Effects of
            <scp>l</scp>
            -Serine on Macrolide Resistance in Streptococcus suis

Wu, Tong; Wang, Xiaozhen; Dong, Yue; Xing, Chaoping; Chen, Xueying; Li, Lü; Dong, Chunliu; Li, Yanhua

doi:10.1128/spectrum.00689-22

Cited by 7 publications

(5 citation statements)

References 52 publications

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“…In veterinary medicine, macrolides and lincosamides can be used as feed and injection preparations for treating swine respiratory tract infections ( 14 , 36 ). In addition, tetracycline, florfenicol, and penicillins are frequently applied separately or in combination for treating S. suis infections ( 37 – 39 ). Notably, we found linezolid and vancomycin resistance (LVR), and these are last-resort antimicrobial agents for the treatment of multidrug-resistant Gram-positive bacterial infections, which poses a severe threat to public health.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Patterns and Molecular Characterization of Streptococcus suis Isolates from Swine and Humans in China

Wang

Chu

et al. 2023

Microbiol Spectr

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Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Patterns and Molecular Characterization of Streptococcus suis Isolates from Swine and Humans in China

Wang

Chu

et al. 2023

Microbiol Spectr

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“…The formation of disulfide bridges was also dispensable in certain antimicrobial peptides and the reduction in disulfide bonds increased the antimicrobial activity [34][35][36][37][38]. The addition of serine in combination with some antibacterial drugs enhanced the antimicrobial activity [39][40][41]. We also investigated the role of tryptophans in the studied peptide, as they may be necessary for the interaction of AMPs with bacterial membranes [42][43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Antibacterial Activity of Substituted Derivatives of NCR169C Peptide

Howan

Jenei

Szolomájer

et al. 2023

IJMS

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Medicago truncatula in symbiosis with its rhizobial bacterium partner produces more than 700 nodule-specific cysteine-rich (NCR) peptides with diverse physicochemical properties. Most of the cationic NCR peptides have antimicrobial activity and the potential to tackle antimicrobial resistance with their novel modes of action. This work focuses on the antibacterial activity of the NCR169 peptide derivatives as we previously demonstrated that the C-terminal sequence of NCR169 (NCR169C17–38) has antifungal activity, affecting the viability, morphology, and biofilm formation of various Candida species. Here, we show that NCR169C17–38 and its various substituted derivatives are also able to kill ESKAPE pathogens such as Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli. The replacement of the two cysteines with serines enhanced the antimicrobial activity against most of the tested bacteria, indicating that the formation of a disulfide bridge is not required. As tryptophan can play role in the interaction with bacterial membranes and thus in antibacterial activity, we replaced the tryptophans in the NCR169C17–38C12,17/S sequence with various modified tryptophans, namely 5-methyl tryptophan, 5-fluoro tryptophan, 6-fluoro tryptophan, 7-aza tryptophan, and 5-methoxy tryptophan, in the synthesis of NCR169C17–38C12,17/S analogs. The results demonstrate that the presence of modified fluorotryptophans can significantly enhance the antimicrobial activity without notable hemolytic effect, and this finding could be beneficial for the further development of new AMPs from the members of the NCR peptide family.

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“…In view of this, increasing ROS production by regulating metabolic networks may enhance antibiotic activity [106]. Recently, Wu et al [110] found that L-serine is an ROS enhancer that can produce synergistic killing effects against macrolide-resistant Streptococcus suis in vitro and in vivo when combined with macrolide antibiotics. Further studies found that L-serine inhibited the production of intracellular H 2 S, reduced the production of Fe-S clusters, and restored the intracellular Fenton reaction, which, eventually, caused an increase in the level of bacterial endogenous reactive oxygen species, leading to intracellular DNA damage and bacterial death.…”

Section: Activation Of the Bacterial Metabolic Statementioning

confidence: 99%

The Combination of Antibiotic and Non-Antibiotic Compounds Improves Antibiotic Efficacy against Multidrug-Resistant Bacteria

Xiao,

Li,

Sun

2023

IJMS

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Bacterial antibiotic resistance, especially the emergence of multidrug-resistant (MDR) strains, urgently requires the development of effective treatment strategies. It is always of interest to delve into the mechanisms of resistance to current antibiotics and target them to promote the efficacy of existing antibiotics. In recent years, non-antibiotic compounds have played an important auxiliary role in improving the efficacy of antibiotics and promoting the treatment of drug-resistant bacteria. The combination of non-antibiotic compounds with antibiotics is considered a promising strategy against MDR bacteria. In this review, we first briefly summarize the main resistance mechanisms of current antibiotics. In addition, we propose several strategies to enhance antibiotic action based on resistance mechanisms. Then, the research progress of non-antibiotic compounds that can promote antibiotic-resistant bacteria through different mechanisms in recent years is also summarized. Finally, the development prospects and challenges of these non-antibiotic compounds in combination with antibiotics are discussed.

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Effects of l -Serine on Macrolide Resistance in Streptococcus suis

Abstract: The emergence of antimicrobial resistance is a global challenge. However, new drug development efforts consume considerable resources and time, and alleviating the pressure on existing drugs is the focus of our work.

Cited by 7 publications

References 52 publications

Antibiotic Resistance Patterns and Molecular Characterization of Streptococcus suis Isolates from Swine and Humans in China

Antibiotic Resistance Patterns and Molecular Characterization of Streptococcus suis Isolates from Swine and Humans in China

Enhanced Antibacterial Activity of Substituted Derivatives of NCR169C Peptide

The Combination of Antibiotic and Non-Antibiotic Compounds Improves Antibiotic Efficacy against Multidrug-Resistant Bacteria

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