Effects of scriptaid on the histone acetylation of buffalo oocytes and their ability to support the development of somatic cell nuclear transfer embryos

Histone Lactylation Is Involved in Mouse Oocyte Maturation and Embryo Development

“…Moreover, our examination of specific lysine residues revealed H3K9la, H3K14la, H4K8la, and H4K12la fluorescence was strongest at GV but diminished at GVBD and MII, while H4K5la peaked at GVBD, suggesting lactylation at different sites may confer distinct functions during maturation. Analogous to our findings on lactylation dynamics, studies report changes in histone acetylation patterns like H3K18 acetylation increasing from GV to GVBD, peaking at GVBD, deacetylating at MI, and reacetylating at MII in buffalo oocytes [23]. Similarly, methylation patterns like H3K9me2 (a heterochromatin marker) tend to decrease in aged mouse GV oocytes compared to young ones [24], which could impact chromatin states and transcription.…”

Histone deacetylase inhibitor during in vitro maturation decreases developmental capacity of bovine oocytes

“…It is interesting to note that treatments with scriptaid at any time (PIVM or IVM) showed no difference in the expression of any evaluated gene, suggesting that the acetylation may remain unchanged until the stage of MII owing to the use of scriptaid. In agreement with this, similar results were reported by Sun et al [34], who used the same concentration of scriptaid in IVM of buffalo oocytes and found that it induced an increase in the expression of acetylation related genes in MII, including HAT1. Although we were expecting to see a change on the expression of genes besides HAT1, some studies have shown that treatments with HDACis may influence a small percentage of genes [58,59].…”

“…Selected COCs were washed and transferred in number of 25 to 30 to a 150 μl drop of either PIVM or maturation medium covered with mineral oil, and then cultured for 6 h at 38.5˚C in 5% CO 2 . PIVM medium consisted of TCM-199 with Earle's salts (Gibco1, Invitrogen, Carlsbad, CA, USA) supplement with 0.075 mg/ml of amikacin, 0.2% free fatty acid albumin (BSA-FAF), 0.68 mM of L-glutamine, 1 mM of sodium pyruvate, 0.1 μM of cysteamine, 10 −4 IU/ml recombinant follicle stimulating hormone [rFSH (Gonal-F1, Merck Serono, Rockland, MA, USA), and a meiotic inhibitor supplemented or not supplemented with scriptaid at the concentration of 500 nM [34]. The meiotic inhibitor used was NPPC at the concentration of 100 μM [14].…”

“…Among the HDACi available we chose to use scriptaid because it has been used with success in the production of clone embryo [34,[43][44][45][46], and specially due to its low toxicity [47]. Because there is no information regarding the use of scriptaid during maturation of bovine oocytes, we did a pre-experiment aiming to define how long scriptaid in culture should be kept.…”

“…These inhibitors have been used mainly in embryo culture media to improve the development of clone embryo produced by somatic cell nuclear transfer (SCNT) [29][30][31]. Few studies reported the use of HDACi during maturation [32,33] however, when scriptaid was used during IVM of buffalo oocytes, the results showed an increased expression of genes related to acetylation, and improvement in the production of SCNT embryos [34].…”