Effects of saturated palmitic acid and omega-3 polyunsaturated fatty acids on Sertoli cell apoptosis

Hu, Xuechun; Ge, Xie; Liang, Wei; Shao, Yina; Jing, Jun; Wang, Cen-Cen; Zeng, Rong; Yao, Bing

doi:10.1080/19396368.2018.1471554

Cited by 50 publications

(39 citation statements)

References 38 publications

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“…In a recent in vitro study, n-3 PUFA have been classified as protective for Sertoli cells [41]. EPA was mainly localised in the interstitial tissue of the control rabbits, but it was more abundant in the testes from the n-3 enriched groups, both in the interstitial tissue and germ cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Castellini

Mattioli

Signorini

et al. 2019

Oxidative Medicine and Cellular Longevity

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In the last two decades, the human sperm count linearly decreased in Western countries. Health problems, lifestyle, pollutants, and dietary behaviours are considered as the main risk factors, and the unbalance of dietary n‐6/n‐3 fatty acids is one of the most relevant. The aim of the present research is to study the effect of different dietary sources of n‐3 polyunsaturated fatty acids (PUFA) on reproductive traits using rabbit buck as the animal model. Fifteen rabbit bucks were assigned to three experimental groups: the control group, the FLAX group fed 10% extruded flaxseed, and the FISH group fed 3.5% fish oil for 110 days (50-day adaptation and 60-day experimental periods). Semen samples were collected weekly, whereas blood was collected every two weeks for the analytical determination of semen traits, oxidative status, fatty acid profiles, isoprostanes, neuroprostanes, and the immunocytochemistry of docosahexaenoic acid (DHA) and eicosapentaenoic (EPA) acid. At the end of the trial, the rabbits were killed and the testes were removed and stored for the analysis of fatty acid profile and immunocytochemistry. Results showed that dietary administration of n‐3 PUFA improved the track speed of the sperm and increased the n‐3 long-chain PUFA mainly confined in the sperm tail. Seminal plasma increased the thiobarbituric reactive substances (TBARs) by three times in the groups fed supplemental n‐3, whereas the F2-isoprotanes (F2-IsoPs) and F4-neuroprostanes (F4-NeuroPs) were lower and higher, respectively, in both supplemented groups than in the control. The testes and sperm showed a higher DHA and EPA distribution in rabbits from the n‐3 supplemented groups compared with the control. In conclusion, supplemental dietary n‐3 PUFA improved sperm motion traits and resulted in an enrichment of membrane fatty acid in the sperm and testes of the rabbits. However, such an increased amount of PUFA negatively affected the sperm oxidative status, which was mainly correlated with the generation of F4-NeuroPs with respect to F2-IsoPs. Accordingly, the latter cannot be considered a good marker of oxidation when diets rich in n‐3 PUFA are provided.

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Section: Discussionmentioning

confidence: 99%

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Castellini

Mattioli

Signorini

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Omega-6 fatty acids have possessed diverse bioactivities and are associated with many beneficial effects on human health including cancer development [27]. Recently [28], omega-3 (ω-3) polyunsaturated fatty acids were reported to protect Sertoli cells apoptosis at the physiologically relevant levels [28]. In our present work, the glutamic acid concentration in all genotypes is the highest values in all identified amino acids.…”

Section: Discussionmentioning

confidence: 48%

Comparative Phytochemical and Biological Investigation of Five Glycine Max (L.) Merrill Genotypes

