Effects of sample size on neutral detergent fiber digestibility of triticale forages using the Ankom DaisyII Incubator system

Abstract

Accurate and precise determinations of fiber digestibility are essential for proper diet formulation for dairy cows. Our objectives were 3-fold: (1) regress in vitro neutral detergent fiber digestibility (NDFD) values from 48 triticale forages determined at multiple endpoints ranging from 12 to 240 h with Ankom Daisy II Incubator system (Ankom Technology Corp., Macedon, NY) methods using 0.25-or 0.50-g sample sizes on concentrations of fiber-related analytes or growth stage; (2) directly compare NDFD values determined with 0.25-or 0.50-g sample sizes by Ankom methods after 12-, 24-, 30-, 48-, 144-, or 240-h incubations; and (3) compare NDFD values determined by Ankom methods after 30 and 48 h of incubation with those determined by traditional sealed-tube procedures obtained from a commercial laboratory. Generally, plant growth stage, which was quantified with a linear model suitable for serving as an independent regression variable, proved to be a better predictor variable for NDFD than neutral detergent fiber or acid detergent lignin. For direct comparisons of 0.25-and 0.50-g sample sizes using Ankom methods, the regression relationship for a 30-h incubation was explained by a linear model [Y = 1.206x -1.1; coefficient of determination (R 2 ) = 0.933], in which the slope differed from unity, but the intercept did not differ from 0. After a 48-h incubation, a linear model (Y = 1.014x + 7.1; R 2 = 0.964) indicated that the slope did not differ from unity, but the intercept was >0. A linear regression (Y = 1.040x -1.8; R 2 = 0.861) of the 30-h incubation results obtained by Ankom methods using the 0.25-g sample size on those from the commercial laboratory indicated the slope and intercept did not differ from unity or 0, respectively. A similar relationship was obtained from the 48-h incubation (Y = 1.021x -3.4; R 2 = 0.866). Relationships were poorer when the 0.50-g sample size was used by Ankom methods, particularly for the 30-h incubation, where the slope (0.824) was less than unity. Generally, NDFD values were greater for the 0.25-g sample size by Ankom methods, especially with 24-, 30-, and 48-h incubation times, and agreement with traditional sealed-tube methods was improved with the smaller sample size. Synchronization of results between Ankom and traditional methods needs to be further verified across a wider range of forages and harvest/preservation methods before definitive recommendations can be made.

CARACTERIZACIÓN, DIGESTIBILIDAD Y EFECTO PREBIOTICO DEL EXTRACTO DEL FRUTO DE Stenocereus queretaroensis F.A.C. WEBER BUXBAUM

“…Digestion in the large intestine was investigated by in vitro fermentation with the Daisy II Method as described by Coblentz et al (2019) with some modifications. Before starting the test, solutions A, B, and fecal inoculum were prepared.…”

“…As a result, high digestibility values are obtained. The large intestine digestibility was adapted for humans by combining the Daisy II and Chen methods (Chen et al, 2018;Coblentz et al, 2019). Until now, only the Daisy II method has been described for ruminant digestibility.…”

Effect of Irrigation Volume on Biomass and Nutritional Value of Zea mays L. as Green Hydroponic Forage

“…It is important to note that the low digestibility percentages of DM obtained by Herrera et al (2007) could be due to the experimental conditions in which the study was conducted and to the characteristics of the plant material used. The digestibility values for NDF and ADF were above those obtained with other conventional forages (Naranjo and Cuartas, 2011;Coblentz et al, 2019). This could be because of the physical characteristics of the GHMF, since it is a seedling composed mainly of young leaves with more digestible cell walls.…”

Dry matter yield and nutritive value of early‐ or late‐maturing spring wheat, spring barley, and oat cultivars planted in late summer

“…Water‐soluble carbohydrates (WSC) were determined following extraction of 0.25‐g samples of dried, ground forage in 5 oz of deionized water for 2 h. The resulting slurries were gravity filtered through Whatman #1 filter paper (GE Healthcare UK Limited, Little Chalfont, UK) before concentrations of WSC were determined via the phenol–sulfuric acid reaction (Dubois, Gilles, Hamilton, Rebers, & Smith, 1956) followed by colorimetric analysis with a double‐beam spectrophotometer with dextrose used to formulate appropriate standards. In addition, in vitro DM digestibility and NDF fiber digestibility (NDFD) incubations were conducted for 30 and 48 h via procedures similar to those described by Coblentz, Akins, Ogden, Bauman, and Stammer (2019) for a Daisy II Incubator (ANKOM Technology Corp.). For these analyses, 0.5‐g samples were sealed in each F‐57 fiber bag and rumen fluid was obtained from two nonlactating cows consuming a forage‐based diet.…”