Effects of S-PRG filler eluate on MMP-1 and MMP-3 secretion by human gingival fibroblasts

Abstract

The aim of this study was to investigate the effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) filler eluate on Matrix metalloproteinase (MMP)-1 and MMP-3 secretion by human gingival fibroblasts (HGF). The S-PRG filler eluate contains 6 ions (F, Na, Al, B, Sr and Si) released from the S-PRG filler. The S-PRG filler eluate stimulation induced a slight secretion of MMP-1 and MMP-3 by HGF. It also enhanced the phosphorylation of p38 and ERK. The increase in MMP-1 and MMP-3 secretion by the inflammatory cytokine TNF-α was suppressed by the S-PRG filler eluate. TNF-α-induced increases in the phosphorylation of ERK were slightly enhanced by S-PRG filler eluate. These findings may prompt the development of new therapeutic agents for oral inflammation with materials composed of S-PRG filler eluate.

Effects of a co-stimulation with S-PRG filler eluate and muramyl dipeptide (MDP) on matrix metalloproteinase-1 production by human dental pulp fibroblast-like cells

“…Moreover, the activation of MAP kinase via NOD-2 was demonstrated in murine macrophages, in which p38, ERK 1/2, and JNK were phosphorylated after a stimulation with MDP 17) . We previously demonstrated that ERK 1/2 was involved in MMP-1 production by human gingival fibroblasts stimulated with S-PRG filler eluates 16) . Therefore, in the present study, we investigated changes in ERK1/2 phosphorylation upon a co-stimulation with MDP and S-PRG filler eluate.…”

“…Based on these findings, S-PRG filler eluate appears to endow pulp capping with several beneficial properties, leading to reparative dentin. We previously demonstrated that S-PRG filler eluate enhanced the production of MMPs by human gingival fibroblast cells 16) . MMP-1 levels were increased by MDP, resulting in the development of inflammation, such as pulpitis 7) .…”

“…We previously reported that S-PRG filler eluateactivated human gingival fibroblasts produced MMP-1 16) ; therefore, we herein investigated whether S-PRG filler eluate affected the production of MMP-1 by hDPFs. When cells were cultured in the presence of 0.01, 0.1, and 1% S-PRG filler eluate, the dose-dependent production of MMP-1 was observed.…”