential efficiency of treatment are examined. In the current study, significantly higher serum and tissue TAS and PON-1 activity levels found in post-SCI resveratrol group relative to SCI and post-SCI DMSO groups are consistent with findings of Ates et al (2006;2007) indicating increased glutathione peroxidase level in rats given resveratrol after SCI and head trauma. [13,25] Elmali et al demonstrated that resveratrol significantly reduced MDA level in ischemia/reperfusion damage of skeletal muscle.…”

“…[13,25] Elmali et al demonstrated that resveratrol significantly reduced MDA level in ischemia/reperfusion damage of skeletal muscle. [26] It was also observed in the current study that TAS level, which indicates total antioxidative effect, increased in resveratrol treatment group. It can be speculated that effect derives from anti-oxidative effect and free radical scavenging property of resveratrol as determined in the literature, particularly studies conducted by Ates et al (2006;2007).…”