Effects of Selenium and Vitamin E on Blood Selenium Levels, Tissue Glutathione Peroxidase Activities and White Muscle Disease in Sheep Fed Purified or Hay Diets

Whanger, P. D.; Weswig, P. H.; Schmitz, J. A.; Oldfield, J. E.

doi:10.1093/jn/107.7.1298

Cited by 81 publications

(28 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…20 The standard reaction mixture was composed of 1.0 ml of 50 mM phosphate buffer (pH 7.0) containing 4 mM EDTA, 0.2 ml of 10 mM sodium azide, 0.1 ml of Drapkin's solution, 0.2 ml of 0.01 M reduced glutathione, 0.04 ml of 7.5 mM NADPH, 0.03 ml of glutathione reductase (6 U/ml), 0.32 ml of distilled water, and 0.1 ml of sample. All components were mixed together and incubated at 25°C for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

Biological defence mechanism in the pathogenesis of prolonged cerebral vasospasm in the patients with ruptured intracranial aneurysms.

Sakaki¹,

Kuwabara²,

Ohta³

1986

Stroke

View full text Add to dashboard Cite

SUMMARY We examined the relationship between the biological protective mechanisms of scavengers and free radicals that are elicited by subarachnoid hemorrhage (SAH) hi the pathogenesis of prolonged vasospasm following ruptured intracranial aneurysm. The study included 25 patients treated by early surgery (within 72 hours after SAH). Lipid peroxides concentrations and the activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-px) in the cerebrospinal fluid (CSF) were measured. The concentration of lipid peroxides increased significantly more (p < 0.05) during the first 4 days after SAH in patients with symptomatic vasospasm than in those without. Patients with symptomatic vasospasm had a marked decrease in SOD activity on Days 3 and 4 followed by a gradual decrease, whereas the patients without spasm showed little change (difference between the groups, p < 0.05). There was a significant difference in catalase activity reversal to SOD activity, but no difference in GSH-px activity. Thus, correlation was close between the increased lipid peroxides concentration and the decrease in SOD activity in CSF (p < 0.05), suggesting an important mechanism in the pathogenesis of vasospasm. Stroke Vo! 17, No 2, 1986 AMONG THE MANY FACTORS PROPOSED as causes of prolonged cerebral vasospasm following ruptured intracranial aneurysms, subarachnoid blood is one obvious one.1 3 Recently, free radical pathology has been discussed in the pathogenesis of prolonged cerebral vasospasm.4 " 6 Subarachnoid hemorrhage (SAH) gives rise to a specific environment around the cerebral arteries. There is an abundance of oxyhemoglobin, which can generate superoxide during autoxidation, 7 -8 and also of polyunsaturated fatty acids derived from the lysis of erythrocyte membranes; those fatty acids autoxidize readily to form lipid peroxides.9 ' l0 Generation of more active species of oxygen on the surface of leucocytes during their phagocytotic activities may also be important." Activated products such as active species of oxygen, lipid peroxides and other free radicals are likely to cause functional and morphological damage to cells in the arterial wall through peroxidation of membrane phospholipids or enzymes.12 -l3 Against such damage, there are a variety of biological protective systems.8 -14~16 In normal cells, superoxide radical anion is broken down by superoxide dismutase (SOD), and the hydrogen peroxide that is a product of this reaction is broken down by catalase and glutathione peroxidase (GSH-px). These enzymes may be important protectors against lipid peroxidation damage to cerebral arteries after the onset of SAH.There are a number of reports in which the peroxidation of polyunsaturated fatty acids by active species of oxygen or hydroxy radicals and its inhibition by SOD, catalase, and GSH-px were studied. However, there have been no thorough studies of these enzymes protection against peroxidation damage in patients with ruptured intracranial aneurysms.From the Department of Neurological Surgery, Ehime Uni...

show abstract

Section: Methodsmentioning

confidence: 99%

Biological defence mechanism in the pathogenesis of prolonged cerebral vasospasm in the patients with ruptured intracranial aneurysms.

