Effects of rosuvastatin on cardiovascular morphology and function in an ApoE-knockout mouse model of atherosclerosis

Grönros, Julia; Wikström, Johannes; Brandt-Eliasson, Ulla; Forsberg, Gun-Britt; Behrendt, Margareta; Hansson, Göran; Gan, Li‐Ming

doi:10.1152/ajpheart.00133.2008

Cited by 28 publications

(21 citation statements)

References 55 publications

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“…Studies (28,32) in mice and rats using CFVR have shown that coronary artery function is reduced in atherosclerosis, probably depending on both microvascular dysfunction and stenosis related to the disease. In addition, CFVR can be improved by statin treatment in atherosclerotic mice (9) or voluntary exercise in hypertensive rats (11). This highlights the importance of measuring in vivo coronary artery function in preclinical and clinical studies.…”

Section: Discussionmentioning

confidence: 99%

Arginase inhibition restores in vivo coronary microvascular function in type 2 diabetic rats

Grönros¹,

Jung²,

Lundberg

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

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Nitric oxide (NO) is crucial for maintaining normal endothelial function and vascular integrity. Increased arginase activity in diabetes might compete with NO synthase (NOS) for their common substrate arginine, resulting in diminished production of NO. The aim of this study was to evaluate coronary microvascular function in type 2 diabetic Goto-Kakizaki (GK) rats using in vivo coronary flow velocity reserve (CFVR) and the effect of arginase inhibition to restore vascular function. Different groups of GK and Wistar rats were given vehicle, the arginase inhibitor N(ω)-hydroxy-nor-l-arginine (nor-NOHA), l-arginine, and the NOS inhibitor N(G)-monomethyl -l-arginine (l-NMMA). GK rats had impaired CFVR compared with Wistar rats (1.31 ± 0.09 vs. 1.87 ± 0.05, P < 0.001). CFVR was restored by nor-NOHA treatment compared with vehicle in GK rats (1.71 ± 0.13 vs. 1.23 ± 0.12, P < 0.05) but remained unchanged in Wistar rats (1.88 ± 0.10 vs. 1.79 ± 0.16). The beneficial effect of nor-NOHA in GK rats was abolished after NOS inhibition. CFVR was not affected by arginine compared with vehicle. Arginase II expression was increased in the aorta and myocardium from GK rats compared with Wistar rats. Citrulline-to-ornithine and citrulline-to-arginine ratios measured in plasma increased significantly more in GK rats than in Wistar rats after nor-NOHA treatment, suggesting a shift of arginine utilization from arginase to NOS. In conclusion, coronary artery microvascular function is impaired in the type 2 diabetic GK rat. Treatment with nor-NOHA restores the microvascular function by a mechanism related to increased utilization of arginine by NOS and increased NO availability.

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Section: Discussionmentioning

confidence: 99%

Arginase inhibition restores in vivo coronary microvascular function in type 2 diabetic rats

Grönros¹,

Jung²,

Lundberg

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

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“…High resolution UBM technology has been widely used to detect atherosclerotic plaques in animal models [25, 26]. Compared with the traditional study of atherosclerotic plaques, there are several unique advantages inherent in UBM approaches.…”

Section: Discussionmentioning

confidence: 99%

Effects of Shenlian extract on experimental atherosclerosis in ApoE-deficient mice based on ultrasound biomicroscopy

Guo

Liu

Wang

et al. 2016

BMC Complement Altern Med

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BackgroundThis study directly and dynamically investigated the effects of SL extract (i.e., a combination of Radix Salviae miltiorrhizae and Andrographis paniculata extract) on plaque progression in vivo by high resolution ultrasound biomicroscopy (UBM).MethodsAn atherosclerosis model was established by placing a perivascular collar on the right common carotid artery in apolipoprotein E-deficient (ApoE-/-) mice. Thickness, plaque area and local blood flow were observed by UBM, pathological changes were observed by histochemical staining, and lipid levels were measured by respective commercially available kits.ResultsCompared with the model group, the SL extract groups showed reduced wall thickness of the aortic arch (GC: P = 0.001, P = 0.002, and P < 0.001; LC: P < 0.001, P < 0.001, and P < 0.001; BC: P = 0.027, P = 0.017, and P = 0.003; respectively), which presented with retarded plaque progression of the cartoid artery with concordantly increased blood flow (P = 0.002 and P < 0.001) as visualized in vivo by UBM. Histological analysis confirmed the reduction of carotid atherosclerosis.ConclusionsThe SL extract inhibited the formation of atherosclerotic plaques in an ApoE-/- mice model by UBM analysis, and did so by effects that ameliorated local blood flow and improved blood lipid levels.

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“…Microbubbles have been applied mainly as blood pool agents to enhance the echogenicity of the cardiac chambers during echocardiography for better delineation of the myocardial borders and for perfusion assessment of the myocardium [57]. …”

Section: Applicationsmentioning

confidence: 99%

Ultrasound Biomicroscopy in Small Animal Research: Applications in Molecular and Preclinical Imaging

Greco

Mancini

Gargiulo

et al. 2012

Journal of Biomedicine and Biotechnology

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Ultrasound biomicroscopy (UBM) is a noninvasive multimodality technique that allows high-resolution imaging in mice. It is affordable, widely available, and portable. When it is coupled to Doppler ultrasound with color and power Doppler, it can be used to quantify blood flow and to image microcirculation as well as the response of tumor blood supply to cancer therapy. Target contrast ultrasound combines ultrasound with novel molecular targeted contrast agent to assess biological processes at molecular level. UBM is useful to investigate the growth and differentiation of tumors as well as to detect early molecular expression of cancer-related biomarkers in vivo and to monitor the effects of cancer therapies. It can be also used to visualize the embryological development of mice in uterus or to examine their cardiovascular development. The availability of real-time imaging of mice anatomy allows performing aspiration procedures under ultrasound guidance as well as the microinjection of cells, viruses, or other agents into precise locations. This paper will describe some basic principles of high-resolution imaging equipment, and the most important applications in molecular and preclinical imaging in small animal research.

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Effects of rosuvastatin on cardiovascular morphology and function in an ApoE-knockout mouse model of atherosclerosis

Cited by 28 publications

References 55 publications

Arginase inhibition restores in vivo coronary microvascular function in type 2 diabetic rats

Arginase inhibition restores in vivo coronary microvascular function in type 2 diabetic rats

Effects of Shenlian extract on experimental atherosclerosis in ApoE-deficient mice based on ultrasound biomicroscopy

Ultrasound Biomicroscopy in Small Animal Research: Applications in Molecular and Preclinical Imaging

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