Effects of Sequence and Base Composition on the CD and TDS Profiles of i‐DNA

Abstract

The i-motif DNA, also knowna si -DNA, is an oncanonical DNAs econdary structure formed by cytosine-rich sequences,c onsisting of two intercalated parallel-stranded duplexes held together by hemi-protonated cytosine-cytosine + (C:C + )base pairs.The growing interest in the i-DNAstructure as atarget in anticancer therapyincreases the need for tools for arapid and meaningful interpretation of the spectroscopic data of i-DNAsamples.Herein, we analyzed the circular dichroism (CD) and thermal difference UV-absorbance spectra (TDS) of 255 DNAs equences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i-DNA-forming sequences.

Thermal and pH Stabilities of i‐DNA: Confronting in vitro Experiments with Models and In‐Cell NMR Data

“…Multivariate data analysis for the interpretation of TDS and CD spectra for these sequences are provided in our companion paper. [29] Even if impressive,t his dataset does not allow to explore the full sequence space of i-DNA-prone sequences.D espite these restrictions,a nd because we tested af ew sequences escaping this sequence space,o ur data already provides key information on i-DNAs tability.…”