Effects of Royal Jelly and Bee Pollen on the Growth of Selected Probiotic Bacteria (Bf. animalis Spp. Lactis, L. acidophilus and L. casei)

Abstract

A b s t r a c t In this research article, the effects of bee pollen and royal jelly on the selected probiotic bacteria, as growth factors, were investigated. The probiotic cultures were activated in MRS broth at 37°C. Then, bee pollen and royal jelly (10 mg/100 μL, 25 mg/250 μL, 50 mg/500 μL, 75 mg/750 μL, and 100 mg/1000 μL) were added on the probiotic cultures in MRS broth and sampled at 0, 24, and 48 hours of incubation. The medias used for enumeration of the probiotic cultures were RCA (Reinforced Clostridial Agar) for Bf. animalis spp. lactis, MRS (deMann, Rogosa and Sharpe) Agar with D-sorbitol for Lb. acidophilus and MRS-Vancomycine Agar for Lb. casei. The lactic acid production by Lb. acidophilus, Lb. casei, and Bf. animalis spp. lactis, and acetic acid production by Bf. animalis spp. lactis, were determined to compare the bacterial proliferation. The probiotic cultures were mainly affected by the bee pollen and royal jelly during the first 24 hours. The changes observed in the number of probiotic counts between 24 and 48 hours were not significant, statistically (P<0.05). Generally, the probiotic bacterial counts increased parallel to the concentration of bee pollen or royal jelly up to 75mg, and remained unchanged above this concentration. In terms of lactic acid production and bacterial growth, the most significant growth was observed on Lb. acidophilus when bee pollen or royal jelly was added.

Bee Pollen Extracts: Chemical Composition, Antioxidant Properties, and Effect on the Growth of Selected Probiotic and Pathogenic Bacteria

“…According to Guldas [113], the probiotic bacterial counts increased with the concentration of bee pollen up to 75 mg during 24 h of cultivation (B. animalis with 8.09%, L. acidophilus with 8.70%, L. casei with 10%) and generally above this concentration remained unchanged. Adding bee pollen stimulated lactic acid production and bacterial growth, especially on L. acidophilus.…”

“…A recent study has shown that L. rhamnosus GG ATCC 53103 and L. acidophilus NRRLB 4495 better adapt to catechin and gallic, protocatechuic, and vanillic acids than the pathogenic bacteria tested [28]. According to Guldas [113], the probiotic bacterial counts increased with the concentration of bee pollen up to 75 mg during 24 h of cultivation (B. animalis with 8.09%, L. acidophilus with 8.70%, L. casei with 10%) and generally above this concentration remained unchanged. Adding bee pollen stimulated lactic acid production and bacterial growth, especially on L. acidophilus.…”

“…Adding bee pollen stimulated lactic acid production and bacterial growth, especially on L. acidophilus. The bee pollen also contains significant food sources for probiotic bacterial growth, such as glucose and fructose, B vitamins and derivates, and trace elements such as Fe, Cu, Zn, and Mn [113]. Besides the assessment of the antimicrobial effect of the BPE and soluble compounds of lactic strains on the adherence capacity to the sensitive cellular substrate of some pathogenic strains, it can be considered that the BPE, together with the soluble compounds of L. rhamnosus MF9 and E. faecalis 2M17, inhibited without exception the adherence capacity of the C. guillermondii and E. cloacae, compared to the control at 1:10 (Figures 7 and 8).…”

Royal Jelly as an Intelligent Anti-Aging Agent—A Focus on Cognitive Aging and Alzheimer’s Disease: A Review

“…At present, universally accepted biomarkers and preventive strategies of AD are quite limited [ 214 ]. Imaging studies state that an extremely long preclinical phase paves the way for the active symptomatic phase of AD.…”

“…Therefore, detecting and treating cognitive decline at an early stage is necessary to prevent the development of dementia [ 204 ]. Attention has been focused on the use of simple, low-cost interventions to manage modifiable risk factors for AD, such as metabolic dysfunctions (e.g., diabetes mellitus, hypertension, hyperlipidemia, and obesity), sedentary life style, and unhealthy diet [ 214 ]. Depressive disorders, especially treatment resistant ones, may represent another AD prevention target.…”