Effects of serum and serum heat-inactivation on human bone derived osteoblast progenitor cells

Bruinink, A.; Tobler, Ursina; Hälg, M; Grunert, Julia

doi:10.1023/b:jmsm.0000021127.62879.a1

Cited by 34 publications

(31 citation statements)

References 18 publications

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“…In addition, human but not rodent MSCs have been shown to senesce in culture (Digirolamo et al, 1999;Javazon et al, 2001). Clearly, the methods used to amplify MSCs in culture may in part account for the variations since the growth and differentiation of MSCs can be profoundly affected by various serum components, growth factors, and culture conditions (Tsutsumi et al, 2001;Bruinink et al, 2004;Moussavi-Harami et al, 2004). Some batches of the porcine MSCs were found to be able to go through more than 100 population doublings in 40 trypsin passages without losing their ability to proliferate when maintained in the ADMEM medium.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture

et al. 2005

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The in vitro culture of porcine bone marrow-derived mesenchymal stem cells (MSCs) was used for the investigation of adult stem cell biology. Isolated porcine MSCs possessed the ability to proliferate extensively in an antioxidants-rich medium containing 5% fetal bovine serum (FBS). Greater than 40 serial MSC passages and 100 cell population doublings have been recorded for some MSC batches. Early and late passage MSCs were defined here as those cultures receiving less than 5 trypsin passages and more than 15 trypsin passages, respectively. Consistent with their robust ability to proliferate, both the early and late passage MSCs expressed the cell-cycle promoting enzyme p34cdc2 kinase. Late MSCs, however, exhibited certain features reminiscent of cellular aging such as actin accumulation, reduced substrate adherence, and increased activity of lysosomal acid beta-galactosidase. Early MSCs retained the multipotentiality capable of chondrogenic, osteogenic, and adipogenic differentiation upon induction in vitro. In contrast, late MSCs were only capable of adipogenic differentiation, which was greatly enhanced at the expense of the osteochondrogenic potential. Along with these changes in multipotentiality, late MSCs expressed decreased levels of the bone morphogenic protein (BMP-7) and reduced activity of alkaline phosphatase. Late MSCs also exhibited attenuated synthesis of the hematopoietic cytokines granulocyte colony-stimulating factor (G-CSF), leukemia inhibitory factor (LIF), and stem cell factor (SCF). The long-term porcine MSC culture, thus, provides a model system to study the molecular interplay between multiple MSC differentiation cascades in the context of cellular aging.

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Section: Discussionmentioning

confidence: 99%

Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture

et al. 2005

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“…) 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 9 Primary human bone marrow mesenchymal stromal stem cells (HBMCs) were obtained from the bone marrow of femur head of four patients (3 females and 1 male, 66-79 years old) undergoing hip replacement surgery after informed consent. The cells were isolated and expanded as previously described [25]. HBMC used in experiments were from passage number 1 and 2 and each experiment was conducted with cells from another, single donor.…”

Section: Surface Propertiesmentioning

confidence: 99%

Ternary composite scaffolds with tailorable degradation rate and highly improved colonization by human bone marrow stromal cells

Idaszek

Bruinink

Święszkowski

2014

J. Biomed. Mater. Res.

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Poly(ε-caprolactone), PCL, is of great interest for fabrication of biodegradable scaffolds due to its high compatibility with various manufacturing techniques, especially Fused Deposition Modeling (FDM). However, slow degradation and low strength make application of PCL limited only to longer-term bioresorbable and non-load bearing implants. To overcome latter drawbacks, ternary PCL-based composite fibrous scaffolds consisting of 70-95 wt % PCL, 5 wt % Tricalcium Phosphate (TCP) and 0-25 wt % poly(lactide-co-glycolide) (PLGA) were fabricated using FDM. In the present study, the effect of composition of the scaffolds on their mechanical properties, degradation kinetics, and surface properties (wettability, surface energy, and roughness) was investigated and correlated with response of human bone marrow mesenchymal stromal cells (HBMC). The presence of PLGA increased degradation kinetics, surface roughness and significantly improved scaffold colonization. Of the evaluated surface properties only the wettability was correlated with the surface area colonized by HBMC. This study demonstrates that introduction of PLGA into PCL-TCP binary composite could largely abolish the disadvantages of the PCL matrix and improve biocompatibility by increasing wettability and polar interactions rather than surface roughness. Additionally, we showed great potential of multicellular spheroids as a sensitive in vitro tool for detection of differences in chemistry of 3D scaffolds.

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“…[7] Furthermore, the haemolytic activity is decreased and part of the viruses and micro-organisms are destroyed. [8] Since components are not added or removed, but only altered (protein aggregation/denaturation [9] ), this procedure does not solve the problem of initial variations in the FBS composition. On the contrary, the rate of denaturation can represent an additional unknown variable.…”

Section: Introductionmentioning

confidence: 99%

Direct and Protein‐Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond

Klauser

Hermann

Steinmüller‐Nethl³

et al. 2010

Chemical Vapor Deposition

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The aim of this study is to investigate the influence of wettability on direct and protein-mediated cell-surface interactions. Nanocrystalline diamond (NCD) is chosen as a cell growth substrate, because it offers the possibility to adjust surface properties like roughness or chemical termination, thus realizing hydrophilic or hydrophobic surfaces. Cell adhesion is studied on NCD films with a defined roughness but different surface chemistries (hydrogen, fluorine, and oxygen terminations) using two different cell lines (LLC-PK1 and PANC-1). They are cultured with and without the addition of fetal bovine serum (FBS) to the growth medium in order to discriminate between direct and mediated cell adhesion. For both cell lines, the cells are not able to adhere to hydrophobic surfaces without the addition of FBS to the growth medium, whereas they can attach in the presence of FBS. Hydrophilic surfaces enable cell attachment with and without the addition of FBS to the medium.

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Effects of serum and serum heat-inactivation on human bone derived osteoblast progenitor cells

Cited by 34 publications

References 18 publications

Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture

Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture

Ternary composite scaffolds with tailorable degradation rate and highly improved colonization by human bone marrow stromal cells

Direct and Protein‐Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond

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