Effects of Selenium Supplementation on the Ion Homeostasis in the Reproductive Organs and Eggs of Laying Hens Fed With the Diet Contaminated With Cadmium, Lead, Mercury, and Chromium

Abstract

The objective of this study was to explore the toxic effects of different heavy metals in combination with their deposition and ion homeostasis in the reproductive organs and eggs of laying hens, as well as the alleviating action of selenized yeast. A total of 160 Lohmann pink-shell laying hens (63-week-old) were randomly allocated into four treatments with 10 replicates of four hens each. The four dietary treatments were the corn-soybean meal basal dietary (control; CON); the CON dietary supplemented with 0.4 mg/kg selenium from selenized yeast (Se); the CON dietary supplemented with 5 mg/kg Cd + 50 mg/kg Pb +3 mg/kg Hg + 5 mg/kg Cr (HEM), and the HEM dietary supplemented with 0.4 mg/kg selenium from selenized yeast (HEM+Se). The dietary HEM significantly increased Cd, Pb, and Hg deposition in the egg yolk and ovary, and Cd and Hg deposition in the oviduct and in the follicular wall (p < 0.05). The HEM elevated Fe concentration in the egg yolk, ovary, and oviduct (p < 0.05). The HEM decreased Mn concentration in the egg yolk, Fe, Mn, and Zn concentrations in the egg white, Cu concentration in the ovary, Mg concentration in the oviduct, as well as Ca, Cu, Zn, and Mg concentrations in the follicular walls (p < 0.05). Dietary Se addition elevated Se concentration in the egg yolk, oviduct, and follicular walls and Mg concentration (p < 0.05) in the oviduct, whereas it reduced Fe concentration in the oviduct compared with the HEM-treated hens. Some positive or negative correlations among these elements were observed. Canonical Correlation Analysis showed that the concentrations of Pb and Hg in the egg yolk were positively correlated with those in the ovary. The concentration of Cd in the egg white was positively correlated with that in the oviduct. In summary, dietary Cd, Pb, Hg, and Cr in combination caused ion loss and deposition of HEM in reproductive organs of laying hens. Dietary Se addition at 0.4 mg/kg from selenized yeast alleviated the negative effects of HEM on Fe and Mg ion disorder in the oviduct and follicle wall of hens.

Protective effects of selenized yeast on the combination of cadmium-, lead-, mercury-, and chromium-induced toxicity in laying hens

“…The R 2 values of standard curves for the different heavy metals were higher than 0.99. The recovery rates of Cd, Pb, Cr, Hg, and Se were 91.8–98.4%, 93.9–97.4%, 96.9–100.3%, 97.9–103.7%, and 92.9–102.1%, respectively ( 44 ).…”

“…Therefore, to construct the laying hen model of sub-chronic heavy metal intoxication, the addition of 5 mg/kg Cd, 50 mg/kg Pb, 3 mg/kg Hg, and 5 mg/kg Cr approximates the 10-fold of the limits of national standards, were chosen in this study. In addition, our previous research has indicated that the HEM dosages have disturbed the ion balance of reproductive organs of laying hens ( 44 ). Meanwhile, the maximum permitted limits of Se in poultry feed are 0.5 mg/kg, based on the Se content 1.4 mg/kg in basal dietary, 0.4 mg/kg Se from selenized yeast was used in this study.…”

Tannic Acid and Tea Prevents the Accumulation of Lead and Cadmium in the Lungs, Heart and Brain of Adolescent Male Wistar Rats—Possible Therapeutic Option

“…Since it is impossible to eliminate Pb and Cd from food, research is carried out on methods of limiting its absorption by the body. These methods should be easily accessible and uncomplicated [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Foods containing significant amounts of antioxidant components can be used in the daily diet to prevent the dangerous effects of toxic metals on the human body [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ].…”