Effects of seawater temperature and acute Vibrio sp. challenge on the haemolymph immune and metabolic responses of adult mussels (Perna canaliculus)

Ericson, Jessica A.; Venter, Leonie; Welford, Mena R.V.; Kumanan, K.; Ragg, Norman L.C.

doi:10.1016/j.fsi.2022.08.015

Cited by 13 publications

(15 citation statements)

References 81 publications

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“…The C16 and C18 fatty acids detected in the coinfected group of mussels in the current study can thus be attributed to a bacterial response, in addition to the mussel response. Pathogenic bacteria are known to utilize compounds other than glucose as carbon sources for growth, such as fatty acids (Ericson et al, 2022). With the reduction in the levels of fatty acids (arachidonic acid, gondoic acid, hexadecanoic acid, margaric acid, octadecanal, and pentadecanoic acid) in the current study, it can be suggested that Vibrio sp.…”

Section: Coinfection Responsementioning

confidence: 66%

“…Pathogenic bacteria are known to utilize compounds other than glucose as carbon sources for growth, such as fatty acids (Ericson et al, 2022). With the reduction in the levels of fatty acids (arachidonic acid, gondoic acid, hexadecanoic acid, margaric acid, octadecanal, and pentadecanoic acid) in the current study, it can be suggested that Vibrio sp.…”

Section: Discussionmentioning

confidence: 99%

“…A pipette tip was used to maintain the shell opening, and a prechilled needle (25 gauge) attached to a 3 mL syringe was inserted through the opening and into the posterior adductor muscle. Approximately 0.5-1.0 mL of haemolymph was sampled, of which 50 μL was transferred into a microcentrifuge tube for thiosulfate-citrate-bile salts-sugar (TCBS) agar plating, and 400 μL was transferred into a cryo vial and then flash frozen in liquid nitrogen for later metabolomics analyses (Ericson et al, 2022).…”

Section: Mussel Bacterial Challenge and Samplingmentioning

confidence: 99%

“…Considering that the metabolic response is taking place in both the host (in response to the pathogen) and the pathogen (as it adapts and proliferates in the host environment), the metabolite results should also be considered a consequence of the bacteria and not only the mussel, as previously demonstrated in Vibrio sp. infected P. canaliculus (Ericson et al, 2022). Metabolite profiling of V. parahaemolyticus fatty acids reported hexadecenoic (16:1), hexadecanoic (16:0), and octadecenoic (18:1) acids as the major fatty acids of Vibrio strains (Jia et al, 2014).…”

Section: Coinfection Responsementioning

confidence: 99%

“…Changes in metabolic pathways can inform how pathogen infections induce stress‐related mechanisms and affect molecular regulatory processes (Li et al, 2020). To date, experiments studying Vibrio spp.‐related infections in P. canaliculus using metabolomics have only focused on singular pathogenic infections (Ericson et al, 2022; Nguyen & Alfaro, 2020; Nguyen, Alfaro, Young, et al, 2018), highlighting that more research is needed to understand the responses of mussels during coinfections.…”

Section: Introductionmentioning

confidence: 99%

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Investigating the effect of bacterial coinfections on juvenile and adult green‐lipped mussels (Perna canaliculus)

Azizan,

Carter,

Venter

et al. 2023

J World Aquaculture Soc

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The New Zealand's Greenshell™ mussel (Perna canaliculus) aquaculture industry is being affected by summer mortality events associated with increasing seawater temperatures and pathogens. In this study, challenge experiments were conducted to investigate, for the first time, the effects of pathogen coinfection on the survivability and haemolymph immune responses of juvenile and adult mussels. Animals were injected with marine broth (control), Vibrio mediterranei, Photobacterium swingsii, or a mixture of V. mediterranei and P. swingsii. Then, mussel survival was monitored for 72 h, and haemolymph was sampled for bacterial quantification and metabolomics analyses at 24‐ and 48‐h post challenge. Coinfected adults and juveniles showed 100% mortality. Bacterial colony counts in haemolymph decreased as infection time continued, especially in juveniles. The haemolymph metabolome of mussels exposed to single bacterial species and coinfection showed response changes largely within energy metabolism. Mussels infected with V. mediterranei exhibited increased metabolites linked to the glutathione pathway, branched‐chain amino acids, and others over time, supporting structural functions. Conversely, mussels infected with P. swingsii showed no metabolic differences over time. The coinfection group exhibited large decreases in important metabolites, such as fatty acids as an alternative energy source and amino acids to support immune functions and protein synthesis.

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Section: Coinfection Responsementioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Mussel Bacterial Challenge and Samplingmentioning

confidence: 99%

Section: Coinfection Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Investigating the effect of bacterial coinfections on juvenile and adult green‐lipped mussels (Perna canaliculus)

Azizan,

Carter,

Venter

et al. 2023

J World Aquaculture Soc

Self Cite

View full text Add to dashboard Cite

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Biomarkers of mussel exposure to Vibrionaceae: A review

Azizan,

Venter,

Alfaro

2024

Aquacult Int

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For more than a century, bacterial infections caused by Vibrio spp. have affected various species of mussels around the world, with limited insights into the responses implemented by mussels against these infections. A combination of chemical analyses and carefully selected biological endpoints from haemolymph and tissues can be used to identify the welfare status of mussels and potentially protect aquatic ecosystems from catastrophic health threats. Recent developments in biomarker identification tools, such as omics and bioinformatics, have been successfully applied to evaluate the effect of environmental pollutants and other chemicals on mussels. However, the application of biomarkers to assess mussel health is limited. This review describes the available scientific literature on biomarker research for Vibrio-mussel interactions, and those aspects related to mussel health and disease assessment, grouped as biomarkers of exposure, effects, and susceptibility. From the review, it is clear that when integrated biomarkers are used, they can provide a deeper understanding of the relative health and potential susceptibility of mussels for better management practices. Furthermore, health biomarker data can be used to build resilience in mussels against climate change conditions, strengthen biosecurity management programs, improve farming and processing efficiency, and add value in terms of market-desirable traits. These data hold promise for advancing sustainability efforts within the aquaculture industry. Graphical abstract

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Energy reserves and gut microbiota of marine mussels under combined exposure to pathogens and predation risk

Liu,

Chen,

et al. 2025

Aquaculture

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Effects of seawater temperature and acute Vibrio sp. challenge on the haemolymph immune and metabolic responses of adult mussels (Perna canaliculus)

Cited by 13 publications

References 81 publications

Investigating the effect of bacterial coinfections on juvenile and adult green‐lipped mussels (Perna canaliculus)

Investigating the effect of bacterial coinfections on juvenile and adult green‐lipped mussels (Perna canaliculus)

Biomarkers of mussel exposure to Vibrionaceae: A review

Energy reserves and gut microbiota of marine mussels under combined exposure to pathogens and predation risk

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