Effects of second generation tetracyclines on penicillin-epilepsy-induced hippocampal neuronal loss and motor incoordination in rats

Yılmaz, İsmail; Adiguzel, Esat; Akdoğan, Ilgaz; Kaya, Ertuğrul; Hatip‐Al‐Khatib, Izzettin

doi:10.1016/j.lfs.2006.02.027

Cited by 9 publications

(5 citation statements)

References 51 publications

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“…In line with that reported in our previous study (53), generalization of the seizures after 30-40 min and The neuron number significantly decreased in the penicillin+nifedipine group compared with the control and penicillin groups, respectively (p<0.05 for both; Mann Whitney U).…”

Section: Discussionsupporting

confidence: 92%

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Effect of nifedipine on hippocampal neuron number in penicillin-induced epileptic rats

Yılmaz

Akdogan²,

Kaya³

et al. 2013

Turkish Neurosurgery

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AIm: Epileptic seizures lead to neuronal loss in the hippocampus. Experimental epilepsy can be induced by direct application of various chemicals to cerebral cortex. Nifedipine is an L-type voltage-dependent calcium channel blocker. In spite of several studies that show the seizure-suppressing effects of nifedipine, it has been shown that nifedipine does not suppress but conversely increases epileptic seizures. Similarly, contradictory effects of nifedipine have been reported, such as neuroprotection, failed neuroprotection and neurotoxicity. We therefore aimed to investigate the effect of nifedipine on hippocampal neuronal loss in penicillin induced epileptic rats in this study. mATeRIAL and meTHods:The effect of nifedipine on total hippocampal neuron number was estimated by using the optical fractionator method (an unbiased stereological method) in penicillin-G induced epileptic rats. ResuLTs:The total number of hippocampal neurons in the control group was 183687 ± 3184. In the penicillin-induced group, the total neuron number significantly decreased to 146318 ± 3042 compared to the control group. In the nifedipine group, the neuron number significantly decreased to 128873 ± 1157 compared to both control and penicillin-induced groups.CoNCLusIoN: Nifedipine increased neuronal loss and did not suppress epileptic seizures in penicillin-induced epileptic rats. Nifedipine could not protect against hippocampal neuronal loss in penicillin-induced epileptic rats.KeywoRds: Penicillin epilepsy model, Nifedipine, Hippocampus, Neuron number, The optical fractionator method, Rat, Epilepsy, Seizure ÖZ AmAÇ: Epileptik nöbetler hipokampusta nöron kaybına yol açmaktadır. Çeşitli kimyasalların beyin korteksine direkt olarak uygulanmasıyla deneysel epilepsi oluşturulabilir. Nifedipin, L-tipi voltaj bağımlı kalsiyum kanal blokörüdür. Nifedipinin nöbet baskılayıcı etkilerini gösteren çeşitli çalışmalar bulunduğu gibi, epileptik nöbetleri baskılamadığı tam tersine arttırdığı da gösterilmiştir. Benzer şekilde, nifedipinin çelişkili etkileri bildirilmiştir; olumlu -olumsuz nöroprotektif (nöron koruyucu) ve nörotoksisite gibi. Bu nedenle, bu çalışmada, penisilin ile deneysel epilepsi oluşturulan sıçanlarda nifedipinin hipokampal nöron kaybı üzerine etkisini araştırmayı amaçladık. yÖNTem ve GeReÇLeR: Nifedipinin penisilin G ile deneysel epilepsi oluşturulan sıçanlarda toplam hipokampal nöron sayısına etkisi tarafsız (objektif ) bir stereolojik metot olan optik parçalama yöntemi ile değerlendirildi.BuLGuLAR: Kontrol grubunun toplam hipokampal nöron sayısı 183687 ± 3184 idi. Penisilin grubunun toplam nöron sayısı kontrol grubuna göre anlamlı olarak düşüktü (146318 ± 3042). Penisilin+nifedipin grubunun toplam nöron sayısı (128873 ± 1157) hem kontrol hem de penisilin grubuna göre istatistiksel olarak anlamlı olarak azalmış bulundu. soNuÇ: Nifedipinin epileptik sıçanlarda nöron kaybını arttırdığı ve epileptik nöbetleri baskılamadığı ortaya kondu. Nifedipin, penisilin ile deneysel epilepsi oluşturulan sıçanlarda ortaya çıkan nöron ka...

