Effects of Selenylation Modification on Antioxidative Activities of Schisandra chinensis Polysaccharide

Yue, Chanjuan; Chen, Jin; Hou, Ranran; Liu, Jie; Li, Xiuping; Gao, Zhenzhen; Liu, Cui; Wang, Deyun; Lu, Yu; Li, Hongquan; Hu, Yuanliang

doi:10.1371/journal.pone.0134363

Cited by 33 publications

(14 citation statements)

References 40 publications

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“…We determined the ABTS• + scavenging ability of PSPs, PSPs-EH20 and PSPs-EH80 (the scavenging activity at 0.6 mg/mL is 70%, 90% and 100%, respectively) and found that the IC 50 of PSPs-EH80 ABTS• + scavenging ability was 0.14 mg/mL, which is lower than that of PSPs-EH20 (IC 50 = 0.16 mg/mL) and PSPs (Figure 2). Furthermore, the PSPs-EH80 ABTS• + scavenging ability has a better result than that of the floral mushroom and Schisandra chinensis polysaccharide [19,20]. This result suggests that PSPs-EH80 has a greater ability than PSPs-EH20 and PSPs to neutralize free radicals by accepting or donating an electron to eliminate the unpaired condition due to PSPs-EH80 having a low molecular weight [9].…”

Section: Resultsmentioning

confidence: 99%

Enhancing the Antioxidant Ability of Trametes versicolor Polysaccharopeptides by an Enzymatic Hydrolysis Process

et al. 2016

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Polysaccharopeptides (PSPs) are among the main bioactive constituents of Trametes versicolor (T. versicolor). The purpose of this research was to investigate the antioxidant activities of enzymatic hydrolysates obtained from T. versicolor polysaccharopeptides by 80 U/mL β-1,3-glucanase (PSPs-EH80). The half-inhibitory concentration (IC 50 ) of PSPs-EH80 in metal chelating assay, ABTS and DPPH radical scavenging test results were 0.83 mg/mL, 0.14 mg/mL and 0.52 mg/mL, respectively, which were lower than that of PSPs-EH 20 U/mL. The molecular weights of the PSPs-EH80 hydrolysates were 300, 190, 140 and 50 kDa, respectively, and the hydrolysis of polysaccharides by β-1,3-glucanase did not change the original functional group. PSPs-EH80 reduced the reactive oxygen species (ROS) content at least twice that of treatment without PSPs-EH80. In addition, an oxidative damage test showed that PSPs-EH80 can improve HaCaT cell survival. According to our results, PSP demonstrates the potential of anti-oxidative damage; besides, enzyme hydrolysis can improve the ability of the PSP.

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Section: Resultsmentioning

confidence: 99%

Enhancing the Antioxidant Ability of Trametes versicolor Polysaccharopeptides by an Enzymatic Hydrolysis Process

et al. 2016

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“…20 MDA is the end product of lipid peroxidation, and the content of MDA is an indicator of free radical levels. 21 SOD with clearing O 2 and preventing tissue injury by toxic oxygen-free radicals can be an indicator of antioxidant activity. 22 Therefore, the level of SOD and MDA are important biomarkers of oxidative stress status.…”

Section: Discussionmentioning

confidence: 99%

Anti-oxidative and neuroprotective effects of flaxseed on experimental unilateral spinal cord injury in rat

2017

J. contemp. med. sci.

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Objectives Investigation of anti-oxidative and neuroprotective effects of flaxseed on rats with unilateral SCI. Methods 30 male Wistar rats were randomly assigned to five groups: control (Ctrl), laminectomy, flaxseed, spinal cord injured (SCI) and SCI + flaxseed (treatment) groups. SCI model was induced by placing a 50 g weight for 5 min on to a 2.2 mm × 2.5 mm platform applied to the dura at vertebral level T10. After 4 weeks, the blood of rats in different groups was collected and the influence of flaxseed on antioxidant enzymes and oxidative stress marker level, histologic alterations and locomotion score were assessed. Results Our results showed that in the SCI group, the mean level of Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were significantly decreased (P < 0.01), whereas the mean malondialdehyde (MDA) content was significantly increased (P < 0.001) in comparison to the laminectomy group. Furthermore, the mean levels of SOD, GPx and CAT in the treatment group was higher than the SCI group (P < 0.001), while, the mean MDA content in the treatment group was significantly less than the SCI group (P < 0.001). In addition, comparison between SCI and treatment groups determined a significant decrease in tissue degeneration and volume of cavities in the treatment group. However, Basso, Beattie and Bresnahan (BBB) scores were significantly increased in flaxseed-treated rats on days 14 (P < 0.05), 21 and 28 (P < 0.01) after surgery compared with the SCI group. Conclusions Our study for the first time showed the anti-oxidative and neuroprotective effects of flaxseed on experimental unilateral spinal cord injury in rat.

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“…18 GSH is a major intracellular antioxidant that evades free radicals and catalyses the dismutation of superoxide anions. 19 The level of GSH reflects the ability to scavenge toxic free radicals.…”

Section: Discussionmentioning

confidence: 99%

Effects of calcitriol on experimental spinal cord injury in rats

Jin

et al. 2016

Spinal Cord

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Study design: Experimental, controlled, animal study. Objectives: To evaluate the effects of calcitriol on oxidative stress, apoptosis, autophagy and locomotor recovery in rats after spinal cord injury (SCI). Setting: China. Methods: Ninety female rats were randomly divided into three groups. Laminectomy only was performed in the control group. The SCI group received laminectomy as well as spinal cord compression injury. In the calcitriol group, SCI rats received an intraperitoneal injection of calcitriol (2 μg kg − 1 day − 1 ). Oxidative stress was assessed by the tissue superoxide dismutase (SOD) activity and the contents of glutathione (GSH) and malondialdehyde (MDA). The extent of apoptosis was assessed by immunohistochemistry for C-caspase3, TUNEL staining and western blotting for C-caspase3, Bax and Bcl2. Transmission electron microscopy was used to examine autophagosomes in the injured spinal cord of calcitriol-treated rats. Autophagy was detected by western blotting for LC3-II, Beclin1 and p62. Histological changes were assessed by haematoxylin and eosin staining and Nissl staining. Functional recovery was reflected by the Basso, Beattie and Bresnahan locomotion rating scale and the inclined plane test. Results: With calcitriol treatment, oxidative stress was decreased, SOD activity and GSH content were increased and MDA content was decreased. Moreover, apoptosis was inhibited in the SCI plus calcitriol group. However, a higher level of autophagy was detected in the lesions of the calcitriol group compared with the SCI group. Histological damage and neuron loss after SCI were reduced in calcitrioltreated rats, and functional recovery was significantly promoted in the calcitriol group compared with controls. Conclusions: Calcitriol promotes locomotor recovery after SCI by reducing oxidative stress and inhibiting apoptosis, as well as promoting autophagy.

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Effects of Selenylation Modification on Antioxidative Activities of Schisandra chinensis Polysaccharide

Cited by 33 publications

References 40 publications

Enhancing the Antioxidant Ability of Trametes versicolor Polysaccharopeptides by an Enzymatic Hydrolysis Process

Enhancing the Antioxidant Ability of Trametes versicolor Polysaccharopeptides by an Enzymatic Hydrolysis Process

Anti-oxidative and neuroprotective effects of flaxseed on experimental unilateral spinal cord injury in rat

Effects of calcitriol on experimental spinal cord injury in rats

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