Effects of salinity on the accumulation of hemocyte aggregates and bacteria in the gills of Callinectes sapidus, the Atlantic blue crab, injected with Vibrio campbellii

Ikerd, Jennifer L.; Burnett, Karen G.; Burnett, Louis E.

doi:10.1016/j.cbpa.2014.12.030

Cited by 17 publications

(6 citation statements)

References 38 publications

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“…The expression is higher in the gills, eyestalk, and hepatopancreas, and lowest in the muscle. The gills, eyestalk, and hepatopancreas are important immune organs of crustaceans (Rõszer, 2014;Ikerd et al, 2015). High PtP38 expression indicates that PtP38 may be related to the immune function of P. trituberculatus.…”

Section: Discussionmentioning

confidence: 99%

PtP38 May Increase the Immune Ability of Portunus trituberculatus Stimulated by LPS Imitating a Gram-Negative Bacterial Infection

Wei

Zhu

et al. 2021

Front. Mar. Sci.

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The P38 mitogen-activated protein kinase (MAPK) signal transduction pathway is widespread in organisms and plays important roles in immune activities. The infection mechanism of environmental gram-negative bacteria on crustaceans is an important scientific problem. In this study, the cDNA full-length sequence of Portunus trituberculatus P38 (PtP38) was cloned and its structure was analyzed by bioinformatics methods. To study the function of the PtP38 gene after a Gram-negative bacterial infection, we injected P. trituberculatus with LPS to activate the immune response instead of directly infecting with Gram-negative bacteria. With LPS stimulation, the expression of the PtP38 gene in different tissues increased significantly. At the same time, the expression of immune-related genes (ALF and crustin) in the hepatopancreas, activities of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), and inducible nitric oxide synthase (iNOS) enzymes], and expression of apoptosis-related genes (caspase2 and caspase3) were increased significantly. To further conform the function of PtP38 in the immune response, we injected P. trituberculatus with P38 inhibitor and subsequently injected with LPS. The results showed that the expression of immune-related genes was inhibited, the activity of antioxidant enzymes was decreased, and the expression of apoptosis-related genes were inhibited. Thus, we speculated that PtP38 may increase the immune ability by improving the expression of antimicrobial peptides, increasing the activity of oxidative stress-related enzymes, and promoting cell apoptosis in infected P. trituberculatus. This study also laid the foundation for further study of the P38 MAPK signaling pathway and immune mechanism of P. trituberculatus.

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Section: Discussionmentioning

confidence: 99%

PtP38 May Increase the Immune Ability of Portunus trituberculatus Stimulated by LPS Imitating a Gram-Negative Bacterial Infection

Wei

Zhu

et al. 2021

Front. Mar. Sci.

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“…the percentage of haemocytes containing endocytosed bacteria or latex beads in in vitro tests, or indirectly by measuring the production of ROS. Injected particles accumulate in the gills and can be eliminated together with haemocyte aggregates (Ikerd, Burnett & Burnett, 2015). When circulating haemocytes detect the presence of pathogens, foreign particles, or endogenous anomalous entities, they rapidly aggregate, entrapping the pathogens/particles.…”

Section: Phagocytes and Tlrs In Crustacean Immunity And Nanoparticle–...mentioning

confidence: 99%

Interaction between nanomaterials and the innate immune system across evolution

et al. 2023

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Interaction of engineered nanomaterials (ENMs) with the immune system mainly occurs with cells and molecules of innate immunity, which are present in interface tissues of living organisms. Immuno-nanotoxicological studies aim at understanding if and when such interaction is inconsequential or may cause irreparable damage. Since innate immunity is the first line of immune reactivity towards exogenous agents and is highly conserved throughout evolution, this review focuses on the major effector cells of innate immunity, the phagocytes, and their major sensing receptors, Toll-like receptors (TLRs), for assessing the modes of successful versus pathological interaction between ENMs and host defences. By comparing the phagocyte-and TLR-dependent responses to ENMs in plants, molluscs, annelids, crustaceans, echinoderms and mammals, we aim to highlight common recognition and elimination mechanisms and the general sufficiency of innate immunity for maintaining tissue integrity and homeostasis.

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“…Research has found that in intertidal crabs ( Macrophthalmus japonicus ) exposed to high osmotic environments, heat-excited proteins were involved in cellular homeostasis and immune defense in response to high exposure to high-salt environments [ 20 ]. A study on the impact of salinity on the immune defense of the Atlantic blue crab ( Callinectes sapidus ) found that a decrease in salinity resulted in increased blood cell aggregation and bacterial accumulation in the gill tissue of Callinectes sapidus [ 21 ]. In conclusion, crustaceans counteract changing salinity environments through the regulation of immune function.…”

Section: Introductionmentioning

confidence: 99%

Regulation and Response Mechanism of Acute Low-Salinity Stress during Larval Stages in Macrobrachium rosenbergii Based on Multi-Omics Analysis

Li,

Xu,

Shen

et al. 2024

IJMS

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Macrobrachium rosenbergii is an essential species for freshwater economic aquaculture in China, but in the larval process, their salinity requirement is high, which leads to salinity stress in the water. In order to elucidate the mechanisms regulating the response of M. rosenbergii to acute low-salinity exposure, we conducted a comprehensive study of the response of M. rosenbergii exposed to different salinities’ (0‰, 6‰, and 12‰) data for 120 h. The activities of catalase, superoxide dismutase, and glutathione peroxidase were found to be significantly inhibited in the hepatopancreas and muscle following low-salinity exposure, resulting in oxidative damage and immune deficits in M. rosenbergii. Differential gene enrichment in transcriptomics indicated that low-salinity stress induced metabolic differences and immune and inflammatory dysfunction in M. rosenbergii. The differential expressions of MIH, JHEH, and EcR genes indicated the inhibition of growth, development, and molting ability of M. rosenbergii. At the proteomic level, low salinity induced metabolic differences and affected biological and cellular regulation, as well as the immune response. Tyramine, trans-1,2-Cyclohexanediol, sorbitol, acetylcholine chloride, and chloroquine were screened by metabolomics as differential metabolic markers. In addition, combined multi-omics analysis revealed that metabolite chloroquine was highly correlated with low-salt stress.

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Effects of salinity on the accumulation of hemocyte aggregates and bacteria in the gills of Callinectes sapidus, the Atlantic blue crab, injected with Vibrio campbellii

Cited by 17 publications

References 38 publications

PtP38 May Increase the Immune Ability of Portunus trituberculatus Stimulated by LPS Imitating a Gram-Negative Bacterial Infection

PtP38 May Increase the Immune Ability of Portunus trituberculatus Stimulated by LPS Imitating a Gram-Negative Bacterial Infection

Interaction between nanomaterials and the innate immune system across evolution

Regulation and Response Mechanism of Acute Low-Salinity Stress during Larval Stages in Macrobrachium rosenbergii Based on Multi-Omics Analysis

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