Effects of riboflavin and AQS as electron shuttles on U(<scp>vi</scp>) reduction and precipitation by<i>Shewanella putrefaciens</i>

Wang, Pingping; Dong, Faqin; Wang, Xuhui; Liu, Mingxue; Nie, Xiaoqin; Zhou, Lei; Huo, Tingting; Zhang, Wei; Wei, Hongfu

doi:10.1039/c8ra05715j

Cited by 11 publications

(4 citation statements)

References 45 publications

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“…Mutations in the cytochrome c biosynthesis pathway in Photobacterium leiognathi can decrease the intensity of bioluminescence or is present in the bioluminescence pathway in the yellow‐emitting Vibrio fischeri Y‐1 . S. woodyi can secrete redox mediators such as flavins and quinones that can be reused through multiple redox cycles . Shewanella sp.…”

Section: Resultsmentioning

confidence: 99%

Soluble electron acceptors affect bioluminescence from Shewanella woodyi

et al. 2019

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Shewanella woodyi cultures were used to correlate bioluminescence intensity with changes in the electrochemical potential of a saltwater medium using soluble electron acceptors. A relationship between the concentration of NaNO 3 or CoCl 2 to bioluminescence intensity was confirmed using aerobic cultures of S. woodyi at 20 C with glucose as the sole carbon source. In general, increasing the concentration of nitrate or Co(II) reduced the bioluminescence per cell, with complete luminescence being repressed at ≥5 mM nitrate and ≥0.5 mM Co(II). Results from cell viability fluorescent staining concluded that increasing the concentration of Co(II) or nitrate did not affect the overall viability of the cells when compared with cultures lacking Co(II) or nitrate.These data show that potentials of <0.2 V vs Normal Hydrogen Electrode (NHE) repress the luminescence from the cells, but the exact mechanism is unclear. Our results indicated that the luminescence intensity from S. woodyi could be systematically reduced using these two soluble electron acceptors, making S. woodyi a potential model bacterium for whole-cell luminescence bioelectrochemical sensor applications. K E Y W O R D Sbioluminescence, extracellular electron transfer, redox potential, Shewanella

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Section: Resultsmentioning

confidence: 99%

Soluble electron acceptors affect bioluminescence from Shewanella woodyi

et al. 2019

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“…Several research groups, including ourselves, investigated the interaction of UO 2 2+ with S. putrefaciens. 67,68 However, under aerobic conditions, it remains unknown how the U(VI)-phosphate mineralization mediated by S. putrefaciens is affected by organic matter like DFO. As UO 2 2+ and Fe 3+ ions have similar metal toxicity in aqueous solutions, 47 it is essential to find out whether geochemical effects of DFO on phosphate mediated by S. putrefaciens go beyond their traditional functions as ironcomplexed ligands or not.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Several research groups, including ourselves, investigated the interaction of UO 2 2+ with S. putrefaciens. , However, under aerobic conditions, it remains unknown how the U(VI)-phosphate mineralization mediated by S. putrefaciens is affected by organic matter like DFO.…”

Section: Introductionmentioning

confidence: 99%

Chelating Effect of Siderophore Desferrioxamine-B on Uranyl Biomineralization Mediated by Shewanella putrefaciens

Lu,

Zhang,

Cheng

et al. 2024

Environ. Sci. Technol.

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In contaminated water and soil, little is known about the role and mechanism of the biometabolic molecule siderophore desferrioxamine-B (DFO) in the biogeochemical cycle of uranium due to complicated coordination and reaction networks. Here, a joint experimental and quantum chemical investigation is carried out to probe the biomineralization of uranyl (UO 2 2+ , referred to as U(VI) hereafter) induced by Shewanella putrefaciens (abbreviated as S. putrefaciens) in the presence of DFO and Fe 3+ ion. The results show that the production of mineralized solids {hydrogen−uranium mica [H 2 (UO 2 ) 2 (PO 4 ) 2 •8H 2 O]} via S. putrefaciens binding with UO 22+ is inhibited by DFO, which can both chelate preferentially UO 2 2+ to form a U(VI)-DFO complex in solution and seize it from U(VI)-biominerals upon solvation. However, with Fe 3+ ion introduced, the strong specificity of DFO binding with Fe 3+ causes re-emergence of biomineralization of UO 2 2+ {bassetite [Fe(UO 2 ) 2 (PO 4 ) 2 •8(H 2 O)]} by S. putrefaciens, owing to competitive complexation between Fe 3+ and UO 2 2+ for DFO. As DFO possesses three hydroxamic functional groups, it forms hexadentate coordination with Fe 3+ and UO 2 2+ ions via these functional groups. The stability of the Fe 3+ -DFO complex is much higher than that of U(VI)-DFO, resulting in some DFO-released UO 2 2+ to be remobilized by S. putrefaciens. Our finding not only adds to the understanding of the fate of toxic U(VI)-containing substances in the environment and biogeochemical cycles in the future but also suggests the promising potential of utilizing functionalized DFO ligands for uranium processing.

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“…21 According to new data, a large number of researchers have applied ESs to control environmental pollution such as that caused by uranium and the reduction of Fe and dichromate. 22,[24][25][26][27][28][29] In addition, electron transfer can promote microbial metabolism. 30 In anaerobic microbial communities, organic matter can be degraded.…”

Section: Introductionmentioning

confidence: 99%

Effects and mechanism of riboflavin on the growth ofAlcaligenes faecalisunder bias conditions

Chen

Liu

et al. 2019

RSC Adv.

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Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation byShewanella putrefaciens

Abstract: Understanding the mechanisms for electron shuttles (ESs) in microbial extracellular electron transfer (EET) is important in biogeochemical cycles, bioremediation applications, as well as bioenergy strategies.

Cited by 11 publications

References 45 publications

Soluble electron acceptors affect bioluminescence from Shewanella woodyi

Soluble electron acceptors affect bioluminescence from Shewanella woodyi

Chelating Effect of Siderophore Desferrioxamine-B on Uranyl Biomineralization Mediated by Shewanella putrefaciens

Effects and mechanism of riboflavin on the growth ofAlcaligenes faecalisunder bias conditions

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