Effects of selected polychlorinated biphenyl (PCB) congeners on hepatic glutathione, glutathione-related enzymes, and selenium status: implications for oxidative stress☆1☆Contents reflect the views of the authors and do not represent any official view(s) of NIEHS, EPA, or DOD.1Abbreviations: PCBs, polychlorinated biphenyls; GST, glutathione transferase; GPX, glutathione peroxidase; SeGPX, selenium-dependent glutathione peroxidase; GR, glutathione reductase; MOPS, 3-{N-morpholino}propane sulfonic acid buffe

Twaroski, Timothy P.; O’Brien, Michelle L.; Robertson, Larry W.

doi:10.1016/s0006-2952(01)00668-2

Cited by 92 publications

(13 citation statements)

References 43 publications

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“…Previous studies also report decreased levels of hepatic selenium following PCB77 [26] and TCDD treatments [27]. PCB77 induced decrease in selenium was associated with a decrease in rat liver Se-GPx1 activity and gpx1 mRNA levels [26]. These previous literature reports and results presented here suggest that PCB induced decrease in selenoprotein expression could be a major mechanism of PCB-induced toxicity.…”

Section: Discussionsupporting

confidence: 83%

“…However, a recent study reports decreased hepatic levels of selenium and zinc in PCB126 treated rat livers correlating with a decrease in Se-GPx1 activity [25]. Previous studies also report decreased levels of hepatic selenium following PCB77 [26] and TCDD treatments [27]. PCB77 induced decrease in selenium was associated with a decrease in rat liver Se-GPx1 activity and gpx1 mRNA levels [26].…”

Section: Discussionmentioning

confidence: 99%

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Selenoprotein P regulates 1-(4-Chlorophenyl)-benzo-2,5-quinone-induced oxidative stress and toxicity in human keratinocytes

Xiao

Zhu

Sarsour

et al. 2013

Free Radical Biology and Medicine

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Polychlorinated biphenyls and their metabolites are environmental pollutants that are believed to have adverse health effects presumably by inducing oxidative stress. To determine if 1-(4-Chlorophenyl)-benzo-2,5-quinone (4-ClBQ: metabolite of 4-monochlorobiphenyl, PCB3) induced oxidative stress is associated with changes in the expression of specific antioxidant genes, mRNA levels of 92 oxidative stress-response genes were analyzed using TaqMan® Array Human Antioxidant Mechanisms (Life technologies), and results were verified by performing quantitative RT-PCR assays. The expression of selenoprotein P (sepp1) was found to be significantly downregulated (8–10-fold) in 4-ClBQ treated HaCaT human skin keratinocytes, which correlated with a significant increase in MitoSOX oxidation. Overexpression of Mn-superoxide dismutase, catalase, or treatment with N-acetyl-L-cysteine suppressed 4-ClBQ-induced toxicity. Sodium selenite supplementation also suppressed 4-ClBQ-induced decrease in sepp1 expression, which was associated with a significant inhibition in cell death. Furthermore, HaCaT cells overexpressing sepp1 were resistant to 4-ClBQ induced oxidative stress and toxicity. These results demonstrate that SEPP1 represents a previously unrecognized regulator of PCB induced biological effects. These results support the speculation that selenoproteins can be an attractive countermeasure for PCB induced adverse biological effects.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Selenoprotein P regulates 1-(4-Chlorophenyl)-benzo-2,5-quinone-induced oxidative stress and toxicity in human keratinocytes

Xiao

Zhu

Sarsour

et al. 2013

Free Radical Biology and Medicine

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“…Twaroski et al [41] indicated that toxic manifestation induced by PCB may associate with enhanced production of ROS and thereby induce oxidative stress through the initiation of self-propagating LPO reaction. A study showed that hippocampus is more vulnerable to tertbutyl hydroperoxide t-BuOOH-induced oxidative insult than mid brain.…”

Section: Discussionmentioning

confidence: 99%

Polychlorinated Biphenyls-Induced Oxidative Stress on Rat Hippocampus: A Neuroprotective Role of Quercetin

Selvakumar

Bavithra

Giribabu

et al. 2012

The Scientific World Journal

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Present study is aimed to evaluate the ameliorative role of quercetin on PCBs-induced oxidative stress in hippocampus of Wistar rats. Group I rats received vehicle (corn oil) intraperitoneally (i.p); Group II received quercetin 50 mg/kg bwt/day (gavage); Group III received PCB 2 mg/kg bwt/day (i.p); Group IV received PCB (i.p) and simultaneously quercetin through gavage. After 30 days, rats were euthanized and hippocampus was dissected from each rat brain. Oxidative stress was assessed by determining the levels of H2O2, LPO, Pcc, and alteration in the functional markers such as CK, AchE, and ATPases activities in the hippocampus of control and experimental animals. A significant increase in the levels of stress markers and decrease in level of functional markers were observed in PCBs-treated rats. Moreover DNA fragmentation and histological studies were ascertained to confirm PCBs toxicity. In conclusion, quercetin shows a protective role against PCBs-induced oxidative damage in rat hippocampus.