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Section: Discussionsupporting

confidence: 92%

“…All procedures in the present study were conducted according to a protocol approved by the ethical committee. All efforts were made to minimize animal suffering and to reduce the number of animals used (53).…”

Section: Animalsmentioning

confidence: 99%

Effect of nifedipine on hippocampal neuron number in penicillin-induced epileptic rats

Yılmaz

Akdogan²,

Kaya³

et al. 2013

Turkish Neurosurgery

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“…Minocycline, a second-generation tetracycline, has antiinflammatory, antioxidative and antiapoptotic effects (Domercq and Matute, 2004;Zemke and Majid, 2004;Elewa et al, 2006) that makes it a promising drug for the treatment of various CNS diseases, such as experimental models of focal cerebral ischemia (Yrjanheikki et al, 1999), vascular dementia (Cho et al, 2006b), Parkinson's disease (Du et al, 2001), amyotrophic lateral sclerosis (Zhu et al, 2002), and epilepsy (Yilmaz et al, 2006). In particular, minocycline has been found to be protective in rat (Yrjanheikki et al, 1999;Wang et al, 2003;Hewlett and Life Sciences 80 (2007) 2030-2035 www.elsevier.com/locate/lifescie Corbett, 2006) and mouse (Koistinaho et al, 2005;Yenari et al, 2006) models of focal forebrain ischemia.…”

Section: Introductionmentioning

confidence: 99%

Regional differences in the neuroprotective effect of minocycline in a mouse model of global forebrain ischemia

et al. 2007

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“…Pyramidal cells in the hippocampus are highly sensitive to hypoxia-ischemia [22]. Transient ischemia leads to damage in specific areas of the brain, such as CA1 area, resulting in neuron death.…”

Section: Discussionmentioning

confidence: 99%

Presentation of the change in the number of hippocampal neurons by stereological method in surviving cases of mechanical asphyxia: An experimental rat study

Cenger¹,

Kurtulus²,

Acar³

et al. 2012

RJLM

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Asphyxia is the most common death cause in Forensic Medicine. Strangulations do not always result in death. Cases recovering from asphyxia have many disturbances in different organs because of ischemia. In this study, using stereological methods, after asphyxia was performed by strangulation, the mean number of pyramidal neurons in hippocampus was aimed to evaluate. Rats are divided into two groups. The first one was the control group and the other one was asphyxiated by strangulation. The brains of strangulated group rats were extracted after seven days. Sections were taken by cryostat with the method of "Systematic Randomised Sample Strategy" and stained with hematoxylen-eosin. In these sections, total pyramidal neuronal numbers in hippocampus were determined by the "Optical Fractioning Method". In our study, the mean cell number of asphyxiated group (158985,2±17439,8) was significantly less in the control group (265689,4±39009,5) (p=0.009). In conclusion, strangulation-induced asphyxia is resulted in hippocampal neuronal lost.

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Effects of second generation tetracyclines on penicillin-epilepsy-induced hippocampal neuronal loss and motor incoordination in rats

Cited by 9 publications

References 51 publications

Effect of nifedipine on hippocampal neuron number in penicillin-induced epileptic rats

Effect of nifedipine on hippocampal neuron number in penicillin-induced epileptic rats

Regional differences in the neuroprotective effect of minocycline in a mouse model of global forebrain ischemia

Presentation of the change in the number of hippocampal neurons by stereological method in surviving cases of mechanical asphyxia: An experimental rat study

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