Effects of salinity and temperature on reproductivity and fatty acid synthesis in the marine rotifer Brachionus rotundiformis

Lee, Min Chul; Yoon, Dokyoung; Park, Jun Chul; Choi, Hyuntae; Shin, Kyung-Hoon; Hagiwara, Atsushi; Lee, Jae‐Seong; Park, Heum Gi

doi:10.1016/j.aquaculture.2021.737282

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…in P. littoralis (Boyen et al, 2020;Sahota et al, 2022;Werbrouck et al, 2017) and other primary consumers (Lee et al, 2017(Lee et al, , 2022Masclaux et al, 2012). These impacts should be considered, as…”

Section: Discussionmentioning

confidence: 99%

Functional characterization reveals a diverse array of metazoan fatty acid biosynthesis genes

et al. 2022

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Long‐chain (≥C20) polyunsaturated fatty acids (LC‐PUFAs) are physiologically important fatty acids for most animals, including humans. Although most LC‐PUFA production occurs in aquatic primary producers such as microalgae, recent research indicates the ability of certain groups of (mainly marine) invertebrates for endogenous LC‐PUFA biosynthesis and/or bioconversion from dietary precursors. The genetic pathways for and mechanisms behind LC‐PUFA biosynthesis remain unknown in many invertebrates to date, especially in non‐model species. However, the numerous genomic and transcriptomic resources currently available can contribute to our knowledge of the LC‐PUFA biosynthetic capabilities of metazoans. Within our previously generated transcriptome of the benthic harpacticoid copepod Platychelipus littoralis, we detected expression of one methyl‐end desaturase, one front‐end desaturase, and seven elongases, key enzymes responsible for LC‐PUFA biosynthesis. To demonstrate their functionality, we characterized eight of them using heterologous expression in yeast. The P. littoralis methyl‐end desaturase has Δ15/17/19 desaturation activity, enabling biosynthesis of α‐linolenic acid, eicosapentaenoic acid and docosahexaenoic acid (DHA) from 18:2 n−6, 20:4 n−6 and 22:5 n−6, respectively. Its front‐end desaturase has Δ4 desaturation activity from 22:5 n−3 to DHA, implying that P. littoralis has multiple pathways to produce this physiologically important fatty acid. All studied P. littoralis elongases possess varying degrees of elongation activity for saturated and unsaturated fatty acids, producing aliphatic hydrocarbon chains with lengths of up to 30 carbons. Our investigation revealed a functionally diverse range of fatty acid biosynthesis genes in copepods, which highlights the need to scrutinize the role that primary consumers could perform in providing essential nutrients to upper trophic levels.

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Section: Discussionmentioning

confidence: 99%

Functional characterization reveals a diverse array of metazoan fatty acid biosynthesis genes

et al. 2022

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“…The fact that P. similis are cultured at 35 ppt can ensure the availability of the prey after being transferred to the same or close salinity; however, under these conditions, the growth of rotifers is lower, which can increase operating time and costs. A recent study suggests that the fatty acid composition of marine rotifers is better under their optimal culture conditions (Lee et al, 2022). In this context, we recommend that P. similis be cultured at optimal salinity conditions and subsequently transferred to the desired salinities to transfer essential nutrients to larvae.…”

Section: Discussionmentioning

confidence: 99%

Availability of the euryhaline rotifer Proales similis as prey after rapid salinity transfer

Rebolledo

Macías‐Velázquez

et al. 2022

Aquaculture Research

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In this work, we examined the effects of rapid changes in salinity on the availability (mobility and swimming) of the prey rotifer Proales similis. Rotifers were transferred as follows: 5 to 15, 25 and 35 (T1); 15 to 5, 25 and 35 (T2); 25 to 5, 15 and 35 (T3) and 35 to 5, 15 and 25 ppt (T4) during 1-240 min. Results showed that in T2 and T3, the percentage of mobile rotifers in the water column recovered up to 100% after 15 min. At T1 and T4, mobility regained slower at 35 and 5 ppt respectively. All individuals transferred at 15 and 25 ppt maintained their mobility above 93% after 15 min. In general, swimming speed ranged from 0.37 to 0.65 mm s −1 and was higher at 15 ppt and lower at 35 ppt. In all salinities, swimming increased with time and recovered better in T2 and T3. These data suggest that P. similis can acclimate to a wide range of salinity gradients in the short term (1-240 min). According to the literature and our results, P. similis has a greater tolerance to sudden salinity changes than brachionid rotifers used traditionally in fish larviculture.

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“…PUFAs could enhance the nutritional status of rotifers (Lee et al, 2022), which were the important factors that rotifers could be used as live feed in aquaculture (Lubzens, 1987; Lubzens et al, 1989). In this study, C18:2n6 and C18:3n‐3 were the dominant fatty acids of PUFAs.…”

Section: Discussionmentioning

confidence: 99%

“…for the growth of seafood seedlings and have characteristics such as small size and slow motility and can be easily preyed upon by aquatic animal larvae (Lubzens, 1987; Lubzens et al, 1989). In addition, rotifers have rapid reproduction and can be cultured at a relatively high density, being possible to develop large‐scale intensive cultivation of rotifers (Lee et al, 2022). Therefore, rotifers are regarded as an ideal live food source for the larvae of economically important aquatic species, including fish, shrimp, crab, etc.…”

Section: Introductionmentioning

confidence: 99%

Effects of supply ratio of chicken manure extract and yeast solution on reproductivity and culture conditions of euryhaline rotifers (Brachionus plicatilis)

Zhu

et al. 2022

Aquaculture Research

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To establish the optimal ratios of chicken manure extract (CME) and suspending yeast solution (SYS) for rearing rotifers, the population density, population growth rate, fatty acid compositions of rotifers and the unionized ammonia (NH 3 -N) and total phosphorus (TP) concentrations in cultured water under different ratios of CME and SYS (BG, EG1, EG2, EG3, EG4 and EG5) were investigated. The results showed that the population density and population growth rate of rotifers in EG2 (4 ml•L −1 CME: 0.3 ml•L −1 SYS) were the highest and reached 387.33 ± 12.34 and 0.57 ± 0.01 ind.•ml −1 , respectively. In addition, Pearson's correlation analysis indicated that the proportions of polyunsaturated fatty acids (PUFAs), n-3PUFAs, n-6PUFAs and long-chain PUFAs in rotifers were positively correlated with the content of CME (p < 0.05). Furthermore, NH 3 -N and TP in the cultured water decreased with the SYS contents increasing.Compared with EG5, the concentrations of NH 3 -N and TP in EG2 were reduced by 58.83 ± 0.05% and 72.52 ± 0.17%, respectively. These results indicated that both the population growth and fatty acid composition of rotifers, and the concentrations of NH 3 -N and TP in the cultured water were significantly affected by the ratios of CME and SYS. And EG2 was optimal for rearing the rotifer.

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Effects of salinity and temperature on reproductivity and fatty acid synthesis in the marine rotifer Brachionus rotundiformis

Cited by 13 publications

References 37 publications

Functional characterization reveals a diverse array of metazoan fatty acid biosynthesis genes

Functional characterization reveals a diverse array of metazoan fatty acid biosynthesis genes

Availability of the euryhaline rotifer Proales similis as prey after rapid salinity transfer

Effects of supply ratio of chicken manure extract and yeast solution on reproductivity and culture conditions of euryhaline rotifers (Brachionus plicatilis)

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