Sakaki¹,

Kuwabara²,

Ohta³

1986

Stroke

View full text Add to dashboard Cite

show abstract

“…Deficiencies of either alpha-tocopherol or selenium also occur naturally. For example, White muscle disease (WMD) is seen in farm animals and arises as a consequence of a combination of inadequate soil selenium and enhanced oxidation of alpha-tocopherol in foodstuffs or diets poor in alpha-tocopherol (12)(13)(14)(15)(16)(17)(18)(19)(20). In animals with WMD, alpha-tocopherol and selenium contents of affected muscle are reduced with consequential reductions in glutathione peroxidase in plasma (15).…”

Section: Effects Of Selenium and Alpha-tocopherol Deficiencies On Skementioning

confidence: 99%

Oxidants antioxidants and alcohol implications for skeletal and cardiac muscle

Preedy¹

1999

Front Biosci

View full text Add to dashboard Cite

“…They concluded that vitamin A, like vitamin E, helps maintain normal blood parameters in physiological levels and can be also used prophylactically. Vitamin E alone has been reported to be more effective than selenium alone in the prevention of WMD (17). A ewe can receive vitamin E prior to lambing.…”

Section: Resultsmentioning

confidence: 99%

Retinol, α-tocopherol and vitamin D3 in White MuscleDisease

Mert

Yıldırım

Yörük

et al. 2018

Medycyna Weterynaryjna

View full text Add to dashboard Cite

Vitamins are essential for the health of all living organisms. Vitamins E, A, D and K are known as fat-soluble vitamins, and deprivation of vitamin E causes various disorders, especially in the reproduction and cardiovascular systems and in muscle functions. Vitamin A, on the other hand, has roles in various biological functions – like eyesight – and the growth, reproduction and differentiation of epithelial cells. Vitamin A deficiency leads to the keratinization of the epithelium, and disorders related to the metaplasies of the genital and genitourinary systems. Conversely, vitamin D is defined as a pro-hormone and is responsible for Cahomeostasis, and thus indirectly affects the bone metabolism, bone structure, and cellular and neural functions of Ca. White muscle disease (WMD) can occur in newborn lambs, but is more commonly seen in lambs of up to 3 months of age. In this study, 30 lambs of 3 to 50-days-old from different flocks diagnosed with White Muscle Disease (WMD) were selected as research material, while the control group consisted of 8 healthy lambs. With the aim of clarifying the cause of WMD, serum fat-soluble vitamins, retinol, α-tocopherol and vitamin D3 levels were determined in 16 lambs. Gluteal and heart musclet issue samples also were taken from 30 lambs with WMD. The vitamin levels of the samples were analysed by HPLC. The levels of serum α-tocopherol, retinols, and vitamin D3 were foundto be low in the diseased animals, but only retinol (p<0.001) and α-tocopherol (p<0.0011) level differences were statistically relevant. Macroscopically, Zenker’s necrosis was determined in the heart muscles of 17 lambs, and in the gluteal and chest muscles of 6 lambs. 7 lambs displayed necrosis in both their heart and in gluteal muscles. The samples were analyzed microscopically to reach similar findings: swollen homogeneous pink muscles, pycnotic nuclei, and hyperaemic and haemorrhagic blood vessels in gluteal, chest and heart muscles. Hyaline degeneration and Zenker's necrosis, dystrophic regions in necrotic areas, cc was detected as a severe disease in lambs at an early stage of life with advanced degeneration in different muscle tissues. Deficiency of fat-soluble vitamins was also detected in the sick animals. Control group lambs had higher levels of α tocopherol and retinol (p<0.001) compared to the sick lambs. .

show abstract

Effects of Selenium and Vitamin E on Blood Selenium Levels, Tissue Glutathione Peroxidase Activities and White Muscle Disease in Sheep Fed Purified or Hay Diets

Cited by 81 publications

References 18 publications

Biological defence mechanism in the pathogenesis of prolonged cerebral vasospasm in the patients with ruptured intracranial aneurysms.

Biological defence mechanism in the pathogenesis of prolonged cerebral vasospasm in the patients with ruptured intracranial aneurysms.

Oxidants antioxidants and alcohol implications for skeletal and cardiac muscle

Retinol, α-tocopherol and vitamin D3 in White MuscleDisease

Contact Info

Product

Resources

About