Effects of Salvia Miltiorrhizae on ICAM-1, TLR4, NF-κB and Bax Proteins Expression in Multiple Organs of Rats with Severe Acute Pancreatitis or Obstructive Jaundice

Abstract

To observe the protective effects of Salvia miltiorrhizae injection on multiple organs of rats with SAP or OJ. Two hundred eighty-eight rats were used for SAP-associated experiments and OJ-associated experiments. The rats were randomly divided into sham-operated, model control and treated group. According to the different time points after operation, the SAP rats in each group were subdivided into 3, 6 and 12 h groups while the OJ rats were divided into 7, 14, 21 and 28 days groups. The mortality rates, pathological changes and ICAM-1 (only in lung), TLR4 (only in liver), Bax and NF-kappaB proteins expression in multiple organs (liver, kidney, lung, intestinal mucosa, spleen, thymus and lymph nodes) were observed, respectively. The mortality rates of treated groups decreased in both SAP and OJ experiments. Compared to model control group, the pathological changes can be seen in treated groups including; (1) the pathological changes of multiple organs in SAP and OJ experiments were improved, the pathological severity scores of kidney (at 6 h), intestinal mucosa (at 12 h), spleen (at 6 and 12 h), thymus (at 3 and 6 h) and lymph nodes (at 3 and 6 h) in SAP experiment and of liver (on 21 and 28 days), lung (21 days), intestinal mucosa (on 21 and 28 days) and spleen (on 21 and 28 days) in OJ experiment significantly declined (P < 0.05 or P < 0.01); (2) the positive rate of Bax protein in pancreas (at 3 h) and liver (on 6 and 12 h) in SAP experiment and in liver (on 28 days), kidney (on 21 days), and thymus (on 28 days) in OJ experiment were significantly lower (P < 0.05 or P < 0.01); (3) the positive rate of NF-kappaB in liver (at 12 h) and kidney (at 6 h) in SAP experiment and in liver (on 21 days), kidney (on 28 days), intestinal mucosa (on 28 days) and thymus (on 14 days) in OJ experiment significantly decreased (P < 0.05). (4) the positive rate of ICAM-1 in lungs (at 12 h) in SAP experiment was significantly less (P < 0.05). Salvia miltiorrhizae injection can protect multiple organs of SAP or OJ rats and inhibit the expression of ICAM-1, TLR4, NF-kappaB and regulate Bax proteins. Salvia miltiorrhizae may relieve the inflammation response and enhance the immunity function of SAP and OJ rats.

Effect of Salvia miltiorrhizae on Pulmonary Apoptosis of Rats with Severe Acute Pancreatitis or Obstructive Jaundice

“…The mortality rates on 7 days after operation showed no marked difference among three experimental groups; on 14 and 21 days after operation, the mortality rates in shamoperated groups were significantly lower than those in model control groups (P=0.032); on 28 days after operation, the mortality rate in sham-operated group was lower than those in both model control group (P=0.06) and treated group (P=0.032), and the difference was significant [19][20][21][22][23] (Figs. 2, 3, 4, 5, 6, 7, and 8).…”

“…Another 180 rats were utilized for OJ-associated experiments and randomly divided into sham-operated, model control and treated group (n=60), which were further randomly subdivided into 7, 14, 21 and 28 days groups (n=15) according to time duration after operation [19][20][21][22][23].…”

“…Shamoperated group was performed by the injection of saline solution instead of taurocholate, this is the only difference from the model control group. Fifteen minutes after successful operation, a single dose of Salvia miltiorrhizae injection (0.4 ml/100 g body weight) was given via femoral vein to rats in treated groups while equal volume of physiological saline solution was used in the shamoperated and model control group [19][20][21][22][23].…”

“…For rats in sham-operated groups, common bile duct was only dissociated but not ligated, and a layered suture of the abdominal wall was also performed to close the abdominal cavity. An intraperitoneal injection of Salvia miltiorrhizae injection at a dose of 0.2 ml/100 g/day was given to rats in treated groups while equal volume of physiological saline solution was used in the sham-operated and model control groups [19][20][21][22][23].…”

“…There was no marked difference in mortality rate between the time points at 3 and 6 h after operation. At 12 h after operation, only the mortality rate in model control group was significantly higher than that in sham-operated group (P=0.037), and no marked difference in mortality rate was observed among other groups (P>0.05) [19][20][21][22][23].…”