Effects of<scp><i>Oxalis barrelieri</i></scp>L. (Oxalidaceae) aqueous extract on diarrhea induced by<i>Shigella dysenteriae</i>type 1 in rats

Tagne, Michel Archange Fokam; Noubissi, Paul Aimé; Fankem, Gaétan Olivier; Kamgang, René

doi:10.1002/hsr2.20

Cited by 14 publications

(8 citation statements)

References 35 publications

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“…In vitro , Crinum jagus extract showed concentration-dependent antibacterial activities. The MIC/MBC ratio was lower than 4 and could thus indicate a bactericidal activity [ 26 ]. This activity was further confirmed by the reduced bacterial load observed with in vivo studies.…”

Section: Discussionmentioning

confidence: 99%

Effects of Crinum jagus Water/Ethanol Extract on Shigella flexneri-Induced Diarrhea in Rats

Noubissi

Tagne

Fankem

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Diarrheal disease, characterized by the release of more than three loose or liquid stools per day, remains one of the leading causes of morbidity and mortality in children below 5 years of age in developing countries. Many drugs used in diarrhea management face contraindication and, with regard to infectious diarrhea, resistance of some bacterial strains; this therefore increases the need of new alternative and more effective drugs. This study aimed to evaluate anti-Shigella flexneri activities of Crinum jagus water/ethanol extract. In vitro activities were assayed by disc diffusion and agar dilution methods and in vivo section on Shigella flexneri-induced diarrhea in rats. This was done by oral administration of 9 X 108 CFU of Shigella flexneri to rats that were treated twice daily with Crinum jagus water/ethanol extract for seven consecutive days. Ciprofloxacin was used as positive control. Daily Shigella flexneri load was evaluated. After one treatment week, animals were then sacrificed and interleukins (IL-2 and INF-γ), immunoglobulins (IgA and IgM), motilin, vasoactive intestinal peptide, and ions (sodium, potassium, calcium, and chloride) levels were determined. Also, blood cell count was realized. Crinum jagus water/ethanol extract dose-dependently inhibited Shigella flexneri growth with inhibition diameter of 18.90 and 25.36 mm, respectively, at 0.39 and 200 mg/mL. Minimum inhibitory concentration (MIC) was 0.10 mg/mL and minimum bactericidal concentration (MBC) was 0.30 mg/mL with MBC/MIC ratio of 3.0. In Shigella flexneri-induced diarrheic rats, Crinum jagus reduced (p<0.01) diarrheal stools emission and Shigella load and lowered IL-2, INF-γ, IgA, IgM, and motilin blood levels, whereas it increased (p<0.01) vasoactive intestinal peptide, sodium, potassium, calcium, and chloride blood levels. In diarrheal rats, Crinum jagus restored the decreasing white blood cells and haemoglobin and restored the damaged colon epithelium, where it reduced the density of mucus-filled goblet cells. These results confirm the use of Crinum jagus in ethnomedicine in diarrhea treatment.

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Section: Discussionmentioning

confidence: 99%

Effects of Crinum jagus Water/Ethanol Extract on Shigella flexneri-Induced Diarrhea in Rats

Noubissi

Tagne

Fankem

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Oxalis barrelieri is a medicinal plant used to treat certain pathologies such as diabetes [13] and diarrhea [7,8]. In this study, the objective was to evaluate the toxic effects of this plant on biological systems, particularly in the liver and kidneys.…”

Section: Discussionmentioning

confidence: 99%

“…Enoch et al [6] reported that administration of 500 mg/kg and 1000 mg/kg aqueous and ethanolic extracts of O. barrelieri on Sprague-dawley rats produced significant reductions of glycemia in both non-diabetic and diabetic rats. A decoction of the entire plant is used for the treatment of diarrhea [7,8]. O. barrelieri is rich in phenols, flavonoïds, tannins, alkaloids and saponins [9].…”

Section: Introductionmentioning

confidence: 99%

Acute and Subacute Toxicity of Oxalis barrelieri (Oxalidaceae) Aqueous Aerial Parts Extract

