Effects of Salvia officinalis and Thymus vulgaris on oxidant-induced DNA damage and antioxidant status in HepG2 cells

Kozics, Katarína; Klusová, Veronika; Srančíková, Annamária; Mučaji, P.; Slameñová, D; Hunáková, Ľuba; Kusznierewicz, Barbara; Horváthová, Eva

doi:10.1016/j.foodchem.2013.04.089

Cited by 90 publications

(67 citation statements)

References 26 publications

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“…Kozics et al (2013) determined the viability of HepG2 cells with 24-h treatment using various concentrations of hidroalcoholic sage extract. Cells were immediately rescued from culture and MTT analysis was performed showing a IC 50 of 5.7 mg mL -1 , similar to the ExtAS in this study.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

Garcia

Menti

Lambert

et al. 2016

An. Acad. Bras. Ciênc.

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Salvia offi cinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia offi cinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH • ) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+ ), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identifi cation sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia offi cinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, infl ammation and cancer.

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Section: Discussionmentioning

confidence: 99%

Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

Garcia

Menti

Lambert

et al. 2016

An. Acad. Bras. Ciênc.

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“…[23][24][25] Moreover, our data are consistent with different studies which show that the genus Thymus exhibits anticancer potential in different cell lines, including colon cancer. 15,16,[26][27][28] Interestingly, other than thymol and carvacrol, T. vulgaris contains several other bioactive components such as luteolin, rosmarinic acid, syringic acid, apigenin glucuronide, and caffeic acid. 28 Many of these compounds have been shown to possess anticancer activities.…”

Section: Discussionmentioning

confidence: 99%

“…15,16,[26][27][28] Interestingly, other than thymol and carvacrol, T. vulgaris contains several other bioactive components such as luteolin, rosmarinic acid, syringic acid, apigenin glucuronide, and caffeic acid. 28 Many of these compounds have been shown to possess anticancer activities. [29][30][31] However, whether these molecules are responsible for the observed effects, we report in this study, remains to be investigated.…”

Section: Discussionmentioning

confidence: 99%

Thymus vulgaris (Thyme) Inhibits Proliferation, Adhesion, Migration, and Invasion of Human Colorectal Cancer Cells

Al-Menhali

Al-Rumaihi

Al-mohammed

et al. 2015

Journal of Medicinal Food

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Colorectal cancer (CRC) remains one of the most common malignancies and a leading cause of cancer-related deaths. Its prognosis remains poor for patients with several grades of this disease. This underscores the need for alternative modalities, such as herbal medicines, to treat this disease. A commonly used plant that appears to be of high medicinal value is Thymus vulgaris L. However, the effects of this plant on the malignant behavior of human CRC cells remains poorly investigated. This study was undertaken to determine the anticancer efficacy of T. vulgaris extract (TVE) in CRC cells. Our results show that TVE inhibits proliferation in a concentration- and time-dependent fashion. This decreased proliferation was concomitant with increased apoptotic cell death as evidenced by increased caspase3/7 activity. Moreover, TVE also decreased adhesion to fibronectin in a concentration-dependent manner. The migratory and invasive capacities of HCT116 cells were significantly inhibited by TVE. Taken together, these data suggest that the TVE inhibits malignant phenotype of colon cancer cells. Therefore, T. vulgaris could have an anticancer effect and that some of its bioactive compounds may prove to be effective treatment modalities for human CRC.

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“…The essential oil is the main bioactive ingredient of Salvia sclarea . In addition to being flavouring foods, Salvia sclarea essential oil can also act as antimicrobials and preservatives against food spoilage (Kozics et al 2013). …”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity and mechanisms of Salvia sclarea essential oil

et al. 2015

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BackgroundNowadays, essential oils are recognized as safe substances and can be used as antibacterial additives. Salvia sclarea is one of the most important aromatic plants cultivated world-wide as a source of essential oils. In addition to being flavoring foods, Salvia sclarea essential oil can also act as antimicrobials and preservatives against food spoilage. Understanding more about the antibacterial performance and possible mechanism of Salvia sclarea essential oil will be helpful for its application in the future. But so far few related researches have been reported.ResultsIn our study, Salvia sclarea oil showed obvious antibacterial activity against all tested bacterial strains. Minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) of seven pathogens were 0.05 and 0.1 % respectively. In addition, Salvia sclarea oil also exhibited a significant inhibitory effect on the growth of Escherichia coli (E. coli) in phosphate buffer saline (PBS) and meats. After treated with Salvia sclarea oil, Scanning Electron Microscope (SEM) images can clearly see the damage of cell membrane; the intracellular ATP concentrations of E. coli and S. aureus reduced 98.27 and 69.61 % respectively, compared to the control groups; the nuclear DNA content of E. coli and S. aureus was significantly reduced to 48.32 and 50.77 % respectively. In addition, there was massive leakage of cellular material when E. coli and S. aureus were exposed to Salvia sclarea oil.Conclusions Salvia sclarea essential oil damaged the cell membrane and changed the cell membrane permeability, leading to the release of some cytoplasm such as macromolecular substances, ATP and DNA. In general, the antimicrobial action of Salvia sclarea essential oil is not only attributable to a unique pathway, but also involves a series of events both on the cell surface and within the cytoplasm. Therefore, more experiments need to be done to fully understand the antimicrobial mechanism of Salvia sclarea essential oil.

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Effects of Salvia officinalis and Thymus vulgaris on oxidant-induced DNA damage and antioxidant status in HepG2 cells

Cited by 90 publications

References 26 publications

Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

Thymus vulgaris (Thyme) Inhibits Proliferation, Adhesion, Migration, and Invasion of Human Colorectal Cancer Cells

Antimicrobial activity and mechanisms of Salvia sclarea essential oil

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