Effects of salinity on growth and compatible solutes of callus induced from Populus euphratica

Zhang, F.; Yang, Ying-Gen; He, Wei‐Ming; Zhao, Xue Qiang; Zhang, L. X.

doi:10.1079/ivp2004546

Cited by 49 publications

(41 citation statements)

References 26 publications

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“…The accessions A-23 had the maximum relative fresh weight of callus (6.33 g) under T 1 , A-48 under T 2 (8.66 g) and A-79 under T 3 (7g).The lowest value of relative fresh weight of callus was observed in the accession CM-621 (-6.21g, -0.33 g, 0 g) under T 1 , T 2 and T 3 respectively. The reduction in callus growth may be due to the fewer uptakes of nutrients under limited water stress conditions [35] and consequently the injury of cell membrane which is also measured in thus study [36], [37].…”

Section: Methodsmentioning

confidence: 99%

In Vitro Selection of the Sunflower (Helianthus Annuus L.) Accessions under the Polyethylene Glycol Mediated Drought Stress Conditions

2016

IJAAEE

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Abstract-Responses of the sunflower accessions toPolyethylene glycol (PEG-6000) mediated drought stress was examined in vitro culture under triplicate completely randomized deign. Three drought treatments i.e. T 1 = Zero (Control), T 2 = -0.35 MPa and T 3 = -1.33 MPa were developed. Seeds of the accession were grown on half strength MS media and three explants (leaf, stem, coleoptiles) were used for the callus culture. Stress was applied at the time of the media preparation for callus. Fifty petri plates of each accession were prepared and divided into two groups (Group I, Group II) containing 25 plated each. Petri plates of Group I were maintained for callus culture while of Group II were used for regeneration. Data were recorded from these groups on the traits i.e. relative fresh weight (g), proline content (µg), relative membrane leakage (µg), total soluble sugar contents (µg), coleoptiles length (mm), root length (mm), root count, fresh and dry root weight (g) and fresh and dry shoot weight (g). On the basis of the above parameters, the accessions A-23 and HBRS-1 were drought tolerant and 017577 as drought sensitive. In addition, the results of this study illustrate the potential of using in vitro culture for evaluation of drought tolerance at cellular level.

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Section: Methodsmentioning

confidence: 99%

In Vitro Selection of the Sunflower (Helianthus Annuus L.) Accessions under the Polyethylene Glycol Mediated Drought Stress Conditions

2016

IJAAEE

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“…In vitro culture is a valid and rapid tool for studying the behavior of plants in response to salt stress, while other factors (nutrients, lighting, temperature) are held constant and controlled in an optimal manner. This approach has been successfully used for the evaluation of salt stress in brassica [8], tomato [9][10][11][12][13][14][15][16], mulberry [17,18], potato [19], rice [20][21][22], poplar [23,24], strawberry [25], eucalyptus [26][27][28], grapevine [29,30], citrus [31,32], and pistacia [33].…”

Section: Introductionmentioning

confidence: 99%

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

et al. 2013

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In order to quickly and efficiently evaluate the salt tolerance of alfalfa, salinity tests were conducted on Medicago sativa L. var. australis, var. icon, var. loi, and var. gea, under in vitro conditions. Pregerminated seeds of four varieties were subjected to five different NaCl concentrations (0, 50, 100, 150, 200 mM). The influence of saline stress was estimated on the basis of survival percentage, growth parameters, and electrolyte leakage. The seedlings surviving on the medium enriched with salt at the highest concentration were presumed to be tolerant and represented the mother plants for the production of in vitro clones. In the following step, the clones were evaluated in vitro to confirm the salt tolerance. The influence of mild salt stress (75 mM NaCl) on the growth parameters of selected clones was examined. At the end of this trial, the proline accumulation and sodium content in alfalfa shoots were also quantified. The results suggest an increased level of proline promotes salt tolerance. Medicago sativa L. var. icon is highly tolerant in comparison with the other varieties tested. In vitro selection of M. sativa L. varieties on salt-containing media allowed us to obtain clones with increased salinity tolerance.

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“…To date, molecular studies of salt resistance in plants have centered on herbaceous species (Bohnert and Cushman 2000;Inan et al 2004), however, because of their long lifespan trees may have evolved adaptive strategies differing from those of annual plants, to survive long periods of stress (Ottow et al 2005). Thus, there is interest in studying salt resistance in trees, and P. euphratica appears to be one of the most promising model systems for such studies (Chen et al 2003;Gu et al 2004b;Zhang et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of PeSOS1: the putative Na+/H+ antiporter of Populus euphratica

Ding

Zhao

et al. 2007

Plant Mol Biol

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Populus euphratica is a salt-tolerant tree species growing in semi-arid saline areas. A Na(+)/H(+) antiporter gene was successfully isolated from this species through RACE cloning, and named PeSOS1. The isolated cDNA was 3665 bp long and contained a 3438 bp open reading frame that was predicted to encode a 127-kDa protein with 12 hypothetical transmembrane domains in the N-terminal part and a long hydrophilic cytoplasmic tail in the C-terminal part. The amino acid sequence of this PeSOS1 gene showed 64% identity with the previously isolated SOS1 gene from the glycophyte Arabidopsis thaliana. The level of protein expressed by PeSOS1 in the leaves of P. euphratica was significantly up-regulated in the presence of high (200 mM) concentrations of NaCl, while the mRNA level in the leaves remained relatively constant. Immunoanalysis suggested that the protein encoded by PeSOS1 is localized in the plasma membrane. Expression of PeSOS1 partially suppressed the salt sensitive phenotypes of the EP432 bacterial strain, which lacks the activity of the two Na(+)/H(+) antiporters EcNhaA and EcNhaB. These results suggest that PeSOS1 may play an essential role in the salt tolerance of P. euphratica and may be useful for improving salt tolerance in other tree species.

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Effects of salinity on growth and compatible solutes of callus induced from Populus euphratica

Cited by 49 publications

References 26 publications

In Vitro Selection of the Sunflower (Helianthus Annuus L.) Accessions under the Polyethylene Glycol Mediated Drought Stress Conditions

In Vitro Selection of the Sunflower (Helianthus Annuus L.) Accessions under the Polyethylene Glycol Mediated Drought Stress Conditions

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

Molecular characterization of PeSOS1: the putative Na+/H+ antiporter of Populus euphratica

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