Effects of Root Extract of Ruellia tuberosa L. on Kidneys of Diabetic Rats

Abstract

The aim of this research was to study the antidiabetic activity of root extract of Ruellia tuberosa L. on rats (Rattus novergicus) induced by multiple low doses of streptozotocin. The parameters investigated were blood glucose levels, protease activities, and the histopathologic profiles of the kidneys. Three groups of rats, i.e. control, diabetic, and treatment, were used. R. tuberosa L. extracts were given orally for treatment at a dose of 250 mg/kg body weight per day for 21 consecutive days. The induced diabetes group treated with root extract of R. tuberosa L. (group III) had 60.3% lower glucose levels (119 mg/dL) compared to the group with induced diabetes only. Moreover, antidiabetic activity of R. tuberosa L extract was evidenced by the 55% lower protease activity in the treatment group compared to the untreated diabetic group. Finally, a positive effect from root extract of R. tuberosa L. was shown from the less damaged renal glomerulus and smaller gap between extracellular matrices and epithelium cells in the histopathologic profiles of the kidneys in the extract treatment group. These results show the potential of root extract of R. tuberosa L. as a natural product anti-diabetic agent.

Ruellia tuberosa L. Extract Improves Histopathology and Lowers Malondialdehyde Levels and TNF Alpha Expression in the Kidney of Streptozotocin-Induced Diabetic Rats

“…After adaptation, rats were divided into five groups ( n = 5 in each group): control (group 1); diabetic (group 2); diabetic that treated with 250 mg/kg body weight extracts per day (group 3); diabetic that treated with 375 mg/kg body weight extracts per day (group 4); and diabetic that treated with 500 mg/kg body weight extracts per day (group 5). The extract doses were chosen based on the doses applied to our previous study [ 17 ]. Diabetes mellitus was induced by STZ injection intraperitoneally with 20 mg/kg body weight in 100 mL of citrate buffer for five days consecutively.…”

“…had shown antidiabetic activity through in vivo studies [ 16 , 17 ]. These effects included decreasing blood glucose concentrations, lowering levels of MDA, and showing improvements on the kidney histopathological profiles [ 16 , 17 ]. Phytochemical examination of this study showed that n -hexane R. tuberosa L .…”

“…Phytochemical examination of this study showed that n -hexane R. tuberosa L . root extracts containing triterpenoid compounds [ 17 ]. Furthermore, we have conducted an in vivo study on R. tuberosa L .…”

Molecular Docking Approach of Potential Alpha-glucosidase Inhibitors from Extracts Compounds of R. tuberosa L

“…The current study is investigating the activity from secondary metabolites compounds contained in R. tuberosa L. root extracts using in silico molecular docking approach. Based on our previous studies, R. tuberosa L. mostly contained flavonoids and phytosterol compounds [15,16]. In addition, the R. tuberosa L. hydroethanolic extracts have showed anti-diabetic activity in an in-vivo study [17][18][19].…”

Histopathological Profiles of Rats (Rattus norvegicus) Induced with Streptozotocin and Treated with Aqueous Root Extracts of Ruellia tuberosa L.

“…Based on our previous studies, n-hexane root extracts from R. tuberosa L. have activity as an antidiabetic drug in rat diabetic models [10]. ese results were shown by blood glucose reduction, MDA level decrease, and kidney histopathological profile recovery [10]. A qualitative test has demonstrated that triterpenoid compounds were present in the n-hexane R. tuberosa L. root extracts.…”