Mezui

Amang

Mbomo

et al. 2019

JABB

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Aims: The present study was carried out to investigate the toxic effects of the Oxalis barrelieri aqueous aerial parts extract. Place and Duration of Study: Department of Biological Sciences (Animal Physiology Laboratory), Higher Teachers’ Training College, University of Yaoundé I. Between April 2017 and June 2018. Materials and Methods: Acute toxicity using a single dose of 2000 mg/kg was administered to mice and effects were observed for 14 days. In sub-acute toxicity, the experimental rats (males and females) received aqueous extract of Oxalis barrelieri at doses of 200 mg/kg, 400 mg/kg and 800 mg/kg daily for 28 days while the control and satellite control groups received distilled water and satellite test group received extract at the dose of 800 mg/kg. The physical parameters were evaluated throughout the treatment, while the haematological, biochemical and histological parameters were evaluated at the end of the treatment. Results: In acute toxicity, the results obtained show no death and no significant variation (p>0.05) in behavioral and morphological parameters. In sub-acute toxicity assay, few modifications were observed in haematological and biochemical parameters. At the higher dose of extract (800 mg/kg),the rate of red blood cells decreased significantly (p<0.05) two weeks after treatment in male rats , there were a significant increase (P˂0.001) in ASAT activity in male and female rats two weeks after extract administration, and a reversible significant increase (P˂0.05) in triglyceride level in male rats only. Histopathology showed a reversible slight dose dependent structural alteration of the kidney and reversible vascular congestion in liver. Conclusion: The aqueous aerial parts extract of Oxalis barrelieri could possess moderate toxicity at high doses and adequate caution should be exercised in its use in ethnomedicine.

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“…They can be of infectious origins (bacterial, viral, parasitic or fungal), inflammatory, tumoral or medicinal (antibiotics). Infectious diarrhea is caused by bacteria such as Salmonella, Shigella [2] and Entomopathogenic colibacilli [3]; by viruses such as parvoviruses, rotaviruses, reoviruses [4], or by parasites such as amoebae and helminths [5]. These infections increase in case of lack of safe water for various household chores and for consumption.…”

Section: Introductionmentioning

confidence: 99%

Effects of hydroethanolic extract of Cameroonian propolis (Promax-c) on castor oil-induced diarrhea in mice

Tagne¹,

Noubissi²,

Tchoblaouna³

et al. 2020

World J. Adv. Res. Rev.

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The aim of our work was to evaluate the effect of hydroethanolic extract of Cameroonian propolis (Promax-c) on castor oil-induced diarrhea in mice. Diarrhea was induced in mice by oral administration 0.5 mL of castor oil in all mice. To determine the effective doses, each mouse received, 30 minutes after the administration of castor oil, one of the single oral doses of Promax-c: 0, 37.5, 75, 150, and 300 mg/kg bw. The mass and frequency of stool were measured and recorded per hour for five hours. The effect of Promax-c on the intestinal motility was evaluated by measuring the distance traveled by the charcoal meal in thirty minutes. The effects of Promax-c on intestinal secretion were evaluated by measuring the volume of the intestinal content and by dosing the electrolytes (Na + , K + , and Cl -) in the intestinal content by the colorimetric method. Promax-c produced significant (P <0.01) decreases, respectively, 29.16%, 62.50%, 62.50%, and 79.17% in the severity of diarrhea. Promax-c at 75 and 150 mg/kg bw exhibited significant (P <0.01) reduction on intestinal transit (12.30% and 24.86%, respectively) as compared to normal control and showed a significant (P <0.01) decrease of castor oil-induced enteropooling (48.64% and 56.75%, respectively) as compared to diarrheal control. This extract significantly (P <0.01) reduced the secretion of electrolytes (Na + , K + and Cl -) and kept the stool osmotic gap at a normal value. The results we obtained allow us to affirm that the ethanolic extract of propolis (Promax-c) would have an antidiarrheal activity.

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Effects ofOxalis barrelieriL. (Oxalidaceae) aqueous extract on diarrhea induced byShigella dysenteriaetype 1 in rats

Cited by 14 publications

References 35 publications

Effects of Crinum jagus Water/Ethanol Extract on Shigella flexneri-Induced Diarrhea in Rats

Effects of Crinum jagus Water/Ethanol Extract on Shigella flexneri-Induced Diarrhea in Rats

Acute and Subacute Toxicity of Oxalis barrelieri (Oxalidaceae) Aqueous Aerial Parts Extract

Effects of hydroethanolic extract of Cameroonian propolis (Promax-c) on castor oil-induced diarrhea in mice

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