Abd‐Alla

Taie

Abd-Elmotaleb

2019

Asian J Pharm Clin Res

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Objective: Soybean (Glycine max L. Merrill) is the world’s most important consumed seed legume. The objectives of the present study were to determine the variability in phytochemical composition and biological activities between five genotypes of G. max. Methods: Lipoidal matters were determined using glucose (GLC). Amino acids were detected by the amino acid analyzer. The phytoconstituents present within each ethanol extract was investigated by gas chromatography-mass spectrometry. The amount of total phenolics, flavonoids, and tannins was analyzed using a spectrophotometric technique, based on Folin–Ciocalteu reagent, aluminum chloride colorimetric assay, and the modified vanillin hydrochloric acid method, respectively. Quercetin, catechin, and gallic acid were used as standard compounds, respectively. Isoflavones content were detected by high-performance liquid chromatography (HPLC)/photodiode array (PDA). The radical scavenging and antioxidant capacity of the genotypes using different in vitro analytical assays such as 2,2-diphenyl,1-picryl hydrazyl, 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, reducing power, metal chelating, and ferric reducing anti-oxidant power. Butyl hydroxyl toluene and trolox were used as the reference antioxidant radical scavenger compounds. Antitumor activity was evaluated by detecting the viability of Ehrlich ascites carcinoma cells on four different concentrations (1–5 mg/mL). Results: GLC analysis showed the high value of total unsaturated fatty acids and 16 amino acids including glutamic acid with the highest concentration. The variation between genotypes according to their chemical composition of the aldehydes, esters, ketones, alcoholics, and carboxylic content were reported. HPLC/PDA referred to the presence of daidzein, genistein, and in all genotypes. Conclusion: The results confirm the higher value of phytoconstituents of the genotype Giza 35 and Giza 21 as well as their better bioactivity.

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“…Subsequently, the slides were stained with hematoxylene-eosin (H&E). The steps required for staining of the testicular sections with ZO-1 (ab96587, Abcam, USA) and MVH (ab13840, Abcam, USA) antibodies were the same as described previously [ 25 ]. Briefly, the sections were hydrated with gradient alcohol followed by dewaxing with xylene, blocked with 5% bovine serum albumin (BSA, Sigma) for 1 h at room temperature and finally incubated with ZO-1 and MVH antibodies at 4 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

Spermatogenesis improved by suppressing the high level of endogenous gonadotropins in idiopathic non-obstructive azoospermia: a case control pilot study

Ding

Hong

et al. 2018

Reprod Biol Endocrinol

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BackgroundElevated plasma gonadotropins were associated with desensitization of Sertoli and Leydig cells in the male testis. Testis spermatogenesis ability would be improved via inhibiting high endogenous gonadotropin in patients with severe oligozoospermia. Whether it would be beneficial for non-obstructive azoospermia (NOA) patients was still unclear.MethodsGoserelin, a gonadotropin releasing hormone agonist (GnRHα) was used to suppress endogenous gonadotropin levels (gonadotropin reset) in the NOA patients, improving the sensitization of the Sertoli and Leydig cells. Then human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG) were injected to stimulate them to ameliorate the ability of testicular spermatogenesis. The main outcome measure was the existence of spermatozoa in the semen or by testicular sperm extraction (TESE). Elevation of inhibin B and/or ameliorative expression pattern of ZO-1 was the secondary objective.ResultsA total of 35 NOA men who failed to retrieve sperm via TESE were enrolled. Among these, 10 patients without treatment were selected as control group and secondary TESE was performed 6 months later. Of the 25 treated men, inhibin B was elevated in 11 patients in the first 4 weeks (Response group), while only 5 patients had constant increase in the following 20 weeks (Response group 2). Of the 5 men, 2 men acquired sperm (Response group 2B), while 3 failed (Response group 2A). Immunofluorescence of mouse vasa homologue (MVH) and ZO-1 showed that both positive MVH signals and ZO-1 expression were significantly increased in the Response group 2, but only Response group 2B showed ameliorative ZO-1 distribution.ConclusionsGonadotropin reset, a new therapeutic protocol with GnRHα, was able to improve the ability of testicular spermatogenesis in the NOA patients through restoring the sensitivity of Sertoli and Leydig cells, which were reflected by elevated inhibin B and ameliorative ZO-1 expression and distribution.Trial registrationClinicalTrials.gov identifier: NCT02544191.Electronic supplementary materialThe online version of this article (10.1186/s12958-018-0401-7) contains supplementary material, which is available to authorized users.

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Effects of saturated palmitic acid and omega-3 polyunsaturated fatty acids on Sertoli cell apoptosis

Cited by 50 publications

References 38 publications

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Comparative Phytochemical and Biological Investigation of Five Glycine Max (L.) Merrill Genotypes

Spermatogenesis improved by suppressing the high level of endogenous gonadotropins in idiopathic non-obstructive azoospermia: a case control pilot study

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