Effects of salinity acclimation on the growth performance, osmoregulation and energy metabolism of the oriental river prawn, Macrobrachium nipponense (De Haan)

Abstract

In this study, we evaluated the growth, osmoregulation and energy metabolism of the oriental river prawn, Macrobrachium nipponense, reared during 6 weeks with different salinities (0, 8, 14 and 22 g/L). The results showed that the haemolymph osmolality of M. nipponense increased with an increase in ambient osmotic pressure; the isosmotic point was 490 mOs/kg H 2 O. The prawns showed a higher survival rate, weight gain rate and hepatopancreas index in salinity 14 g/L. Digestive enzymes were all affected by salinity, and the highest activities were observed in the salinity 14 g/L. The mRNA expression of Na + -K + -ATPase in gills and p53 in hepatopancreas were the highest in salinity 22 g/L. The expressions of heat shock protein 90 and glutathione S-transferase genes in hepatopancreas were significantly higher in the salinity 8 g/L. Lipid metabolism-related genes in hepatopancreas were significantly expressed in the salinity 14 g/L. The glucose-6-phosphatase gene in hepatopancreas was highly expressed in the salinity 8 and 22 g/L, and the expression of the ecdysone receptor gene in hepatopancreas was significantly higher in the salinity 14 g/L. The results showed that salinity 14 g/L could promote the growth of M. nipponense. However, higher salinity conditions may cause physiological damage, which provides a theoretical basis for brackish water culture of M. nipponense. K E Y W O R D Senergy metabolism, growth, Macrobrachium nipponense, osmotic pressure, salinity

Identifying Neuropeptide and G Protein-Coupled Receptors of Juvenile Oriental River Prawn (Macrobrachium nipponense) in Response to Salinity Acclimation

“…The salinity was gradually adjusted on the same day to reach the target salinity for each group: S0 = 0.4 (control group), S6 = 8 ± 0.2 (low salinity), S12 = 16 ± 0.2 (high salinity). Salinity and water quality were maintained as previously described (2), and the prawns provided with commercial feed (Zhejiang Tongwei Feed Group CO., Ltd) twice daily for 1 week at a ratio of 6-8% of their body weight.…”

“…Among them, the Macrobrachium nipponense is an economically important economic species in aquaculture, with a production of in excess of 250,000 tons and an output reaching 2 billion RMB per year in China ( 1 ). In the aquaculture industry, culturing seawater species for desalination and using freshwater crustacean species for saltwater acclimation are new trends ( 2 ). In the past two decades, large numbers of the genus Macrobrachium have invaded freshwater habitats from the ancestral marine environment, and have exhibited high adaptability to slightly brackish and freshwater habitats ( 3 – 5 ).…”

Long‐term low‐salinity stress affects growth, survival and osmotic related gamma‐aminobutyric acid regulation in the swimming crab Portunus trituberculatus

“…The salinity of the coastal aquaculture area can easily be affected by rainfall, especially after a typhoon (Xu, Liu, Xu, & Xu, ), also, the acclimation of seawater species for low‐salinity culture to improve the productivity under sub‐optimal salinity has become a new trend in the aquaculture industry (Huang et al, ; McGraw, Davis, Teichert‐Coddington, & Rouse, ; Resley, Webb, & Holt, ). Many marine species have an osmoregulatory capacity to resist such low salinity (Romano & Zeng, ).…”

Estudo bioquímico comparativo da (Na+ , K+)-ATPase branquial de Macrobrachium amazonicum (Heller, 1862) habitante de regiões continentais

“…O valor de osmolalidade da hemolinfa dos juvenis é menor que a dos adultos, lógica esta que se inverte em M. rosenbergii (Wilder et al, 1998 ;Singh, 1980;Sandifer et al, 1975;Armstrong et al, 1981). Considerando que a existência de um menor gradiente entre a osmolalidade do meio externo e da hemolinfa necessita menor energia (Augusto et al, 2007), aparentemente estas espécies se adaptaram de forma distinta ao longo da evolução provavelmente necessitando de um maior gasto energético em outras funções nos diferentes estágios ontológicos (Fabri, 2019, este trabalho (Wilder et al, 1998;Freire et al, 2003;Ordiano et al, 2005;Lucena, 2014;Huang et al, 2019). Um parâmetro que melhor reflete a adaptação dessas espécies à água doce é o valor do ponto isosmótico da hemolinfa, que será menor quanto melhor a adaptação da espécie à água doce (Mantel & Farmer, 1983 (Denne, 1968;Moreira et al, 1983;Freire et al, 2003;Augusto et al, 2007;Huang et al, 2019).…”

“…Quando expostos a altas salinidades os camarões palemonídeos hiperregulam, necessitando secretar Na + e Cl - (Péqueux, 1995;McNamara & Faria, 2012). Esta exposição causa alterações na expressão e na atividade da (Na + , K + )-ATPase e V(H + )-ATPase no tecido branquial, sendo estas alterações são espécie-dependente e muitas vezes também tempodependente, porém algumas espécies não apresentam alterações significativas (Mendonça et al, 2007;Belli et al, 2009;Faleiros et al, 2010;Leone et al, 2014;Lucena et al, 2015;Introdução________________________________________________________________43 Maraschi et al, 2015;Ituarte et al, 2016;Freire et al, 2018;Huang et al, 2019).…”