Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells

Abstract

Background and objectives: Cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathogenesis of atopic diseases such as atopic dermatitis, allergic rhinitis, and asthma. Resveratrol (RSV) exerts various pharmacological effects such as antioxidant, anti-inflammatory, neuroprotective, and anticancer. Although, it has been verified the beneficial effects of RSV on various subjects, the effect of RSV on thymic stromal lymphopoietin (TSLP) regulation has not been elucidated. Materials and Methods: Here, we examined how RSV regulates TSLP in HMC-1 cells. Enzyme-linked immunosorbent assay, real-time polymerase chain reaction, Western blotting, and calcium assay were performed to evaluate the effect of RSV. Results: TSLP production and mRNA expression were reduced by RSV. RSV down-regulated nuclear factor-κB activation, IκBα phosphorylation as well as activation of receptor-interacting protein2 and caspase-1 in HMC-1 cells. In addition, RSV treatment decreased the up-regulation of intracellular calcium in HMC-1 cells. Conclusions: These results suggest that RSV might be useful for the treatment of atopic diseases through blocking of TSLP.

Resveratrol Treatment Prevents Increase of Mast Cells in Both Murine OVA Enteritis and IL-10−/− Colitis

“…In addition, these levels are below the highest commercially available single dose of about 500 mg per tablet or capsule [100]. In vitro concentrations of resveratrol are also varying from low of 0.03 µM [20] to high doses of 100-200 µM [19], depending on the in vitro models used. The high metabolism and excretion of resveratrol [87] leads to low physiological concentrations (50 nM-2 µM), so that the in vitro concentrations used cannot be reached physiologically by the consumption of resveratrol in food [93].…”

“…Mast cells (MC) are key effector cells of type I allergy and release pro-inflammatory mediators such as pre-stored histamine or de novo synthesized cytokines and lipid mediators in response to IgE-dependent stimulation via the FcεRI receptor crosslinking by antigens [18]. The role of MC and their mediators was numerously shown not only in vitro in different MC models [19][20][21] or in vivo in several allergic conditions [22][23][24] but also in humans [25][26][27]. Consequently, MC play an important role in gastrointestinal disorders due to food allergens [28].…”

Resveratrol Attenuates Mast Cell Mediated Allergic Reactions: Potential for Use as a Nutraceutical in Allergic Diseases?

“…Furthermore, Moon et al. [ 46 ] and Kang et al. [ 47 ] reported a reduction in mRNA expression of proinflammatory mediators like thymic stromal lymphopoietin (TSLP) by using human mast cell line (HMC)‐1, which was stimulated with phorbol‐12‐myristate 13‐acetate (PMA) and calcium ionophore A23187 after pretreatment with RESV.…”

“…[ 47 ] Following the treatment with 3 µM of RESV intracellular calcium levels were reduced resulting in a decreased production of receptor interacting protein (RIP) 2/caspase‐1, which inhibited the activation of NF‐κB or the phosphorylation of IκBα by about 50%. [ 46 ] The authors assumed that this effect was responsible for the reduced TSLP production by RESV. [ 46 ] As described above, the inhibition of NF‐κB probably leads to an attenuation of allergic reactions by the decreased synthesis of other proinflammatory cytokines, too.…”

“…[ 46 ] The authors assumed that this effect was responsible for the reduced TSLP production by RESV. [ 46 ] As described above, the inhibition of NF‐κB probably leads to an attenuation of allergic reactions by the decreased synthesis of other proinflammatory cytokines, too. Furthermore, Kang et al.…”