Effects of resveratrol in bull semen extender on post-thaw sperm quality and capacity for fertilization and embryo development

Assunção, C. M.; Mendes, V.; Brandão, Felipe Zandonadi; Batista, Ríbrio Ivan Tavares Pereira; Souza, Emiliane Nogueira de; Carvalho, Bruno Campos de; Quintão, C. C. R.; Raposo, Nádia Rezende Barbosa; Camargo, L. S. A.

doi:10.1016/j.anireprosci.2021.106697

Cited by 15 publications

(16 citation statements)

References 39 publications

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“…Still, they observed a reduction in different post-thawing kinetic parameters. Similarly, although Assunção et al (2021) found a positive effect of adding 50 μM of resveratrol on sperm motility and kinetics, our results were contrary for most of the same parameters. Therefore, it may be necessary to consider aspects other than concentration when using resveratrol as a supplement for semen freezing.…”

Section: Controlcontrasting

confidence: 99%

“…The addition of resveratrol in different concentrations for bovine semen freezing has had variable and unpredictable results on sperm quality. While Bucak et al (2015) found favourable results on TM with 1.0 mM of resveratrol, Assunção et al (2021) found no effect on TM. Still, they observed a reduction in different post-thawing kinetic parameters.…”

Section: Controlmentioning

confidence: 94%

“…However, the use of resveratrol in the cryopreservation of bovine semen has had variable and unpredictable effects on sperm quality. The result is that equal concentrations have shown both favourable and adverse effects on sperm motility and kinetics (Assunção et al., 2021; Bucak et al., 2015). Therefore, it is necessary to consider aspects other than the concentration of this antioxidant, which could modify its effect on sperm conservation.…”

Section: Introductionmentioning

confidence: 99%

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Quality and redox state of bovine sperm cryopreserved with resveratrol use of resveratrol in bovine semen

Correa,

Ceballos,

Rojano

et al. 2024

Reprod Domestic Animals

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The use of antioxidants for semen preservation prevents oxidative damage caused by reactive oxygen species (ROS). One of the most promising natural antioxidants is resveratrol, a phytoalexin derived from plants, grapes, berries, peanuts and red wine. To evaluate the effect of resveratrol on the quality and redox status of cryopreserved bovine semen. Five bulls were subjected to electroejaculation to obtain 15 ejaculates. Each ejaculate was extended with a tris‐egg yolk‐glycerol‐based medium and divided into six aliquots supplemented with 0 (control), 10, 20, 30, 40 and 50 μM of resveratrol. Semen was frozen with liquid nitrogen vapours. Post‐thawing, motility and kinetics were evaluated using a computer‐assisted sperm analysis (CASA) system, membrane integrity using the hypoosmotic test (HOST), morphology by staining with eosin–nigrosin, sperm vitality by fluorescence microscopy with the SYBR14/IP probes. Total antioxidant capacity (TAC) was evaluated using the ABTS•+ assay and ROS was evaluated using spectrofluorimetry with the H2DCFDA probe. For the statistical analysis linear models were adjusted and means were compared using the Tukey test. All concentrations of resveratrol reduced post‐thawed motility and kinetics of sperm. Supplementation with 40 and 50 μM of resveratrol reduced sperm kinetics, and between 30 and 50 μM of resveratrol alterations in the sperm membrane and morphology were observed. However, using resveratrol at 50 μM increased TAC and at 20 μM, it reduced ROS production of cryopreserved bovine semen. Resveratrol appears to have a dose‐dependent effect in which higher doses produce greater sperm alterations, however, it can increase semen TAC during freezing. It is concluded that resveratrol can increase antioxidant capacity and reduce ROS production in cryopreserved bovine semen. However, its use between 10 and 50 μM reduces post‐thawing semen quality.