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“…29,30,38,73,74 Fadhel et al 38 and Lamartinier et al 73 observed a significant increase in glutathione- S -transferase (GST) in Sprague–Dawley rats that were exposed to 150 μ mol/kg PCB 153 for six days and an increase in hepatic lipid peroxidation with a single dose of PCB 153. Twaroski et al 30 also reported similar findings from rats that received intraperitoneal injections of PCB 153 (100 mmol/kg for 3 wks). Previous reports also showed Sprague–Dawley rats to have significant increases in oxidized vitamin E ( α -tocopheryl quionone) after intraperitoneal PCB 153 injections (100 mmol/kg/injection) 75 as well as a significant induction of hepatic vitamin A following subchronic exposure to 50 ppm of PCB 153.…”

Section: Resultsmentioning

confidence: 99%

Polychlorinated Biphenyls Induce Oxidative DNA Adducts in Female Sprague–Dawley Rats

Mutlu

Gao

Collins

et al. 2016

Chem. Res. Toxicol.

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Polychlorinated biphenyls (PCBs) are organic chemicals that were traditionally produced and widely used in industry as mixtures and are presently formed as byproducts of pigment and dye manufacturing. They are known to persist and bioaccumulate in the environment. Some have been shown to induce liver cancer in rodents. Although the mechanism of the toxicity of PCBs is unknown, it has been shown that they increase oxidative stress, including lipid peroxidation. We hypothesized that oxidative stress-induced DNA damage could be a contributor for PCB carcinogenesis and analyzed several DNA adducts in female Sprague–Dawley rats exposed to 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126), 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB 153), and a binary mixture (PCB 126 + 153) for 14, 31, and 53 wks. Eight adducts were measured to profile oxidative DNA lesions, including 8-oxo-deoxyguanosine (8-oxo-dG), 1,N6-ethenodeoxyadenosine (1,N6-εdA), N2,3-ethenoguanine (N2,3-εG), 1,N2-ethenodeoxyguanosine (1,N2-εdG), as well as malondialdehyde (M1dG), acrolein (AcrdG), crotonaldehyde (CrdG), and 4-hydroxynonenal-derived dG adducts (HNEdG) by LC–MS/MS analysis. Statistically significant increases were observed for 8-oxo-dG and 1,N6-εdA concentrations in hepatic DNA of female rats exposed to the binary mixture (1000 ng/kg/day + 1000 μg/kg/day) but not in rats exposed to PCB 126 (1000 ng/kg/day) or PCB 153 (1000 μg/kg/day) for 14 and 31 wks. However, exposure to PCB 126 (1000 ng/kg/day) for 53 wks significantly increased 8-oxo-dG, 1,N6-εdA, AcrdG, and M1dG. Exposure to PCB 153 (1000 μg/kg/day) for 53 wks increased 8-oxo-dG, and 1,N6-εdA. Exposure to the binary mixture for 53 wks increased 8-oxo-dG, 1,N6-εdA, AcrdG, 1,N2-εdG, and N2,3-εG significantly above control groups. Increased hepatic oxidative DNA adducts following exposure to PCB 126, PCB 153, or the binary mixture shows that an increase in DNA damage may play an important role in hepatic toxicity and carcinogenesis in female Sprague–Dawley rats.

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Cited by 92 publications

References 43 publications

Selenoprotein P regulates 1-(4-Chlorophenyl)-benzo-2,5-quinone-induced oxidative stress and toxicity in human keratinocytes

Selenoprotein P regulates 1-(4-Chlorophenyl)-benzo-2,5-quinone-induced oxidative stress and toxicity in human keratinocytes

Polychlorinated Biphenyls-Induced Oxidative Stress on Rat Hippocampus: A Neuroprotective Role of Quercetin

Polychlorinated Biphenyls Induce Oxidative DNA Adducts in Female Sprague–Dawley Rats

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