Effects of Salvia officinalis L. on 7, 12-dimethylbenz[a]anthracene-induced breast cancer in rats and mouse mammary carcinoma cells (4T1)

Monsefi, Malihezaman; Azarbahram, Zahra; Abedian, Mehrnaz; Behrozimoghadam, Sara; Ashraf, Mohammad Javad

doi:10.1007/s00580-017-2476-5

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Most authors reported that the ITS area (ITS1; 5.8-S; ITS2 subunits) of 18S-26S nrDNA is often desired in molecular works [12]. The range of diversity in this area makes it appropriate for phylogenetic inference at the species, genus, and family levels [13,14]. Both ISSR and CDDP markers can survey a large number of loci with a small amount of plant material, making them more suitable techniques.…”

Section: Introductionmentioning

confidence: 99%

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Khal,

Tahir,

Abdul-Razaq

2023

Horticulturae

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Astragalus L. is one of the main genera of blossoming plants, and its diversity of species and forms is well known. The Astragalus L. taxa make a significant contribution to the mountainous and steppe ecosystems of the Kurdistan region of Iraq. Although the species within this genus have been the subject of several molecular studies, the evolutionary relationships among these species remain unidentified. Despite extensive research, little is known about the phylogenetic relationships among the various subgenera of Astragalus. This research is intended to investigate the molecular variation of 33 species of the genus Astragalus L. found in the Kurdistan area of Iraq. For molecular validations, three separate techniques (nuclear ribosomal DNA (rDNA), inter simple sequence repeats (ISSR), and conserved DNA-derived polymorphism (CDDP)) were used. With respect to Astragalus L. indentations, universal ITS1 and ITS4 gene sequencing was used, and the discovered sequences were subjected to BLAST searches in the NCBI database. A phylogenetic tree was generated with two main clades. Regarding detecting genetic diversity between the taxa, 24 molecular markers (14 ISSR and 10 CDDP) were used. High values of polymorphic materials and gene diversity were detected. ISSR markers had an average of 22.71 polymorphic bands per primer, while CDDP markers had the highest mean values for polymorphic information content (0.37), Shannon’s information index (0.27), expected heterozygosity (0.19), and unbiased expected heterozygosity (0.23). Cluster and principal coordinate analyses divided the Astragalus L. taxa into four main groups using the two molecular data sets. According to the results of the molecular variance analysis, the highest variation was detected within sections, with values of 92.01 and 89.48% for ISSR and CDDP markers, respectively. These outcomes suggest the effectiveness of molecular markers and the ITS region in determining and identifying genetic correlations between Astragalus species.

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Section: Introductionmentioning

confidence: 99%

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Khal,

Tahir,

Abdul-Razaq

2023

Horticulturae

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“…Salvia officinalis is one of the most important Salvia species which its anti-proliferative effects have been reported on different cancer cells. [8][9][10] In an investigation on the cytotoxic effects of eleven local Salvia species on three HL60, K562 and MCF-7 cell lines, the anticancer effects of S. eremophila, S. santolinifolia, S. aegyptiaca, S. aethiopis, S. hypoleuca and S. limbata was found to be significant. 11 Noori et al have shown that Sclareol which has been isolated from Salvia sclarea induced a decrease in tumor size of breast cancer mouse model.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Effects of Salvia chorassanica Bunge and Shoot Extracts on Gastric Cancer Cells: Evidence of Different Behavior on Various Tumor Grades

et al. 2020

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Background: Different Salvia species have demonstrated anti-proliferative effects on various cancer cells. Owing to the poor literature on the anti-proliferative effects of Salvia species on gastric cancer cells, present study was conducted to determine the anticancer effects of a local Iranian Salvia, Salvia chorassanica, on two different gastric cell lines. Methods: Root, stem and leaf extract of Salvia chorassanica were prepared through maceration method and were then used to treat the AGS and MKN-45 cell lines in different concentrations. MTT assay was employed to determine the toxicity of all the types of extracts on the two studied cell lines. The expression of Bax, Bcl-2, Caspase3, MMP2 and MMP9 genes were determined through reverse transcription Real time PCR (RT-PCR). Results: Bunge and shoot extracts demonstrated toxicity in both cell lines which were more considerable in AGS cells treated with root extract. In contrary to AGS cells, Caspase3 gene was up-regulated in all types of treatment while the MMP2 and MMP9 genes were down-regulated (p-value<0.001). Except of the MKN-45 cells treated with leaf extract, Bax/Bcl-2 expression ratio was decreased in the treatment with all types of Salvia chorassanica extracts (p-value<0.001). Conclusion: Remarkable low IC50 concentration of root extract in MKN-45 cell line is indicating the significant cytotoxicity of Salvia chorassanica against gastric cancer cells. Moreover, gene expression analysis in MKN-45 needs further confirmation on the potential anti-metastatic roles of leaf and root extracts in higher grades of gastric cancer.

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Effects of Salvia officinalis L. on 7, 12-dimethylbenz[a]anthracene-induced breast cancer in rats and mouse mammary carcinoma cells (4T1)

Cited by 2 publications

References 17 publications

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Molecular Variation in Some Taxa of Genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region

Investigating the Effects of Salvia chorassanica Bunge and Shoot Extracts on Gastric Cancer Cells: Evidence of Different Behavior on Various Tumor Grades

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