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Section: Controlcontrasting

confidence: 99%

Section: Controlmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Quality and redox state of bovine sperm cryopreserved with resveratrol use of resveratrol in bovine semen

Correa,

Ceballos,

Rojano

et al. 2024

Reprod Domestic Animals

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“…Indeed, the damage caused by ROS could be irreversible, regardless of the amount of antioxidants secreted, making it a tedious task to accurately assess sperm quality and fertility capacities. For this reason several studies have shown the role of supplementation of different antioxidants, such as melatonin, resveratrol, and catalase, on the freezing and/or thawing media [ 52 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Relationships between Biomarkers of Oxidative Stress in Seminal Plasma and Sperm Motility in Bulls before and after Cryopreservation

Vigolo

Giaretta

Dalt

et al. 2022

Animals

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This study aimed at evaluating the relationship between biomarkers of oxidative stress (OS) in seminal plasma and sperm motility in bulls before and after cryopreservation. Three ejaculates per bull were collected from 20 young bulls. Each ejaculate was analyzed for motility before and after cryopreservation (by CASA), and the SP concentration of Advanced Oxidation Protein Products (AOPP), thiols, and carbonyl groups (CT) were examined. Then, based on their motility, the ejaculates were grouped into: high motility fresh (HMF), low motility fresh (LMF), high motility thawed (HMT), and low motility thawed (LMT) groups. Higher AOPP and thiol concentrations on SP were related (p < 0.05) to the higher LIN and BCF and lower ALH of fresh semen. In addition, AOPP and thiols were significantly higher in HMF than LMF. As a confirmation of this, the Receiver Operating Characteristic (ROC) curve analysis showed that AOPP and thiol concentrations in SP were able to discriminate between HMF and LMF ejaculates (Area Under the Curve of 71.67% and 72.04%, respectively). These observations give an alternative perspective on the relationship between sperm motility and the OS parameters of SP, which need further investigations.

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“…Accordingly, RES has been extensively studied as a suitable alternative supplement to cryopreservation media, primarily thanks to its ability to prevent oxidative damage to biomolecules critical for the sperm survival. An improved post thaw sperm motility, membrane stability and mitochondrial activity has been reported in human [ 56 ], bovine [ 57 ], ram [ 58 ] and boar spermatozoa [ 59 ] exposed to RES during the freezing and thawing process. Nevertheless, dose-dependent effects of RES have been frequently observed during sperm cryopreservation as well.…”

Section: Resveratrolmentioning

confidence: 99%

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

et al. 2021

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Emerging evidence from in vivo as well as in vitro studies indicates that natural biomolecules may play important roles in the prevention or management of a wide array of chronic diseases. Furthermore, the use of natural compounds in the treatment of male sub- or infertility has been proposed as a potential alternative to conventional therapeutic options. As such, we aimed to evaluate the effects of selected natural biomolecules on the sperm production, structural integrity, and functional activity. At the same time, we reviewed their possible beneficial or adverse effects on male reproductive health. Using relevant keywords, a literature search was performed to collect currently available information regarding molecular mechanisms by which selected natural biomolecules exhibit their biological effects in the context of male reproductive dysfunction. Evidence gathered from clinical trials, in vitro experiments and in vivo studies suggest that the selected natural compounds affect key targets related to sperm mitochondrial metabolism and motion behavior, oxidative stress, inflammation, DNA integrity and cell death. The majority of reports emphasize on ameliorative, stimulating and protective effects of natural biomolecules on the sperm function. Nevertheless, possible adverse and toxic behavior of natural compounds has been indicated as well, pointing out to a possible dose-dependent impact of natural biomolecules on the sperm survival and functionality. As such, further research leading to a deeper understanding of the beneficial or adverse roles of natural compounds is necessary before these can be employed for the management of male reproductive dysfunction.

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Effects of resveratrol in bull semen extender on post-thaw sperm quality and capacity for fertilization and embryo development

Cited by 15 publications

References 39 publications

Quality and redox state of bovine sperm cryopreserved with resveratrol use of resveratrol in bovine semen

Quality and redox state of bovine sperm cryopreserved with resveratrol use of resveratrol in bovine semen

Relationships between Biomarkers of Oxidative Stress in Seminal Plasma and Sperm Motility in Bulls before and after Cryopreservation

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

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